Báo cáo khoa học: Functional signiﬁcance of ﬁve noncanonical Ca2+-binding sites of human transglutaminase 2 characterized by site-directed mutagenesis potx

... authors.Ca 2+ -binding sites of TG2 R. Kira´ly et al.7096 FEBS Journal 27 6 (20 09) 7083–7096 ª 20 09 The Authors Journal compilation ª 20 09 FEBS Functional signiﬁcance of ﬁve noncanonical Ca 2+ -binding sites ... 20 0 21 0 22 0 23 0 24 0 25 0–10–5051015[θ] x 10–3 deg cm 2 decimol–1Wavelength (nm)S5S4WS2AS1, S2BS3A,S3BFig. 3. CD spectra of recombinant TG2 proteins.R. Kira´ly et al. Ca 2+ -binding ... Multiple sequence alignment of Ca 2+ -binding sites of transglutaminases. Sequence alignments of Ca 2+ -binding sites of TG2 com-pared with the other members of transglutaminase family usingCLUSTALW....

Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

... dependencies of inhibitor-freeepimerase preincubated with 0 .2 mM UDP + 2 mMD(+)-Gal.Regulation of catalytic sites of yeast epimerase A. Brahma et al.6734 FEBS Journal 27 6 (20 09) 6 725 –6740 ª 20 09 ... folin-phenolreagent. J Biol Chem 193, 26 5 27 6.Regulation of catalytic sites of yeast epimerase A. Brahma et al.6740 FEBS Journal 27 6 (20 09) 6 725 –6740 ª 20 09 Council of Scientiﬁc and Industrial Research, ... originalplot.Regulation of catalytic sites of yeast epimerase A. Brahma et al.6 728 FEBS Journal 27 6 (20 09) 6 725 –6740 ª 20 09 Council of Scientiﬁc and Industrial Research, New Delhi. Journal compilation ª 20 09 FEBScompleted...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... sulfone [R–S(O 2 )–R¢] [20 ], sulfonate ester[R–S(O 2 )–O–R¢] [21 ,22 ], sulfonamide [R–S(O 2 )–NH–R¢] [23 ], sulfamate [R–O–S(O 2 )–NH–R¢] [24 ], sulfamide[R–NH–S(O 2 )–NH–R¢] [25 ,26 ], and N -acylsulfamate[R–O–S(O 2 )–NH–C(O)–R¢] ... (2. 8) 3.1 (2. 9)Wilson B-factor (A˚ 2 ) 17.8 18.1Rcryst⁄ Rfree0 .21 2 ⁄ 0 .24 6 0 .21 4 ⁄ 0 .24 4Average B-factor (A˚ 2 )Overall 18.1 18.3Protein (chain A, B) 16 .2, 16.5 16.4, 16.3Ligand 21 .8, ... 0.0 62 (0.1 92) I ⁄ rI (outer shell) 17.5 (6.0) 17 .2 (5.7)Completeness (outer shell) (%) 98.5 (94.5) 98.0 ( 92. 7)Total no. of reﬂections 174 818 186 775Unique no. of reﬂections 26 158 26 20 0Redundancy...

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... related Fe 2+ sites of CDO [27 ], human pirin [25 ,26 ], and gentisate 1 ,2- dioxygenase [22 ,23 ], cannot be deﬁnitely ruled out. Themodeled structure of the nonheme metal site of Bxe_A2876 (Fig. ... number of the Fe 2+ center [29 –31]. The value of ( 12 ± 1) · 10 )2 eV there-fore indicates that the Fe 2+ bound to Bxe_A2876 iscoordinated by a total of ﬁve ligand atoms.An initial estimation of ... (e 28 0) were used [28 ]: 2, 4-pentanedi-one, 22 40 m)1Æcm)1; 3,5-heptanedione, 120 0 m)1Æcm)1; 2, 4-octanedione, 120 0 m)1Æcm)1; and 2- acetylcyclohexanone, 24 00 m)1Æcm)1. Values of...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... Km(s)1ÆM)1)Wild-typeadThd 1 .2 29.5 14 .2 1 .2 · 107(100%)dCyd 2. 3 34 .2 16 .2 7.0 · 106(100%)AraCb 24 .3 5.6 2. 9 1 .2 · 105AraT 62 ± 7.4 10.4 ± 0.7 0.3 7 · 104BVDU 2. 2 ± 0.1 13 .2 ± 1.7 5.9 2. 6 · 106E52D dThd ... 0.001 62 ± 0.000 02 7 .2 · 10)41.9 · 10 2 (< 1%)dCyd 3.7 ± 0.4 0.00160 ± 0.00018 7.1 · 10)41.9 · 10 2 (< 1%)E52H dThd* 3.7 0. 026 12 1 .2 · 10 )2 3 .2 · 103(< 1%)dCyd 5.8 ± 2. 0 0.0 025 9 ... Anal Biochem 72, 24 8 25 4. 22 Knecht W, Bergjohann U, Gonski S, Kirschbaum B &Lofﬂer M (1996) Functional expression of a fragment of human dihydroorotate dehydrogenase by means of thebaculovirus...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... puriﬁcationprocedure of fusion proteins with g lutathione S-transferase. Anal.Biochem. 1 92, 26 2 26 7. 22 . Yun, Z., Maecker, H.L., Johnson, R.S. & Giaccia, A.J. (20 02) Inhibition of PPAR gamma 2 gene expression ... March 20 04, revised 14 September 20 04,accepted 24 September 20 04)Eur. J. Biochem. 27 1, 4409–4419 (20 04) Ó FEBS 20 04 doi:10.1111/j.14 32- 1033 .20 04.04379.xmice [11, 12, 15], whereas overexpression of ... (BMAL1:1 12 626 ) did not affect thebinding of BMAL1 to DEC1; deletion of 23 5 amino acids(BMAL1 :23 6– 626 ) slightly diminished the binding,although a strong binding ability of BMAL1 :23 6– 626 withDEC1...

Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... Chem 26 8, 121 50– 121 55.40 Vali Z & Patthy L (19 82) Location of the intermediateand high afﬁnity omega-aminocarboxylic acid-binding sites in human plasminogen. J Biol Chem 25 7, 21 04 21 10.41 ... tothe study of the activation pathways of plasminogen invivo. J Biol Chem 26 0, 121 06– 121 11.57 Wiman B & Wallen P (1973) Activation of human plas-minogen by an insoluble derivative of urokinase. ... the C-ter-minal region [26 ,27 ] and between K3 and K4 [28 ],organized in a spiral-type domain conformation [26 ,29 – 32] . Rupture of these interactions by proteolyticremoval of the N-terminal peptide...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... signal.(Received 21 February 20 03, revised 28 April 20 03,accepted 12 May 20 03)Eur. J. Biochem. 27 0, 28 37 28 47 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03661.xrecombination or repair [16]. Ydc2-deﬁcient ... FujiﬁlmFLA -20 00 phosphoimager.Immunoﬂuorescence microscopyS. pombe strain JAL01 was transformed by the lithiumchloride method [29 ] with pREP41–EGFP [28 ], pMW217[16], pBS201, pBS2 02, pBS203 or pBS204. ... previously [ 12, 13 ,23 ]. Complex Icontains one dimer of Ydc2 while complex II most likelyresults from the binding of two dimers of Ydc2 and its functional signiﬁcance is doubtful [14]. The Ydc2-ND35mutant...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... June 20 03, revised 26 August 20 03,accepted 8 September 20 03)Eur. J. Biochem. 27 0, 429 4–4305 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03 822 .xcomplex used in the classical model of the ... phaseÕ of growth, is 78% of the point of growthinhibition of UY211 (D 420 ¼ 0.60). The point of growthinhibition of GRd.tsf when expressed as a percentage of that of UY211, was independent of the ... EF-Tsmt(residues 23 2 23 7) [31] and human EF-Tsmt(residues 24 3 24 8) [28 ], was inserted as alinker (Figs 1 and 2) .Chromosomal DNA from E. coli strain UY211 (ara,D(lac-pro), nalA, thi) [ 32] was isolated...

Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of ﬂavonol synthase from Citrus unshiu pptx

... domes-tica, AAD2 626 1; Matthiola incana, O04395.(Received 17 April 20 02, revised 4 July 20 02, accepted 11 July 20 02) Eur. J. Biochem. 26 9, 4134–41 42 (20 02) Ó FEBS 20 02 doi:10.1046/j.14 32- 1033 .20 02. 03108.xSeveral ... (His 221 , His277) and one acidic amino acid(Asp 223 ) residue for FeII-coordination, an arginine (Arg287)proposed to bind 2- oxoglutarate, and four amino acids(Gly68, His75, Gly261, Pro207) ... of most of the conserved amino acids hasbeen further substantiated by site-directed mutagenesis of FHT [7,9] and by documentation of ligand binding incrystalline DAOCS and IPNS complexes [23 25 ]....

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