Báo cáo khoa học: The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs pdf

... structure of the ROQ domain, impairingconcomitant binding of Roquin and miRNA to the target mRNA. Elucidating how Roquin regulates the metabolism of target mRNAs in stress granules maytherefore ... retroviral vector [1], to expresseither Roquin WT, Roquin M199Ror the Roquin constructlacking the ROQ domain – Roquin DROQ– ectopicallyin roquin san ⁄ sanCD4+T cells. Although Roquin WTreduces ... negativeover Roquin WT, at least in EL4 cells. Taken together,these results show that the ROQ domain is essentialfor Roquin s repression of Icos mRNA. Roquin binds Icos mRNA To determine whether Roquin...

Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt

... Choice of the aforementioned sequence is because of the provenefﬁcacy of the designed PNA to down-regulate the gene expression of the mutated allelle, while sustaining the transcription of the ... prevails at the AC(6–7) step (Fig. 2A) of PDwt, and the AT(6–7) step of PDmut(Fig. 2E). Likewise, the extent of intra strand base stacking at the GA(23–24) and AT(24–25) steps of the PNA strand in ... 2B), and the TC(7–8) step of PDmut(Fig. 2F) of the DNAstrand show signiﬁcant differences. This is due to con-siderable movement of cytosine (C7) of the A24 C7mismatch of PDwt, leading to large...

Báo cáo khoa học: C Isotopologue editing of FMN bound to phototropin domains potx

... modula-tion of the FMN cofactors complexed to LOVdomains of different origins. For the unequivocalassignment of the 13C NMR signals of all carbonatoms in the FMN chromophore, the apoproteins ... [4]. In the present study, we report the transfor-mation of these isotopologue libraries into randomlibraries of 13C isotopologues of FMN and their utili-zation for NMR studies of the plant ... conducive to the addition of a cysteinyl thiolate group to carbon 4a of the FMN chromophore, and spontaneous cleavage of that photoadduct completes the photocycle of the receptor. The present...

Báo cáo khoa học: Factors affecting habituation of PC12 cells to ATP potx

... loop to the cell chamber. At a ﬂow rate of 1mLÆmin)1, the cells were stimulated for  6s. Norepi-nephrine standards were used to determine the response of the d etector and the dispersion of ... ultiplying the effect of each condition to a stimulus (B/A) by the ratio of the cellular response of that stimulus to 300 lMATP (F/G). The term Ôscaled responseÕ will refer to the response of PC12cells ... deﬁned as the ratios of the amplitude of each response (B,C,D,E) in the run to the amplitude of the initial r esponse of that run (B). Habituation w ill be recordedin text as a percentage of the fourth...

Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx

... protons of GlcNAc, and the H3-equitorial and H3-axial protons of N-glycolylneuraminic acid[23–26]. The 1H-NMR spectrum of cRBCs within the region of interest is shown in Fig. 3 and the list of important ... and MSnon the topeight most intense ions. Ions from m ⁄ z 480–2000 wereselected.MALDI-TOF/TOFIn addition to LC-MS ⁄ MS analysis, we also completedTOF ⁄ TOF analysis of selected peaks to ... treatment of the collected pools to determine the distribution of sialicacid. Next, we completed online MS ⁄ MS analysis of the major species; some of these species were furtheranalyzed by TOF ⁄ TOF...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... independently of Pbx, and does notpossess the autoinhibitory function of the Hth domain. In summary, our data suggest that one function of the conserved Hth domain is to inhibit the activity of the transcriptional ... region 2 (hr2) of the Hth domain, derepressed it to a similar degree as the fullHth deletion (Fig. 4A). To test whether the inhibitoryactivity of the Hth domain was speciﬁc to the Meis2AD, ... by deletion of the Hth domain, suggestingthat the inhibitory effect of this domain is notspeciﬁc to the Meis2d AD. Comparison of the relativeexpression levels of these GBD fusion proteins (seeFig....

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... (I6–V26) and the extremely acidic cytoplasmic domain (I39–L81). The transmembrane domain consists of a well-characterized and deﬁned a-helix (referred to as helix 1) [17–20]. The structure of the cytoplasmic ... elements and, possibly, a ter-tiary fold of the cytoplasmic domain of VpU remainshighly debated. To date, the most detailed descriptions of the soluble VpU region are based on proton–protondistances ... tertiary fold in the cytoplasmicregion of VpU, as indicated by a small number of observed long-range NOEs [24].Another important aspect is the topology of the cytoplasmic domain of VpU on a membrane....

Tài liệu Báo cáo khoa học: Substrate speciﬁcity of the pseudouridine synthase RluD in Escherichia coli doc

... obtained with the transition at posi-tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the othertwo ... of the wild-type 23SrRNA from the 50S and the 70S particles were similar:stop sites were found at positions 1911 and 1917 on the CMCT treated RNA and not on the control RNA(wild-type 70S and ... Y1911 of the variants A1913G and A1918G is very weak due to strong stop sites at 1917 and 1915 (Fig. 4). Longer exposure revealed the pres-ence of a Y speciﬁc band at position 1911 (not shown).The...

Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf

... stroke and Huntington’sdisease, and 4-halohydroxyanthranilates have beenfound to be speciﬁc and potent HAD inhibitors. To check for covalent modiﬁcations of the enzyme, the effect of the inhibitor ... a molecular mass of 16 309.7 Da, butthis matched none of the DNA-predicted proteins. MS ⁄ MS of these ions gave the C-terminal sequence of Fig. 5. These and all other peaks of that spectrum ... cleavages in the center of the protein to formadditional loose ends to be denatured out of the ball of spaghetti. The ESI spectrum of the 200 kDa human comple-ment C4 glycoprotein (of 1714 residues...

Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx

... major role of Arg316 in arresting the ligandWhen the amino acid sequences of the LBD of all the NRs were aligned to that of ERRc, it became notice-able that 26 receptors among the total 48 NRs ... the cells were trea-ted with a vehicle solution to measure the basal con-stitutive activity of each receptor, by using exactly the same of amount of expression plasmid of the receptor.Furthermore, ... chains of Glu and Arg, the mutant recep-tors are devoid of the functional groups at the particular positions of 275 and 316. Thus, it becomesdifﬁcult for them to keep BPA in the ligand-bindingpocket.Interestingly,...

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