Báo cáo khoa học: Cross-species divergence of the major recognition pathways of ubiquitylated substrates for ubiquitin⁄26S proteasome-mediated proteolysis potx

... a cross-species mechanistic and functional divergence of the major recognition pathways for the ubiquitylated substrates of UPP. Results Divergence of the ubiquitin binding properties of the major ... loss of function support a major role for Arabdopsis RPN10 in the direct and indirect recognition of ubiquitylated substrates The multiplici...

Ngày tải lên: 15/03/2014, 09:20

... PARG isoforms tar- geted either to the nucleus or to the cytoplasm [47]. Overexpression studies revealed that the largest iso- form of PARG is targeted to the nucleus while the two smaller isoforms ... within the known members of the PARP family. Adjacent to the ANK domain is another protein interaction motif, the sterile alpha- module. The C-terminus of tankyrase di...

Ngày tải lên: 20/02/2014, 01:20

... sub population of enzyme binds directly the steroid in the reactive conformation, with the remainder of the enzyme binding the unreactive conformation. Rescue of the unreactive conformation involves ... re-oxidation of the flavin and the formation of the hydride-Meisenheimer complex of TNT. (C) As for panel B except the spectral changes are recorded over an ex...

Ngày tải lên: 20/02/2014, 02:21

... Ssa1p binds a hexameric form of Ure2p in the presence of ATP, whereas the form that is bound in the presence of ADP is different, and probably dimeric [6]. We there- fore performed Ure2p and Ssa1p ... built from the X-ray structure of the C-terminal domain of Ure2p and integrates the finding that the N-terminal domain of Ure2p is flexible. An enlargement of the reg...

Ngày tải lên: 15/03/2014, 00:20

... [10,11]. Biochemical analyses of LtrA mutants (the IEP of the Ll.ltrB intron of Lactococcus lactis) have suggested that the N-terminus of the RT domain is required for protein interactions with the high-affinity ... region. Effect of mutations in the C-terminal region of the RmInt1 IEP on RNA splicing in vivo We constructed a series of mutants to identify the fun...

Ngày tải lên: 15/03/2014, 09:20

... duplicate copies within the C1 com- plex, forming a tetramer. Both the autoactivation of C1r and the activation of C1s require large-scale move- ments of the different domains of the proteases. Inter- modular ... FEBS under the conditions applied and therefore the constructs could be reliably used to assess the interde- pendence of the modules. This was corroborate...

Ngày tải lên: 15/03/2014, 23:20

... to the affinity of nitrite for the protein site where it is transformed into the nitrating species, in the presence of the phenol. Interestingly, these constants resemble the reciprocal of the nitrite ... Taglietti (University of Pavia) for assistance in the stopped-flow experiments, and Antonella Profumo (University of Pavia) for the determination of the r...

Ngày tải lên: 16/03/2014, 16:20

... it is therefore likely that the outcome of the inhibition of aggrecanase activity in tendon is the retention of large proteoglycans within the extracellular matrix of the tissue [22–27]. The mechanism ... inclusion of these sulfated agents there was significant inhibition (40%) of rate of loss of 35 S-labeled large proteogly- cans from the matrix by the end of...

Ngày tải lên: 23/03/2014, 10:21

... rates for the code can be higher than the numbers of forms in the full-form lexicon is explained by the fact that the gener- ated forms are longer than the base forms. For instance, the full-form ... http://www.eecs. qmul.ac.uk/ ˜ mpurver/software.html one third of the nouns of the lexicon were not in- cluded in the experiment because one of the form was miss...

Ngày tải lên: 24/03/2014, 03:20

... meaningful informa- tion on the thermodynamic stability of the Ca 2+ - and Zn 2+ -loaded mutants can be retrieved from the anal- ysis of metallation effects within the background of the same mutation ... aggregation. The formation of insoluble b-sheet-containing aggregates is most certainly an important contributor to the irreversibility of the ther- mal denaturation...

Ngày tải lên: 30/03/2014, 02:20