Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... N-terminal region of DEC1, including thebHLH domain, interacted with the C-terminal r egion o fBMAL1 in a mammalian two-hybrid assay. Accordingly, aFig. 5. Analysis of DEC1 mutants for bindingto ... C-terminal region, including the PAS-Bdomain of BMAL1, is required for the binding to DEC1,Fig. 4. Identiﬁcation of the domain of D EC1that interacts with BMAL 1. Interactions of various DEC1 ... activity of DEC1.Determination of the binding domain of DEC1 to BMAL1The interaction of DEC1 and BMAL1 was previouslydemonstrated by a yeast two-hybrid assay [ 7], and thisinteraction may be involved...

Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the ﬁbronectin-binding adhesins ﬁbronectin-binding protein A and ﬁbronectin-binding protein B from Staphylococcus aureus pot

... FEBS 4505 Functional analysis of a murine monoclonal antibodyagainst the repetitive region of the ﬁbronectin-bindingadhesins ﬁbronectin-binding protein A andﬁbronectin-binding protein B from ... ﬁbronectin. TheC-terminal domain of ﬁbronectin-binding protein A is organized into 11tandem repeats, six of which bind the ligand with high afﬁnity; other sitesbind more weakly. Fibronectin-binding ... 2010)doi:10.1111/j.1742-4658.2010.07835.xFibronectin-binding proteins A and B are multifunctional LPXTG staphy-lococcal adhesins, comprising an N-terminal region that binds ﬁbrinogenand elastin, and a C-terminal domain that interacts...

Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf

... 2A). In some cells, the mutant proteins were alsoobserved in the cytoplasm, as seen for the wild-typeprotein. Similar staining patterns were observed in other human cell lines, including hepatoma ... the presence and absence of 0.25 mMgalactose was determined by measuring BrdU incorporation. Thelevel of BrdU incorporation into each cell line cultured in theabsence of galactose was set to ... measuring theintensity of the individual GALE and tubulin bands in the western blot using Scion Image (Scion Corporation,Frederick, MD, USA).Generation of stable cell lines and BrdUincorporation...

Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx

... the control of the GAL10 pro-moter. The induction of the yeast, by galactose containingmedia, results in overexpression of GAL4, in turn inducingan increase of the expression of the OR gene ... for increasing theyield of properly folded recombinant human gammainterferon from inclusion bodies. J Biotechnol 52, 127–133.Expression of olfactory receptors in yeast for screening J. Minic ... function of increasing amount of totalproteins in membrane fractions from I7 yeast strain induced at15 °C, and from yeast expressing SSTR2 as a control.J. Minic et al. Expression of olfactory...

Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx

... region. The results obtainedindicate that, although the substrate-binding site islocated in the C-terminal domain, the E. coli PtaN-terminal domain is involved in stabilization of thehexameric native ... containingthe last 326 amino acids in the case of Pta-F1, the last 352amino acids in the case of Pta-F2, and the last 470 aminoacids in the case of Pta-F3 (Fig. 2).PCR reactions performed in ... domain. Taking into account the importantrole of Ptas, the results obtained in the present work,dissecting the different domains forming E. coli Pta,will help in the future manipulation of...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... Regardless of dopamine treatment, overexpres-sion of a-synuclein signiﬁcantly affected the levels of four proteins [stathmin 1, glutathione S-transferase(GST)p, Ran 1 binding protein and C1q binding ... suggested to be linked toearly stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actinbundles regulator fascin 1 [27] and discordant changes of two calcium-dependent, ... Proteomic analysis of protein complexes in human SH-SY5Y neuroblastomacells by using blue-native gel electrophoresis: an increase in lamin A ⁄ C associated with heat shock protein 90 in response...

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... concentration of the 16 kDaprotein subunit) increased linearly with increasingfractional saturation of the metal site in Bxe_A2876,the kcatdetermined from the molar concentration of Fe2+-containing ... one of the following substrates wasoffered to a protein solution containing 7 lm metalsites: xanthine in the presence of 2-ketoglutarate; cat-echol; or quercetin. However, when a series of ... &Rao Z (2007) An insight into the mechanism of human cysteine dioxygenase. Key roles of the thioether-bondedtyrosine-cysteine cofactor. J Biol Chem 282, 3391–3402.b-Diketone-cleaving oxygenase...

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... kinetic analysis of these inhibitingor activating effects was either incomplete [22,36–39]or debatable [33,42]. In particular, reported resultswere interpreted in terms of an AAA site distinct ... 82 in binding of succinyldithiocho-line to human butyrylcholinesterase. Biochemistry 36,2266–2277.45 Masson P, Adkins S, Gouet P & Lockridge O (1993)Recombinant human butyrylcholinesterase ... observationsobtained (Table 1).The binding of tyramine to D70G, in the presence of o-NPA, is weaker (9.1-fold) than binding to thewild-type enzyme, and induces an activating effect ono-NPA...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... quantities of protein util-izing the principle of protein–dye binding. Anal Biochem72, 248–254.22 Knecht W, Bergjohann U, Gonski S, Kirschbaum B &Lofﬂer M (1996) Functional expression of a ... use in gene therapy.AbbreviationsACV, 9-(2-hydroxyethoxymethyl)-guanine; AraA, 9-(b-D-arabinofuranosyl)-adenine; AraC, 1-(b-D-arabinofuranosyl)-cytosine; AraT,1-(b-D-arabinofuranosyl)-thymine; ... comprising deoxy-guanosine kinase (dGK), deoxycytidine kinase (dCK),thymidine kinase 2 (TK2) and herpes simplex virustype 1 thymidine kinase (HSV1-TK) (Fig. 1) [3]. Thestructures of Dm-dNK, human...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... cen-ters are inhibited for QBreduction by binding of aDCMU molecule in the QBpocket; the remaining PSIIcenters are unaffected. However, a saturating concen-tration of DCMU would inhibit completely ... FEBS 4773 of QA– in PSII centers that are affected by the nonsatu-rating concentration of inhibitor [7,8]. The Em of QAisraised in the presence of DCMU in the QBpocket,making it energetically ... reduction of QA in DCMU-inhibitedPSII is accounted for by the O–J phase. Interestingly,this decrease of AJ–Iwas compensated for by theequivalent increase of AO–J, making the sum of contri-butions...

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