Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx

... verify our hypothesis, bovine cDNA encoding the 38-kDa protein was cloned to iden tify the catalytic activity of the expressed protein. Cloning of the cDNA encoding bovine sterol D14-reductase Bovine ... similarity with D14-SR from yeasts, fungi, and plants (55±59%), sug- gesting that the bovine cDNA encodes D14-SR. Northern blot analysis of bovine tissues showed hig...

Ngày tải lên: 08/03/2014, 16:20

... Biology (ZMBP), University of Tu ¨ bingen, Germany; 2 Department of Plant Biology, University of Valencia, Spain The aldo-keto reductase (AKR) superfamily comprises proteins that catalyse mainly ... superfamily [8]. The members of the AKR superfamily are cytosolic, monomeric proteins that catalyse mainly the NAD(P)H- dependent reduction of a wide variety of carbonyl com- pounds; s...

Ngày tải lên: 18/03/2014, 01:20

... vivo expression of IL-11 A range of tissues (head kidney, gills, liver, spleen, muscle, skin, intestine and brain) were collected from three healthy fish for analysis of constitutive expression of ... Regulation of IL-11 expression in intestinal myofibroblasts: role of c-Jun AP-1- and MAPK-dependent pathways. Am J Physiol Gastrointest Liver Physiol 285, G529–G538. 9 M...

Ngày tải lên: 07/03/2014, 16:20

... the physical role of MeJA is to mobilize JA [ 19]. The proof of this hypothesis might come from a more detailed understanding of how plants form MeJA from JA, and vice versa. A recent study identified ... acceptance and enzymatic activity of those enzymes, if known, is highly diverse. With the increasing number o f s pecified plant enzymes of this g roup of the a/b- hydrolas...

Ngày tải lên: 16/03/2014, 18:20

... contraction by activating ryanodine receptors of the sarcoplasmic Fig. 1. Isolation of tigrin from the Duvernoy’s glands of R. tigrinus tigrinus. (A) The extract from Duvernoy’s glands of R. tigrinus ... microliters of 4-vinylpyridine were then added, and alkylation was allowed to proceed for 3 h at room temperature. The S-pyridylethylated proteins were separated from the re...

Ngày tải lên: 18/03/2014, 01:20

... Hassan Y. Naim 2 and Tosso Leeb 1 1 Institute of Animal Breeding and Genetics and 2 Institute of Physiological Chemistry, School of Veterinary Medicine Hannover; 3 Institute of Molecular Biology, ... analyses and in the RT-PCRs, the two experiments clearly indicate a tissue-specific expression pattern of the NICN1 gene. Tissues with strong NICN1 expression are brain, tes...

Ngày tải lên: 23/03/2014, 21:20

... pathway, allows cells to activate monosaccharides that come from nutrition or from lysosomal degradation of glycoproteins and glycolipids. The sugars are phosphorylated by kinases and activated by ... analysis of the product of the enzymatic reaction catalyzed by GDP- L -fucose pyrophosphorylase. (A) Vector control in COS-7 cell lysate; (B) putative mouse pyrophos- phorylase in...

Ngày tải lên: 30/03/2014, 13:20

... height of the orbit and extends just above the top of the most-anterior ethmoid cells. By the age of 8 years, the top of the frontal sinuses is at the level of the orbital roof, and by the age of ... the age of 6 years. Their develop- ment is quite variable but effectively appears to start only after the second year of life [4,8,9]. By the age of 4 years, the averag...

Ngày tải lên: 25/10/2012, 11:04

... 4. Department of Statistics, University of Connecticut, Storrs, CT, USA 5. Department of Medicine, Division of Rheumatology, Allergy and Immunology, School of Medicine, University of California ... study, were not allowed. Treatment compliance Compliance was assessed by capsule count at visits 3, 4, and 5 and review of subject diary. Efficacy and Safety Variables...

Ngày tải lên: 26/10/2012, 09:48

... b b i i o o g g r r a a p p h h y y Jinlin Hou, MD, is Director and Professor of Hepatology Unit and Department of Infectious Diseases, Nanfang Hospital, Southern Medical University. Dr. Hou joined the University Department ... include clinical management of viral hepatitis, and molecular virology and immunology of HBV infection. Zhihua Liu, PhD, is lecturer of He...

Ngày tải lên: 02/11/2012, 11:12