Báo cáo khoa học: ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by ppt

... ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by copurification of adenylate kinase of Escherichia coli Baoyu Chen 1, *, ... adenylate kinase (AK). Apparent catalysis of ADP hydrolysis by NtrC1 C was in fact conversion of two ADP molecules to ATP and AMP by AK followed by hydrolysis of ATP to ADP and P i by NtrC1 C . Pro...

Ngày tải lên: 16/03/2014, 04:20

... could be converted into a PK-resistant isoform in the presence of NADPH. Aliquots of ScBH of SHa-263K were serially diluted into solutions of rSHaPrP or rHu- PrP, with final ScBH dilutions of 10 )3 ,10 )4 ,10 )5 and ... microplate horn for PCR tubes. Ninety microliters of NBH or 0.2 mgÆmL )1 rPrP were mixed with 10 lL of vari- ous concentrations of ScBH in a volume of 100 l...

Ngày tải lên: 07/03/2014, 03:20

... the case of mGFP–calpain 7 C290S , suggesting that the degradation was caused by proteo- lytic activity of mGFP–calpain 7 itself. We refer to this issue later. Effects of mutations of IST1 MIMs ... ATPase activity of VPS4 ⁄ Vps4 [32,33,37]. Because of the structural and functional resemblance to CHMP proteins, IST1 is now regarded as a new ESCRT-III family member. The findi...

Ngày tải lên: 18/02/2014, 04:20

... easily explained by the absence of XAN-H protein. In the Xantha-h clo 125 , -h clo 157 and -h clo 161 mutants, however, the lack of XAN-G has to be explained by an inhibited activity of the deficient ... it has been shown for several AAA + proteins that a significant change of conformation occurs during the ATP hydrolysis cycle and it has been suggested that this may be a ge...

Ngày tải lên: 19/02/2014, 12:20

... used by serine pro- teases such as a-chymotrypsin. Enzymes of this type are generally considered to be inactive at pH values below 5, because the histidine member of the catalytic triad becomes protonated. ... low- pH activity of wild-type BuChE in the presence of excess BTC is not supported. Moreover, it should be noted that the pK a of mutant E1997Q for k cat is shifted b...

Ngày tải lên: 20/02/2014, 23:20

... signals [3,4]. The evolutionary expansion of the plant sHsp family may be the result of selection pressure for tolerance to the many types of stresses encountered by plants when they made the transition ... of its chaper- one activity has been performed. Wheat TaHsp17.8C-II is  33% identical overall to TaHsp16.9C-I. Only two previ- ous studies have examined the chaperone act...

Ngày tải lên: 07/03/2014, 15:20

... antimicrobial activity and represent a d ef- ense mechanism previously unreported in an invertebrate. Histones may be a component of innate immunity more widely conserved, and of earlier origin, ... cascade leading to melanization has been one of the most studied immune reactions in crustaceans [6]. Melanin and its intermediates have been shown to be toxic to microbes [7]. Antim...

Ngày tải lên: 07/03/2014, 16:20

... of 2 lL of 10% SDS. The samples were boiled for 10 min and separated by 15% SDS/PAGE. Analysis was by autoradiography. Enzymatic activity of Ndk Enzymatic activity of purified Ndk or its activity ... detection of Ndk in the culture supernatant of M. tuberculosis H37Rv is caused by the secretion rather than by the autolysis of the cells, culture supernatant was als...

Ngày tải lên: 08/03/2014, 08:20

... pres- ence of saturating concentrations of dUMP, to ensure examination of the dUMP-activated form of the enzyme [12,13]. The results revealed that, in the absence of CH 2 H 4 folate, FDTS oxidase activity exhibits ... 0.02 lm. Assessment of the rate of product NADP + release by examination of the FADH 2 –NADP + charge-transfer complex The progress of the reductive half-r...

Ngày tải lên: 23/03/2014, 04:21

... presence of 4 l M pre-EF-Tu, 46% of CS activity remained after 30 min (Fig. 6 A). During the recovery period, the activity of C S gradually increased, reaching a maximum of 68% of its original activity ... kDa HSP because the identity of this protein was not known until the report of Bhadula et a l.[5]). One could argue that the chaperone activity observed in our study...

Ngày tải lên: 23/03/2014, 13:20