Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx

... N-terminal region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o f BMAL1 in a mammalian two-hybrid assay. Accordingly, a Fig. 5. Analysis of DEC1 mutants for binding to ... C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions...

Ngày tải lên: 19/02/2014, 16:20

... FEBS 4505 Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from ... fibronectin. The C-terminal domain of fibronectin-binding protein A is organized into 11 tandem repeats, six of which bind the ligand with high affinity; other sites bind more weakly. Fibr...

Ngày tải lên: 06/03/2014, 22:21

... 2A). In some cells, the mutant proteins were also observed in the cytoplasm, as seen for the wild-type protein. Similar staining patterns were observed in other human cell lines, including hepatoma ... the presence and absence of 0.25 m M galactose was determined by measuring BrdU incorporation. The level of BrdU incorporation into each cell line cultured in the absence of gal...

Ngày tải lên: 07/03/2014, 00:20

... the control of the GAL10 pro- moter. The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the expression of the OR gene ... for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. J Biotechnol 52, 127– 133. Expression of olfactory receptors in yeast for...

Ngày tải lên: 07/03/2014, 16:20

... region. The results obtained indicate that, although the substrate-binding site is located in the C-terminal domain, the E. coli Pta N-terminal domain is involved in stabilization of the hexameric native ... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig. 2). P...

Ngày tải lên: 06/03/2014, 11:20

... Regardless of dopamine treatment, overexpres- sion of a-synuclein significantly affected the levels of four proteins [stathmin 1, glutathione S-transferase (GST)p, Ran 1 binding protein and C1q binding ... suggested to be linked to early stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actin bundles regulator fascin 1 [27] and discord...

Ngày tải lên: 15/02/2014, 01:20

... concentration of the 16 kDa protein subunit) increased linearly with increasing fractional saturation of the metal site in Bxe_A2876, the k cat determined from the molar concentration of Fe 2+ -containing ... one of the following substrates was offered to a protein solution containing 7 lm metal sites: xanthine in the presence of 2-ketoglutarate; cat- echol; or quercetin. Howeve...

Ngày tải lên: 18/02/2014, 06:20

... kinetic analysis of these inhibiting or activating effects was either incomplete [22,36–39] or debatable [33,42]. In particular, reported results were interpreted in terms of an AAA site distinct ... 82 in binding of succinyldithiocho- line to human butyrylcholinesterase. Biochemistry 36, 2266–2277. 45 Masson P, Adkins S, Gouet P & Lockridge O (1993) Recombinant human bu...

Ngày tải lên: 18/02/2014, 17:20

... quantities of protein util- izing the principle of protein–dye binding. Anal Biochem 72, 248–254. 22 Knecht W, Bergjohann U, Gonski S, Kirschbaum B & Loffler M (1996) Functional expression of a ... use in gene therapy. Abbreviations ACV, 9-(2-hydroxyethoxymethyl)-guanine; AraA, 9-(b- D-arabinofuranosyl)-adenine; AraC, 1-(b-D-arabinofuranosyl)-cytosine; AraT, 1-(b- D-arabinofurano...

Ngày tải lên: 19/02/2014, 02:20

... cen- ters are inhibited for Q B reduction by binding of a DCMU molecule in the Q B pocket; the remaining PSII centers are unaffected. However, a saturating concen- tration of DCMU would inhibit completely ... FEBS 4773 of Q A – in PSII centers that are affected by the nonsatu- rating concentration of inhibitor [7,8]. The E m of Q A is raised in the presence of DCMU in the...

Ngày tải lên: 19/02/2014, 05:20