... hybridized with the probe from each of the digested DNA samples(Fig. 3). Because there are no HindIII, XbaI, and BamHIsites within the 720-bp cDNA, a single band observed in the Southern blot hybridization ... derived from the DNA sequenceflanking the GAL4 activating domain and the ADC1terminator region in the pPC86 vector, respectively, wereused to amplify the rice cDNA inserted into vector pPC86.PCR ... transcription facto r, named RAMY, that binds to the 20-bp O2S sequences of the regulatory region of the Amy2 gene promoter has been identified using the yeast one-hybrid system from a rice library. The full...