Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx
Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx
... 1814–1818.
634 P. Chopra et al. (Eur. J. Biochem. 270) Ó FEBS 2003
Cytotoxic activity of nucleoside diphosphate kinase secreted
from
Mycobacterium tuberculosis
Puneet Chopra
1,2
, Anubha Singh
1,3
, Anil ... (H117Q)
resulted in loss of both ATP binding and hydrolysis activity
(Fig. 3). Thus, H117 is crucial for ATPase activity.
Secretion of nucleoside diphosphat...
Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
... mechanisms of calpain 7 in vitro:
(a) by binding of MIM, MIT domains dissociate from
the protease domain to expose the catalytic core; and
(b) binding of MIM causes a conformational change
of calpain ... the C-terminal half of PacC [16]. A human ortholog
of PalA, ALIX (also known as AIP1), functions in the
budding of enveloped RNA viruses from plasma
Y. Osako et al. Enhancem...
Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt
... expected size of a DNA fragment amplified
from the cDNA was 378 bp, whereas the size of a DNA
fragment amplified from genomic Xantha-g DNA was
566bp.DNAfragmentsof378bpcouldbeisolatedfrom
the wild-type ... encoding a third subunit, analysis of the interaction of
the three subunits with the yeast two-hybrid system, and recon-
stitution of the enzyme activity by co-expression of...
Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... the
mechanism of action of chymotrypsin: the role of the low barrier
hydrogen bond. Biochemistry 36, 4576–4584.
46. Warshel, A. (1998) Electrostatic origin of the catalytic power of
enzymes and the role of ... results from
the strong polarization of the C–O bond by the dipoles of
the oxyanion hole in conjunction with the influence of a
close nucleophile like the Oc of the...
Báo cáo khóa học: Chaperone activity of cytosolic small heat shock proteins from wheat pptx
... of its chaper-
one activity has been performed. Wheat TaHsp17.8C-II is
33% identical overall to TaHsp16.9C-I. Only two previ-
ous studies have examined the chaperone activity of this
classofsHsp,andnoplantclassIIsHsphasbeentested
for ... the ratio of sHsp to substrate that is required to
suppress light scattering can be used as a measure of
effectiveness of the chaperone. Usin...
Báo cáo khoa học: Antimicrobial activity of histones from hemocytes of the Pacific white shrimp ppt
... inhibited growth
of the test bacterium M. luteus at a c oncentration of 4.5 l
M
.
Histone H2A from a variety of v ertebrate species has been
shown to poss ess an timicrobial activity, but its activity
appears ... the results of a proteomic investigation
of hemocyte antimicrobial peptides of the P acific white
shrimp, L. vannamei, and the r ole of histones as potentially
impo...
Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... surface of the molecule and is rather
far away from the trinuclear site. Therefore, the
C-terminal sequence of RlL might be more of an evolu-
tionary relic than a functional feature of the enzyme.
The ... at pH levels ranging from 2 to 8.
Acknowledgements
We are grateful to M. Paloheimo from Roal Oy for
providing us with samples of TaLcc1. The ESRF is
thanked for provision of...
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... Three of the ligands,
OD2 of Asp165, NE2 of His79 and NE2 of His169,
formed an equatorial plane. The other protein ligand,
NE2 of His31, bound to the metal, in the company of
a water oxygen, from ... conformation
around the active site of ApeSOD is independent of
the presence of a metal cofactor.
Figure 3D shows the superimposition of the active
site structures of apo,...
Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx
... wide
range of spectroscopic techniques.
Suitable methods for investigation of the secondary
structure and morphology of such structures, however,
require the presence of large amounts of the oligomeric
state, ... distribu-
tion of 100 l
M proinsulin C-peptide in the
presence of divalent Ca
2+
and Mg
2+
ions
under native conditions (B), and of 100 l
M
C-peptide in the presence...
Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... prior studies of nonheme
Fe
2+
centers [29–31], is assigned to the mixing of the
4p orbital with the 3d orbital of the metal cofactor.
Two important pieces of information can be gleaned
from the pre-edge ... aliquots of 35 lm or 70 lm from a
3.7 mm stock solution); xanthine (360 lm) in the presence
of 2-ketoglutarate (1 mm); catechol (200 lm); and quercetin
(170 lm). The durat...
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