Báo cáo khoa học: Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase pot

... Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase Nicola Solaroli 1,2 , Mia Bjerke 1 , Marjan H. Amiri 1 , ... multisubstrate deoxyribonucleoside kinase of Droso- phila melanogaster (Dm-dNK) is sequence-related to three human deoxyribonucleoside kinases and to herpes simplex virus type-1 thymidine kinase. Dm-dNK ... Dm-dNK,...

Ngày tải lên: 08/03/2014, 02:21

... requirements of the dfp-707 and dfp-1 mutants correspond to those of panD mutants, however, dfp -mutants contained wild-type levels of aspartate decarboxylase [14], which is required for synthesis of b-alanine. ... important for the second-half reaction and are part of the active- site of CoaB; these residues may be directly involved in binding of one of the substrates,...

Ngày tải lên: 19/02/2014, 12:20

... FEBS 1233 integration of kinetic studies with docking studies, and computation of the pK a values of the titratable resi- dues of the active site, suggest that the formation of the enzyme–substrate ... k c ) ⁄ k 1 ): hence, the independence of the K m of HEX from pH and that of the K m of CAD from tem- perature, and the strong dependence of k c values of both substrat...

Ngày tải lên: 06/03/2014, 00:21

... indicative of an end -of- move boundary. It is evident from the language model that pause plays an important role in the prediction of continuers. A quarter of all relevant trigrams consist of a part -of- speech ... necessarily in consecutive order. There are seven occurrences of P(<bc> ) with a back-off value of one. This shows the result of the forced back-off after a...

Ngày tải lên: 22/02/2014, 02:20

... viable 5¢ and 3¢ splice sites (5¢ss and 3¢ss) at either end]. Presently, there is evidence that inclusion of many of these sequences is actively inhibited due to the presence of intrinsic defects ... presence of silencer elements [29–31] or the formation of inhibiting RNA secondary structures [32]. Even if a combination of all these ele- ments succeeds in repressing the use of m...

Ngày tải lên: 06/03/2014, 09:22

... mg of [2- 13 C 1 ]- or [3- 13 C 1 ]glucose, 3.5 mg of phosphoenol pyruvate, 0.5 mg of ATP, 6 mg of 2-oxoglutaric acid, 0.6 mg of NAD + , 2.5 mg of NH 4 Cl, 5mgofa 13 C-isotopologue mixture of ... biotransformation of [2- 13 C 1 ]- or [3- 13 C 1 ]glucose, 5 units of hexokinase, 5 units of pyruvate kinase, 5 units of glucose 6-phosphate dehydrogenase, 5 units of glutama...

Ngày tải lên: 07/03/2014, 05:20

... Several lines of evidence support this hypothesis: (a) extrapolation of calorimetric data by Privalov [32], in his review on calorimetry in 1979, showed that the specific entropy of unfolding of several ... validity of this criterion has often been argued and therefore care should be taken when it is applied [20]. In the case of LamA, the DH cal ⁄ DH vH ratio deviates from unity, y...

Ngày tải lên: 07/03/2014, 05:20

... Character- ization of the reactions and purification of two forms of ketopantoyl lactone reductase. J Biol Chem 249, 4689– 4695. 28 Nazi I, Koteva KP & Wright GD (2004) One -pot chemoenzymatic preparation of ... acceleration of MM2281-catalyzed pantothenate synthesis by the removal of pantothenate is very prob- ably not due to a shift in the equilibrium of the reac- tion, le...

Ngày tải lên: 07/03/2014, 05:20

... categories are stripped off (Figure 2). While most of the state -of- the-art parsers are based on probabilistic CFGs, the parameteriza- tion of the probabilistic model of each parser varies. In ... (precision/recall/f-score) potential of a parser. Effectiveness of the parser en- semble is also attested by the fact that it resulted in larger improvements. Further investigation of t...

Ngày tải lên: 08/03/2014, 01:20

... Alan Wallington School of Computer Science, Univ. of Birmingham B15 2TT Birmingham, UK r.agerri@cs.bham.ac.uk Abstract In this paper we provide a formalization of a set of default rules that we ... means of depriving him of a necessity (food) which means that being deprived causes Connors’s defeat. This fits well with the interpretation of (1) where McEnroe’s playing deprived Con...

Ngày tải lên: 08/03/2014, 03:20