Báo cáo khoa học: The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs pdf

... structure of the ROQ domain, impairing concomitant binding of Roquin and miRNA to the target mRNA. Elucidating how Roquin regulates the metabolism of target mRNAs in stress granules may therefore ... retroviral vector [1], to express either Roquin WT , Roquin M199R or the Roquin construct lacking the ROQ domain – Roquin DROQ – ectopically in...

Ngày tải lên: 15/03/2014, 11:20

... Choice of the aforementioned sequence is because of the proven efficacy of the designed PNA to down-regulate the gene expression of the mutated allelle, while sustaining the transcription of the ... prevails at the AC(6–7) step (Fig. 2A) of PD wt , and the AT(6–7) step of PD mut (Fig. 2E). Likewise, the extent of intra strand base stacking at the GA(23...

Ngày tải lên: 07/03/2014, 21:20

... modula- tion of the FMN cofactors complexed to LOV domains of different origins. For the unequivocal assignment of the 13 C NMR signals of all carbon atoms in the FMN chromophore, the apoproteins ... [4]. In the present study, we report the transfor- mation of these isotopologue libraries into random libraries of 13 C isotopologues of FMN and their utili- zatio...

Ngày tải lên: 07/03/2014, 05:20

... loop to the cell chamber. At a flow rate of 1mLÆmin )1 , the cells were stimulated for  6s. Norepi- nephrine standards were used to determine the response of the d etector and the dispersion of ... ultiplying the effect of each condition to a stimulus (B/A) by the ratio of the cellular response of that stimulus to 300 l M ATP (F/G). The term Ôscaled res...

Ngày tải lên: 07/03/2014, 16:20

... protons of GlcNAc, and the H3-equitorial and H3-axial protons of N-glycolylneuraminic acid [23–26]. The 1 H-NMR spectrum of cRBCs within the region of interest is shown in Fig. 3 and the list of important ... and MS n on the top eight most intense ions. Ions from m ⁄ z 480–2000 were selected. MALDI-TOF/TOF In addition to LC-MS ⁄ MS analysis, we also completed TOF...

Ngày tải lên: 14/02/2014, 19:20

... independently of Pbx, and does not possess the autoinhibitory function of the Hth domain. In summary, our data suggest that one function of the conserved Hth domain is to inhibit the activity of the transcriptional ... region 2 (hr2) of the Hth domain, derepressed it to a similar degree as the full Hth deletion (Fig. 4A). To test whether the inhibitory a...

Ngày tải lên: 16/02/2014, 15:20

... (I6–V26) and the extremely acidic cytoplasmic domain (I39–L81). The transmembrane domain consists of a well-characterized and defined a-helix (referred to as helix 1) [17–20]. The structure of the cytoplasmic ... elements and, possibly, a ter- tiary fold of the cytoplasmic domain of VpU remains highly debated. To date, the most detailed descriptions of the...

Ngày tải lên: 18/02/2014, 06:20

... obtained with the transition at posi- tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the other two ... of the wild-type 23S rRNA from the 50S and the 70S particles were similar: stop sites were found at positions 1911 and 1917 on the CMCT treated RNA and not on...

Ngày tải lên: 18/02/2014, 16:20

... stroke and Huntington’s disease, and 4-halohydroxyanthranilates have been found to be specific and potent HAD inhibitors. To check for covalent modifications of the enzyme, the effect of the inhibitor ... a molecular mass of 16 309.7 Da, but this matched none of the DNA-predicted proteins. MS ⁄ MS of these ions gave the C-terminal sequence of Fig. 5. These and...

Ngày tải lên: 18/02/2014, 16:20

... major role of Arg316 in arresting the ligand When the amino acid sequences of the LBD of all the NRs were aligned to that of ERRc, it became notice- able that 26 receptors among the total 48 NRs ... the cells were trea- ted with a vehicle solution to measure the basal con- stitutive activity of each receptor, by using exactly the same of amount of expression...

Ngày tải lên: 18/02/2014, 16:20