Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles pot
Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles pot
... The signature amidase from Sulfolobus solfataricus belongs to the CX 3 C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles Elisa ... experimental analysis of the activity of the mutant SsAH-K96R on aliphatic amides and nitriles strengthens this hypothesi...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: "Constituent-Based Morphological Parsing: A New Approach to the Problem of Word-Recognition" pdf
... needed. The potential non-isomorphism between phonological and syntactic structure is derived from the narrow bandwidth of the channel between the phonological and syntactic components of the ... prosody and the non- isomorphism of syntactic and phonological structure. We maintain that these are are central to the task of a morphological analyzer and...
Ngày tải lên: 08/03/2014, 18:20
Báo cáo khoa học: A new rice zinc-finger protein binds to the O2S box of the a-amylase gene promoter pptx
... hybridized with the probe from each of the digested DNA samples (Fig. 3). Because there are no HindIII, XbaI, and BamHI sites within the 720-bp cDNA, a single band observed in the Southern blot hybridization ... derived from the DNA sequence flanking the GAL4 activating domain and the ADC1 terminator region in the pPC86 vector, respectively, were used to amplify...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: "Mapping Concrete Entities from PAROLE-SIMPLE-CLIPS to ItalWordNet: Methodology and Results" potx
... basis of the correspondences established between the conceptual classes of both ontologies, in order to narrow the automatic mapping; 3) Human validation of the automatic mapping and storage of ... to an objective difference of granularity of the ontologies. The problems in ii) may be bypassed by climbing up the ontological hierarchy, identifying the...
Ngày tải lên: 17/03/2014, 04:20
Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf
... through the rest of exons 15, 16, 17, 18, 19 and 20. s-DAPK-1’s coding region stops at the 126th base pair of intron 20–21 of the DAPK-1 gene, and the 3¢-UTR extends through the middle of intron ... tissues, suggesting that mRNA from the locus is coordinately produced. Future work will be required to understand the regulation of the translation of these m...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Fatty acid desaturases from the microalga Thalassiosira pseudonana pptx
... candidates for enzymes involved in the synthesis of EPA and DHA [18]. However, rather surprisingly, the first of these genes to be functionally characterized was found to encode a cytochrome b5 fusion ... dis- covery of genes involved in PUFA biosynthesis follow- ing the completion of the genome sequence of the EPA and DHA producing diatom T. pseudonana [17]. Le...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Evidence for two different electron transfer pathways in the same enzyme, nitrate reductase A from Escherichia coli potx
... transport of electrons from the two quinol types to [Fe-S] clusters, molybdenum cofactor and nitrate. To clarify the role of the hemes we have made spectral and kinetic studies of the reduction of these ... distal to the site of fumarate reduction; the use of the quinol-binding site inhibitor HOQNO shows that the inhibitor blocks binding of MQH 2...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin pptx
... describe the cDNA cloning of MBTI, and the gene expression of a 35-residue peptide and its mutant both derived from the long chain of the Lys active fragment of MBTI. The total synthesis of a 16-residue ... PSP, the electrophoretic band above the band of GST comes from the PSP enzyme preparation itself; compared with the molecular mass of GST, Lys33...
Ngày tải lên: 15/03/2014, 09:20
Báo cáo khoa học: Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures potx
... website for each deposited structure. It may be a good habit to have a look at the atomic coordinate section of the PDB file to see the level of the B-factors of the atoms that are in the most important ... X-rays – the shape and sym- metry of the cell define the directions of the diffracted beams, and the locations of all atoms in the cell define...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Nitric oxide formation from the reaction of nitrite with carp and rabbit hemoglobin at intermediate oxygen saturations pdf
... Three millilitres of Hb was transferred to the tonometer and equilibrated for 1 h in the shaking tonometer to ensure full equilibration of the Hb to the gas atmosphere of the system. The tonometer was then ... when the for- mation of HbNO and metHb (both tending to assume the R conformation) had caused an allosteric T to R transition. Both the initia...
Ngày tải lên: 16/03/2014, 06:20