... includingcell-fate specification, regulation of differentiation, andcontrol of cell proliferation and movement [45].Recently, we have shown that activation of the heat-shock response in the ciliate T. ... followed by 45cycles of denaturation at 95 °C for 30 s and annealing andextension at 60 °C for 15 s each. Following amplification,melting curve analysis of the DNA was performed at tem-peratures ... that facilitate template nucleation of microtu-bules by the c-tubulin ring complex at cold temperatures.AbbreviationsqPCR, quantitative PCR; RATE, rapid amplification of telomeric ends; TuRC, tubulin...