Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx

... FEBS 2003 Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry Maria I. Klapa*, Juan-Carlos Aon† and Gregory Stephanopoulos Department of Chemical ... we present data validating the use of mass spectrometry (MS) for the quantification of complex metabolic flux networks. In the context of the...

Ngày tải lên: 08/03/2014, 08:20

... the IKK complex and activation of the clas- sical NF-jB pathway. Instead, we gain more and more evidence that recruitment, modification and acti- vation and inactivation of the E3 ligases and kinases involved ... Activation of the alterna- tive NF-jB pathway is independent of IKK2 and NEMO and requires degradation of TRAF proteins and subsequent activation of IKK1...

Ngày tải lên: 28/03/2014, 23:20

... 2. Intact-cell MALDI-TOF mass spectra of mycelia and spores of Trichoderma strains. The masses 7347 and 7494 of T. atroviride spores correlate with two proc- essed products of the spore hydrophobin SRH1 ... MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H. jecorina grown on malt agar. The masses displayed have an error of a...

Ngày tải lên: 19/02/2014, 02:20

... external and internal environment. Because of the multiplicity and broad substrate specificity of signaling enzymes, it is of immense importance to understand how the cell achieves efficiency and accuracy ... PDZ domains, and binds a variety of receptors ⁄ channels, cytoskeletal and Table 1. Scaffold proteins and their kin. Representatives of the four categories of scaff...

Ngày tải lên: 06/03/2014, 22:21

... was dissolved in 20 lLof8 M urea and incubated at 37 °C for 1 h. To the solution, 60 lL of 0.2 M Tris/HCl buffer (pH 9.0) and 5 pmol of lysyl endopeptidase were added, and the m ixture was incubated ... The enzyme was stable up to 60 °C in the presence of 40 m M D -aspartate (Fig. 2 A). We further examined the effects of the c oncen trations of L -and D -aspartate on th e...

Ngày tải lên: 07/03/2014, 16:20

... one of the most frequent words in the English language, accounting for about 1% of tokens in text and over a quarter of all third-person pronouns. 1 Usually between a quarter and a half of it ... pio- neering work of Paice and Husk (1987), the inclu- sion of non-referential it detection in a full anaphora resolution system by Lappin and Leass (1994), and the machine lear...

Ngày tải lên: 08/03/2014, 01:20

... the major bands at 22 and 42 kDa, there appeared minor bands at molecular masses of around 20 and 39– 41 kDa, which partly shifted from the major bands, coinciding with the appearance of a significant ... 179 theoretical isoelectric point and molecular mass of 9.11 and 63 129 Da, respectively, before removal of the pre- dicted signal peptide and 9.01 and 60 147 Da after r...

Ngày tải lên: 16/03/2014, 04:20

... cytosolic precursors of PSRP-2, PSRP-3, and PSRP-4 have average targeting peptides (62, 58, and 54 residues long), and the mature proteins are of 196, 121, and 47 residues length (molar masses, 21.7, 13.8 and ... amplifications of 3¢-portions of Psrp-2 (P2-1/ PR) and Psrp-3 (P3-1/PR), and 50–57 °C for amplification of 3¢-portion of Psrp-4 (P4-1/PR). Here, for example,...

Ngày tải lên: 17/03/2014, 09:20

... University of Amsterdam, the Netherlands 3 Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, the Netherlands Recently, identification of the lithotroph ... 2D 1 H- 13 C COSY the number of complex points and sweep widths were 2000 points ⁄ 6 p.p.m. for 1 H and 512 points ⁄ 150 p.p.m. for 13 C. In the 2D 1 H- 1 H COSY the number...

Ngày tải lên: 23/03/2014, 15:20

... label of o and its observed attributes; (2) the cor- relations between the label of o and the observed at- tributes and labels of nodes connected to o; and (3) the correlations between the label of ... satire classification of news articles (Burfoot and Baldwin, 2009). This and other work has established the use- fulness of document classifiers as stand-alone sys- tems...

Ngày tải lên: 23/03/2014, 16:20