Báo cáo khoa học: Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals pdf

... FEBS Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals Ga ´ bor Mere ´ nyi 1 , Emese Ko ´ nya 1 and Bea ´ ta G. Ve ´ rtessy 1,2 1 Institute ... Importin a possesses two major domains for its adaptor function, the importin b binding (IBB) domain in its N-terminus and the C-terminal NLS- binding domain....

Ngày tải lên: 15/03/2014, 11:20

... to both Swi6 and Sin3 [20,21,32,34]. In fact, Stb1 was originally identified as an interacting protein of Sin3 in two-hybrid assays (Stb1 for Sin three binding) [34]. STB1 transcription was shown ... is inactivated [33]. Phosphory- lation of Stb1 inhibits interaction between Swi6 and Stb1 [20]. Our finding of cell-cycle-specific promoter binding by Sin3 is in agreement with the propo...

Ngày tải lên: 23/03/2014, 05:22

... was 0.5–2 mLÆmin )1 . Extraction of archaeol from H. salinalium H. salinarum was cultured in 1 L of culture medium, con- taining 5 g of casamino acids, 5 g of yeast extract, 3 g of Archaetidic ... quantitation of microgram quantities of protein util- izing the principle of protein-dye binding. Anal Biochem 72, 248–254. 31 Fujii H, Koyama T & Ogura K (1982) Efficient enzy-...

Ngày tải lên: 30/03/2014, 09:20

... uncoupling protein 1. Brown adipose tissue (BAT) and white adipose tissue (WAT) both play an important role in the control of energy balance in mammals. Indeed, BAT is involved in the control of ... Ten days of cold-exposure increased the number of multilocular cells and induced the expression of UCP1 in some of them in both WT and b 3 KO mice. Mice maintained singly h...

Ngày tải lên: 23/03/2014, 20:22

... Schiffmann SN (2002) ‘New’ functions for ‘old’ proteins: The role of the calcium- binding proteins calbindin D-28k, calretinin and parval- bumin, in cerebellar physiology. Studies with knockout mice. ... and Ca 2+ fluxes [8]. An increased biogenesis of mitochondria was detected in skeletal muscle of null-mutant mice for proteins involved in ATP metabolism, such as creatine...

Ngày tải lên: 19/02/2014, 07:20

... 5-fluoro-2¢-deoxyuridine; 5-I-dUrd, 5-iodo-2¢-deoxyuridine; ACV, acyclovir; araC, 1-b- D-arabinofuranosylcytosine; araG, 9-b-D-arabinofuranosylguanine; araT, 1-b-D-arabin- ofuranosylthymine; BVaraU, ... caused by mutations in the nuclear gene for thymidine phosphorylase, which results in increased levels of thymidine. This enzyme catalyzes the phosphorolysis of thymidine to thymine a...

Ngày tải lên: 20/02/2014, 01:20

... starch-binding ability of AtAMY3 in vitro. It cannot be precluded that some binding may involve secondary binding sites in the catalytic domain, but the demonstrated starch- binding ability of different ... as a starch-binding domain (SBD) [3]. CBMs are noncatalytic structural domains that mediate the binding of proteins to polysaccharides, thus bringing the appended catalytic m...

Ngày tải lên: 06/03/2014, 00:21

... SHBs in general are found to be involved in stabilizing the three dimensional structures of proteins at specific structural locations as discussed above. A single protein such as copper amine oxidase Asp ... Hydrogen bonds in the alpha helices of globular proteins. Biophy- sics 46, 969–977. 35 Wilmot CM & Thornton JM (1988) Analysis and pre- diction of the different typ...

Ngày tải lên: 07/03/2014, 17:20

... presence of three protein bands. The position of the main band is in agreement with the predicted molecular mass of the hexahistidine fusion protein (27 kDa). The N-terminal amino acid sequence of ... tuberculosis H37Rv Fig. 6. Neighbor joining tree for 929 LmbE proteins. Bacillus cereus proteins and pro- teins of known function are indicated. A. Deli et al. LmbE proteins fro...

Ngày tải lên: 15/03/2014, 11:20

... identical to the corresponding DNA-binding domain of orthologous Six3 proteins in other verte- brates, displayed differential binding affinities for vari- ous sites containing a TAAT core motif, and ... specifically favouring binding to Six3 proteins were not identified. Our examination of the interaction of zebrafish Six3a with 43 TAAT-containing sequences, distributed within the pro...

Ngày tải lên: 29/03/2014, 08:20