Báo cáo Y học: Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4 pdf

... genomic organization of MUC4 we determined by comparison of the published cDNA and the evolving human draft sequence [20] is very close to that of the mouse Muc4 (Table 1). The size of intron 1 of the human MUC4 ... member of the large membrane-bound mucins family. MUC4, SMC and Muc4 have a smaller cytoplasmic tail than the other members of the...

Ngày tải lên: 08/03/2014, 23:20

... extra copies of the argU, ileY ,andleuW tRNA genes. The coprecipitation of OraS and O raE might imply that (a) the apoenzyme or O raE is not properly folded; and (b) the noncovalent interaction between these ... both genes was cloned i nto the same expression vector under the control of the T7 promoter, and coexpression of oraE and oraS genes was carried out in E...

Ngày tải lên: 30/03/2014, 15:20

... ambp _human_ 2), human Alzheimer’s disease amyloid a4 protein precursor (a4 _human) and human type 1 and type 2 hepatocyte growth factor activator inhibitors (spt1 _human_ 1, spt1 _human_ 2, spt2 _human_ 1, ... °CÆh )1 . Effect of the recombinant protein on the activity of proteases The activity of the proteases on synthetic peptide-pNA substrates was monitored spectroph...

Ngày tải lên: 31/03/2014, 01:20

... igration and resolution medi- ated by the E. coli RuvABC resolvasome complex requires the binding of RuvA to one face of the junction and RuvC to the other [8,9]. Complexes formed by the loading of ... migration by RuvAB. This reaction may require a double tetramer of RuvA or the assembly of a RuvABC resolvasome to anchor the complex [18]. In the case of...

Ngày tải lên: 17/03/2014, 17:20

... also as a chaperone and help the proximal and distal parts of the long tail fibers to join correctly by increasing the effective target sizes and thereby increasing the rates of otherwise slow diffusion–limited ... of a helicity at this temperature and changes in the magnitude of the accompanying enthalpy. The ratio o f the van’t Hoff enthalpy to calorimetri...

Ngày tải lên: 24/03/2014, 03:21

... degree of polymerization. The mode of action and specificity as well as stereoselectivity of hydrolysis catalyzed by the exo- 1,3-b-glucanase were studied by NMR spectroscopy. MATERIALS AND METHODS Substrates Laminarin ... water. The purified products of hydrolysis were analyzed by 1 H and 13 C NMR spectroscopy as described previously [30]. Determination of the stereoche...

Ngày tải lên: 24/03/2014, 04:21

... ribozyme reactions and the polyanionic nature of RNA, the catalytic chem- istry and folding of group I ribozymes (and other large ribozymes) require divalent metals [11,12]. Whereas only Mg 2+ and ... 23S.5DG b pre-RNA, and the binding of a minimum of n Mg 2+ ions leads to formation of the active complex, whereas binding of a minimum of m Mn 2+ ions forms the...

Ngày tải lên: 19/02/2014, 07:20

... dynamic behavior of the minidomain may influence the state of the N-domain and may therefore modify the efficiency of the decod- ing process. To verify this hypothesis, we generated a series of ... one side of the b -sheet and two on the other side. The rmsd of the heavy atoms (Ca, C, and N) of the protein core, when the NMR structures of both confo...

Ngày tải lên: 06/03/2014, 11:20

... with the concentration of copper, as could be expected with a change in the hydrophobicity of the local environment of Tyr63 and/ or a contribution of the binding of the copper to the thiolates of ... a probe to monitor the binding of metal ions to the protein. Study of the binding of Cd(II) to EhCopZ by fluorescence spectroscopy, dynamic light scat...

Ngày tải lên: 23/03/2014, 12:20

... folding of p53 into an active form [22], we evaluated whether ATP has any influence on E2F1 inhibition by MDM2. A titration of MDM2 in the presence of ATP stimulated the inhibitory activity of MDM2 towards ... on another of its tar- get proteins, the transcription factor E2F1. In the absence of ATP, MDM2 was able to catalyse inhibition of the DNA-binding function of...

Ngày tải lên: 24/03/2014, 00:21