Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx

... 1814–1818. 634 P. Chopra et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis Puneet Chopra 1,2 , Anubha Singh 1,3 , Anil ... (H117Q) resulted in loss of both ATP binding and hydrolysis activity (Fig. 3). Thus, H117 is crucial for ATPase activity. Secretion of nucleoside diphosphat...

Ngày tải lên: 08/03/2014, 08:20

... mechanisms of calpain 7 in vitro: (a) by binding of MIM, MIT domains dissociate from the protease domain to expose the catalytic core; and (b) binding of MIM causes a conformational change of calpain ... the C-terminal half of PacC [16]. A human ortholog of PalA, ALIX (also known as AIP1), functions in the budding of enveloped RNA viruses from plasma Y. Osako et al. Enhancem...

Ngày tải lên: 18/02/2014, 04:20

... expected size of a DNA fragment amplified from the cDNA was 378 bp, whereas the size of a DNA fragment amplified from genomic Xantha-g DNA was 566bp.DNAfragmentsof378bpcouldbeisolatedfrom the wild-type ... encoding a third subunit, analysis of the interaction of the three subunits with the yeast two-hybrid system, and recon- stitution of the enzyme activity by co-expression of...

Ngày tải lên: 19/02/2014, 12:20

... the mechanism of action of chymotrypsin: the role of the low barrier hydrogen bond. Biochemistry 36, 4576–4584. 46. Warshel, A. (1998) Electrostatic origin of the catalytic power of enzymes and the role of ... results from the strong polarization of the C–O bond by the dipoles of the oxyanion hole in conjunction with the influence of a close nucleophile like the Oc of the...

Ngày tải lên: 20/02/2014, 23:20

... of its chaper- one activity has been performed. Wheat TaHsp17.8C-II is  33% identical overall to TaHsp16.9C-I. Only two previ- ous studies have examined the chaperone activity of this classofsHsp,andnoplantclassIIsHsphasbeentested for ... the ratio of sHsp to substrate that is required to suppress light scattering can be used as a measure of effectiveness of the chaperone. Usin...

Ngày tải lên: 07/03/2014, 15:20

... inhibited growth of the test bacterium M. luteus at a c oncentration of 4.5 l M . Histone H2A from a variety of v ertebrate species has been shown to poss ess an timicrobial activity, but its activity appears ... the results of a proteomic investigation of hemocyte antimicrobial peptides of the P acific white shrimp, L. vannamei, and the r ole of histones as potentially impo...

Ngày tải lên: 07/03/2014, 16:20

... surface of the molecule and is rather far away from the trinuclear site. Therefore, the C-terminal sequence of RlL might be more of an evolu- tionary relic than a functional feature of the enzyme. The ... at pH levels ranging from 2 to 8. Acknowledgements We are grateful to M. Paloheimo from Roal Oy for providing us with samples of TaLcc1. The ESRF is thanked for provision of...

Ngày tải lên: 14/02/2014, 18:20

... Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane. The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, from ... conformation around the active site of ApeSOD is independent of the presence of a metal cofactor. Figure 3D shows the superimposition of the active site structures of apo,...

Ngày tải lên: 14/02/2014, 22:20

... wide range of spectroscopic techniques. Suitable methods for investigation of the secondary structure and morphology of such structures, however, require the presence of large amounts of the oligomeric state, ... distribu- tion of 100 l M proinsulin C-peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C-peptide in the presence...

Ngày tải lên: 18/02/2014, 04:20

... prior studies of nonheme Fe 2+ centers [29–31], is assigned to the mixing of the 4p orbital with the 3d orbital of the metal cofactor. Two important pieces of information can be gleaned from the pre-edge ... aliquots of 35 lm or 70 lm from a 3.7 mm stock solution); xanthine (360 lm) in the presence of 2-ketoglutarate (1 mm); catechol (200 lm); and quercetin (170 lm). The durat...

Ngày tải lên: 18/02/2014, 06:20