Báo cáo khoa học: Functional significance of five noncanonical Ca2+-binding sites of human transglutaminase 2 characterized by site-directed mutagenesis potx
Báo cáo khoa học: Functional significance of five noncanonical Ca2+-binding sites of human transglutaminase 2 characterized by site-directed mutagenesis potx
... authors. Ca 2+ -binding sites of TG2 R. Kira ´ ly et al. 7096 FEBS Journal 27 6 (20 09) 7083–7096 ª 20 09 The Authors Journal compilation ª 20 09 FEBS Functional significance of five noncanonical Ca 2+ -binding sites ... 20 0 21 0 22 0 23 0 24 0 25 0 –10 –5 0 5 10 15 [θ] x 10 –3 deg cm 2 decimol –1 Wavelength (nm) S5 S4 W S2A S1, S2B S3A,S3B Fig. 3. CD spectra...
Ngày tải lên: 16/03/2014, 03:20
Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx
... dependencies of inhibitor-free epimerase preincubated with 0 .2 m M UDP + 2 mMD(+)-Gal. Regulation of catalytic sites of yeast epimerase A. Brahma et al. 6734 FEBS Journal 27 6 (20 09) 6 725 –6740 ª 20 09 ... folin-phenol reagent. J Biol Chem 193, 26 5 27 6. Regulation of catalytic sites of yeast epimerase A. Brahma et al. 6740 FEBS Journal 27 6 (20 09) 6 725 –6740 ª 20...
Ngày tải lên: 16/03/2014, 00:20
Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... sulfone [R–S(O 2 )–R¢] [20 ], sulfonate ester [R–S(O 2 )–O–R¢] [21 ,22 ], sulfonamide [R–S(O 2 )–NH– R¢] [23 ], sulfamate [R–O–S(O 2 )–NH–R¢] [24 ], sulfamide [R–NH–S(O 2 )–NH–R¢] [25 ,26 ], and N -acylsulfamate [R–O–S(O 2 )–NH–C(O)–R¢] ... (2. 8) 3.1 (2. 9) Wilson B-factor (A ˚ 2 ) 17.8 18.1 R cryst ⁄ R free 0 .21 2 ⁄ 0 .24 6 0 .21 4 ⁄ 0 .24 4 Average B-factor (A ˚ 2 ) Overal...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... related Fe 2+ sites of CDO [27 ], human pirin [25 ,26 ], and gentisate 1 ,2- dioxygenase [22 ,23 ], cannot be definitely ruled out. The modeled structure of the nonheme metal site of Bxe_A2876 (Fig. ... number of the Fe 2+ center [29 –31]. The value of ( 12 ± 1) · 10 )2 eV there- fore indicates that the Fe 2+ bound to Bxe_A2876 is coordinated by a total of five li...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... K m (s )1 ÆM )1 ) Wild-type a dThd 1 .2 29.5 14 .2 1 .2 · 10 7 (100%) dCyd 2. 3 34 .2 16 .2 7.0 · 10 6 (100%) AraC b 24 .3 5.6 2. 9 1 .2 · 10 5 AraT 62 ± 7.4 10.4 ± 0.7 0.3 7 · 10 4 BVDU 2. 2 ± 0.1 13 .2 ± 1.7 5.9 2. 6 · 10 6 E52D dThd ... 0.001 62 ± 0.000 02 7 .2 · 10 )4 1.9 · 10 2 (< 1%) dCyd 3.7 ± 0.4 0.00160 ± 0.00018 7.1 · 10 )4 1.9 · 10 2 (< 1%) E52H dThd* 3.7...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... purification procedure of fusion proteins with g lutathione S-transferase. Anal. Biochem. 1 92, 26 2 26 7. 22 . Yun, Z., Maecker, H.L., Johnson, R.S. & Giaccia, A.J. (20 02) Inhibition of PPAR gamma 2 gene expression ... March 20 04, revised 14 September 20 04, accepted 24 September 20 04) Eur. J. Biochem. 27 1, 4409–4419 (20 04) Ó FEBS 20 04 doi:10.1111/j.14 32- 1033 .20 04...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis docx
... Chem 26 8, 121 50– 121 55. 40 Vali Z & Patthy L (19 82) Location of the intermediate and high affinity omega-aminocarboxylic acid-binding sites in human plasminogen. J Biol Chem 25 7, 21 04 21 10. 41 ... to the study of the activation pathways of plasminogen in vivo. J Biol Chem 26 0, 121 06– 121 11. 57 Wiman B & Wallen P (1973) Activation of human plas- minogen by...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... signal. (Received 21 February 20 03, revised 28 April 20 03, accepted 12 May 20 03) Eur. J. Biochem. 27 0, 28 37 28 47 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03661.x recombination or repair [16]. Ydc2-deficient ... Fujifilm FLA -20 00 phosphoimager. Immunofluorescence microscopy S. pombe strain JAL01 was transformed by the lithium chloride method [29 ] with pREP41–EGFP...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx
... June 20 03, revised 26 August 20 03, accepted 8 September 20 03) Eur. J. Biochem. 27 0, 429 4–4305 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03 822 .x complex used in the classical model of the ... phaseÕ of growth, is 78% of the point of growth inhibition of UY211 (D 420 ¼ 0.60). The point of growth inhibition of GRd.tsf when expressed as a percentage of t...
Ngày tải lên: 21/02/2014, 00:20
Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx
... domes- tica, AAD2 626 1; Matthiola incana, O04395. (Received 17 April 20 02, revised 4 July 20 02, accepted 11 July 20 02) Eur. J. Biochem. 26 9, 4134–41 42 (20 02) Ó FEBS 20 02 doi:10.1046/j.14 32- 1033 .20 02. 03108.x Several ... (His 221 , His277) and one acidic amino acid (Asp 223 ) residue for Fe II -coordination, an arginine (Arg287) proposed to bind 2- oxoglutarate, and four a...
Ngày tải lên: 21/02/2014, 03:20