Báo cáo khoa học: Plasminogen activator inhibitor type-1 inhibits insulin signaling by competing with avb3 integrin for vitronectin binding pptx

... PRIORITY PAPER Plasminogen activator inhibitor type-1 inhibits insulin signaling by competing with avb3 integrin for vitronectin binding Roser Lo ´ pez-Alemany 1 , Juan ... between integrins and growth factor receptors has been reported for several systems, one of which is the modulation of insulin signaling by avb3 integrin. Plasminogen activator...

Ngày tải lên: 08/03/2014, 08:20

... by reducing the binding affinity for substrate of residues Glu168 and Asn189. These results suggest that ATB107 is a novel potent inhibitor of IGPS, and that IGPS might be a potential target for ... high-affinity inhibitor, ATB107, of mIGPS, using the virtual screening approach. The inhibitor was found to be a competi- tive inhibitor of mIGPS, as it reduced the binding affinity...

Ngày tải lên: 07/03/2014, 03:20

... order was established, with the cofactor binding first and sulfite binding second. The low dissociation con- stant for the cofactor product NADPH combined with the reduced values for D V and k cat implies ... and D V ⁄ K for both substrates as presented in Table 2. The D V value for PTDH-E175A ⁄ A176R was 2.21 ± 0.03 with NAD and 1.7 ± 0.1 with NADP. Therefore, the hydride t...

Ngày tải lên: 23/03/2014, 15:20

... be consistent with such a model. Formation of TGase isoforms at distinct differentiation stages is being investigated. Acknowledgements This work was supported by the Deutsche Forschungsgemeinschaft ... °Cfor30minwith20lL (1 pmol) TAMEP, 500 lL (20 U) immobilized chymotryp- sin or 250 lL(5U)immobilizedtrypsinin50m M Tris/HCl, pH 7.0. Immobilized proteases were removed by centrifu- gatio...

Ngày tải lên: 31/03/2014, 07:20

... was 37 lm for the D31A mutant and 198 lm for the D31L mutant, as compared to 0.70 lm for p807. This corres- ponds to a 51-fold increase for the D31A mutant and a 283-fold increase for the D31L ... enzyme assay for determining functionality of the DHFR mutant forms was performed as described previ- ously [4,5]. Each enzyme sample was measured multiple times. A control reaction with...

Ngày tải lên: 16/03/2014, 10:20

... X-100 for 5 min, rinsed once in NaCl/P i then incubated with 0.1% (w/v) NaBH 4 for 5 min prior to three w ashes with NaCl/P i . Non- specific binding was blocked by adding 1% BSA in NaCl/P i for ... for 8 h (middle panels) or incubated for 1 h with 20 l M SB203580 then 8 h with 50 l M MG132 ( lower panels) before being processed as in Fig. 4. The localization of the endog...

Ngày tải lên: 23/03/2014, 12:20

... of S.W. was supported by the Deutsche Forschungsgemeinschaft (We2843 ⁄ 2-1) and BGAG-Stiftung Walter Hesselbach. The work of S.B. is supported by grants from the Deut- sche Forschungsgemeinschaft ... CagL, CagY and CagA, which by itself binds the host cell integrin member b 1 followed by delivery of CagA across the host cell membrane. A role of CagA interaction with phosphatidylse...

Ngày tải lên: 14/02/2014, 19:20

... fixed with 3.7% paraformal- dehyde, quenched with 50 mm ammonium chloride for 15 min, and permeabilized with 0.1% Triton X-100 for 4 min, before being treated with 1.5 m hydrochloric acid for 10 ... detected by Nile red stain- ing as previously described [42]. Cell imaging for Nile red staining was performed by confocal microscopy. Quantifica- tion of lipid droplets was perf...

Ngày tải lên: 14/02/2014, 22:20

... first incubated for 24 h in 100 mm KCl to allow for quad- ruplex formation and then with GST-tag-digested EWS for 1 h at room temperature. The EWS–DNA complexes were resolved by 6% PAGE and visualized by ... G-quadruplex binding specificity by – – EWS EWS ssDNA L ETS-1 ssDNA S Htelo dsHtelo BA – – EWS EWS – EWS Fig. 1. Affinity of EWS for binding to G-quadruplex DNA. (A) EMSA...

Ngày tải lên: 15/02/2014, 01:20

... (A,B) BY4 742 ath1D trans- formed with pGFP–ATH1 under the MET25 promoter in YN glucose without methionine. (A) (left) fluorescence, (right) DIC. (B) Immu- noblot with anti-GFP of crude extract before ()) ... crude extract before ()) and after (+) deglycosylation with EndoH using DsRed polyclonal antibody (C). (D) BY4 741 cells transformed with pSPPEP4–ath1DN– mCherry were collected...

Ngày tải lên: 18/02/2014, 06:20