Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
... FEBS 2003 Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry Maria I. Klapa*, Juan-Carlos Aon† and Gregory StephanopoulosDepartment of Chemical ... wepresent data validating the use of mass spectrometry (MS)for the quantification of complex metabolic flux networks. In the context of the lysine biosynthesis flux network of Corynebacterium glutamicum ... analysis of undetermined metabolic networks: the quest for the missingconstraints. TIBTECH 15, 308–314.7. Klapa, M.I. (2001) High resolution metabolic flux quantification using stable isotopes and mass...
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Báo cáo khoa học: TNFR1-induced activation of the classical NF-jB pathway Harald Wajant1 and Peter Scheurich2 pptx
... the IKK complex and activation of the clas-sical NF-jB pathway. Instead, we gain more and more evidence that recruitment, modification and acti-vation and inactivation of the E3 ligases and kinasesinvolved ... Activation of the alterna-tive NF-jB pathway is independent of IKK2 and NEMO and requires degradation of TRAF proteins and subsequent activation of IKK1 [1,2]. IKK1 phos-phorylates p100 and thereby ... kinase activity of the IKK complex and prote-olytic degradation of IjB proteins. The alternative NF-jB pathway is activated by a limited subgroup of TNF ligands and involves activation of NIK-mediated...
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Tài liệu Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx
... 2. Intact-cell MALDI-TOF mass spectra of mycelia and spores of Trichodermastrains. The masses 7347 and 7494 of T. atroviride spores correlate with two proc-essed products of the spore hydrophobinSRH1 ... MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H. jecorina grown on maltagar. The masses displayed have an error of about 0.1%, so peaks of 7232 ... server(http://www.expasy.org/) or the peptide mass calculator(http://rna.rega.kuleuven.ac.be/masspec/pepcalc.htm). Tocorrelate observed mass peak data, masses of variousN- and C-terminally processed and oxidized peptides...
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Báo cáo khoa học: Functional classification of scaffold proteins and related molecules pptx
... external and internal environment. Because of the multiplicity and broad substrate specificity of signaling enzymes, itis of immense importance to understand how the cellachieves efficiency and accuracy ... PDZ domains, and bindsa variety of receptors ⁄ channels, cytoskeletal and Table 1. Scaffold proteins and their kin. Representatives of the four categories of scaffold proteins and their relatives ... several attributes of scaffold function, such as the ability of binding multi-ple partners, mediating their complex and transientinteractions and themselves undergoing a complex array of regulatory...
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Báo cáo khoa học: Molecular identification of monomeric aspartate racemase from Bifidobacterium bifidum pptx
... was dissolved in 20 lLof8Murea and incubated at 37 °C for 1 h. To the solution, 60 lL of 0.2MTris/HCl buffer (pH 9.0) and 5 pmol of lysylendopeptidase were added, and the m ixture was incubated ... Theenzyme was stable up to 60 °C in the presence of 40 mMD-aspartate (Fig. 2 A). We further examined the effects of the c oncen trations of L -and D-aspartate on th e e nz ymestability and found ... One unit of the enzyme was defined as theamount of enzyme that catalyzes the formation of 1 lmol of D-aspartate fromL-aspartate p er hour.Protein concentrations were determined using a proteinassay...
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Báo cáo khoa học: "Distributional Identification of Non-Referential Pronouns" pdf
... one of the most frequent words inthe English language, accounting for about 1% of tokens in text and over a quarter of all third-personpronouns.1Usually between a quarter and a half of it ... pio-neering work of Paice and Husk (1987), the inclu-sion of non-referential it detection in a full anaphoraresolution system by Lappin and Leass (1994), and the machine learning approach of Evans ... the length of the base forms of words in that language. For real-world application of our approach, truncation alsoreduces the table sizes (and thus storage and look-up costs) of any pre-compiled...
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Báo cáo khoa học: The identification of a phospholipase B precursor in human neutrophils doc
... themajor bands at 22 and 42 kDa, there appeared minorbands at molecular masses of around 20 and 39–41 kDa, which partly shifted from the major bands,coinciding with the appearance of a significant ... 179theoretical isoelectric point and molecular mass of 9.11 and 63 129 Da, respectively, before removal of the pre-dicted signal peptide and 9.01 and 60 147 Da afterremoval of the predicted signal peptide. ... was ruled out bythe absence of bacterial DNA. Characterization of Table 1. Peptide mass fingerprint of the 22 and 42 kDa fragments. The 22 and 42 kDa bands on Coomassie Brilliant Blue-stained...
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Báo cáo khoa học: Proteomic identification of all plastid-specific ribosomal proteins in higher plant chloroplast 30S ribosomal subunit PSRP-2 (U1A-type domains), PSRP-3a/b (ycf65 homologue) and PSRP-4 (Thx homologue) doc
... cytosolicprecursors of PSRP-2, PSRP-3, and PSRP-4 have averagetargeting peptides (62, 58, and 54 residues long), and themature proteins are of 196, 121, and 47 residues length(molar masses, 21.7, 13.8 and ... amplifications of 3¢-portions of Psrp-2 (P2-1/PR) and Psrp-3 (P3-1/PR), and 50–57 °C for amplification of 3¢-portion of Psrp-4 (P4-1/PR). Here, for example, P2-1/PR stands for PCR amplified DNA using ... library using the tagged primers.The nucleotide sequences of Psrp-2 and Psrp-4 wereobtained by sequencing P2-3/P2-4 and P4-3/P4-4 using sequencing primers, TAG1 and TAG2, and the two strandswere...
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Báo cáo khoa học: Structural identification of ladderane and other membrane lipids of planctomycetes capable of anaerobic ammonium oxidation (anammox) pptx
... University of Amsterdam, the Netherlands3 Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, the NetherlandsRecently, identification of the lithotroph ... 2D1H-13CCOSY the number of complex points and sweep widthswere 2000 points ⁄ 6 p.p.m. for1H and 512points ⁄ 150 p.p.m. for13C. In the 2D1H-1H COSY thenumber of complex points and sweep widths ... modification of theladderane lipids.BAFig. 8. Gas chromatograms of (A) the TLF of a 99.5% pure suspension of Candidatus‘B. anammoxidans’ strain Dokhaven, and (B)the TLF after acid hydrolysis of the...
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Báo cáo khoa học: "Collective Classification of Congressional Floor-Debate Transcripts" ppt
... label of o and its observed attributes; (2) the cor-relations between the label of o and the observed at-tributes and labels of nodes connected to o; and (3)the correlations between the label of ... satireclassification of news articles (Burfoot and Baldwin,2009). This and other work has established the use-fulness of document classifiers as stand-alone sys-tems and as components of broader NLP ... Examples of other applications are dependency parsing (Smith and Eisner, 2008) and alignment (Cromires and Kurohashi, 2009). Conditional random fields(CRFs) are an approach based on Markov randomfields...
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