Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf
Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf
... cDNA encoding cis -prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis Kasem Asawatreratanakul 1,2 , Yuan-Wei Zhang 1, *, Dhirayos Wititsuwannakul 3 , Rapepun Wititsuwannakul 4 , Seiji ... temperature control of 30 s at 95 °C, 30 s at 50 °C and 1 min at 72 °C with a 5 min preheat at 95 °C and a 10 min final ex...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf
... recorded against a reference cuvette con- taining NADPH, EDTA and buffer only. Subsequently, insulin was added, and a stable nonenzymatic rate was recorded. Finally, PDI was added, and the total NADPH oxidation ... refolding mixture was acidified and immediately analyzed by RP-HPLC. The amounts of native and linear tx 3a were calculated from their elution peak areas. The dat...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Gene cloning, expression and characterization of avian cathelicidin orthologs, Cc-CATHs, fromCoturnix coturnix pdf
... 480 Cc-CATH1 actgtcccctcgctgccttccatccaataaa ggtctttgctggtaaaaaaaaaaaaaaaa 531 Cc-CATH2 TTG-CTGAg-gaataaa -ggggc gtgtg c-accaagc -a 517 Cc-CATH3 g tc a cc c aataaa -c-g ttca-gct 540 C c- CATH 1 aa a a ... 60 Cc-CATH3 60 Cc-CATH1 CCCCTGGATTACAACCAGGCTCTGGCCCAGGCTGTGGACTCCTACAACCAACGGCCCGAG 120 Cc-CATH2 T AGC CC G AT A 120 Cc-CATH3 C 120 Cc-CATH1 GTGCAGAATGCCTTCAGGCTGCTCAGCGCCGACCCCGAACC...
Ngày tải lên: 28/03/2014, 23:20
Báo cáo khoa học: Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis) ppt
... 90 GAG GCC ACC AAT gtgagtagga 614 tatttaacag TCT AGT TCT Asn125 (3) 5 175 CGT ATC CAG G gtgagtctgt 226 ccccacacag CT CAG CTG TCC Ala184 (1) 6 131 AAA TAC CGA CTG gtaaacccaa 79 atgataccag GAC CTG ... 143 AAC AAA GCA G gtatattcag 434 atgtttccag GT GAG AGA ATG Gly640 (1) 16 156 CCG CCC CTT T gtaagcaacc 139 tcacctaaag CC AAA GCA GTG Ser693 (1) 17 52 GTC GTG CCA GA gtaagtgaca 368 ccgtgcacag G ......
Ngày tải lên: 31/03/2014, 07:20
Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf
... body (WB) at day 4 in the last larval instar, in the INT at day 0, 2, 4, 6 and 8 in the last larval instar, and in the adult male WB (#), female WB ($), testis TES and ovary (OVA), and L. decemlineata ... CTCCCGGCAAGCGTAAGACAAATC 3¢-RACE LdEcR-RF1 CCGTAGGAATGAGGGCAGAGTGTGT LdUSP-RF1 GATTTGTCTTACGCTTGCCGGGAG LdEcR-RF2 CATTCATCGTCTCGTGTATTTCCAG LdUSP-RF2 GACAAGCGACAAACAGATGCC T....
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Neuropeptide Y expression and function during osteoblast differentiation – insights from transthyretin knockout mice potx
... 5¢-GT AATGATCAGTCAACGGGGGAC-3¢ and 5¢-CCAGCA AGCTTGCAACCTTAACCA-3¢; for GAPDH, 5¢-ACTCC ACTCACGGCAAATTC-3¢ and 5¢-CCTTCCACAATGC CAAAGTT-3¢; for NPY, 5¢-TGGACTGACCCTCGCTC TAT-3¢ and 5¢-GATGAGGGTGGAAACTTGGA-3¢; ... 5¢-CCTGG GGTCACACCTAAAGA-3¢ and 5¢-TGTAAGGACACAC CGGAACA-3¢; for Y1 receptor, 5¢-CTCGCTGGTTCTCA TCGCTGTGGAACGG-3¢ and 5¢-GCGAATGTATATCT TGAAGTAG-3¢ [15]; for Y2 receptor, 5...
Ngày tải lên: 15/03/2014, 09:20
Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
... uences. Primer name Sequence 21 GGGTCTAGAATGGAAAAAGATCTACAGTTAAGA 33 CCGGAATTCTTATTTCCCTTCTCTCATCTC 40 GCGCGCCATGGAAAAAGATCTACAGTTAAGA 41 GGGGGATCCCCATAATCCACTCCACCTGCTAAA 44 GGGGGGGATCCT CATTATTTCCCTTCT 66 ... AATACCGCCATGTATAATATCTATTACTTC 67 GTAATAGATATTATACATGGCGGTATTGAA 75 GGGGGGGCCATGGAAAGAGCAGACGATTT 4294 H P. Chen et al.(Eur. J. Biochem. 271) Ó FEBS 2004 assay k it was obtained f rom...
Ngày tải lên: 30/03/2014, 15:20
Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
... proteins carrying a functional FLAG epitope from an excess of FLAG-negative com- petitors and from a large library of random variants was also undertaken by plasmid display, to confirm the efficacy of ... comparing the far-UV and near-UV CD spectra of PRAI and FLAG-PRAI in a buffer in which PRAI retains catalytic activity (Fig. 3). The spectra are effectively superim...
Ngày tải lên: 30/03/2014, 20:20
Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt
... frag- ments containing a complete x-5 gliadin gene, oligonucleo- tides, 5 ¢-AAGTGAGCAATAGTAAACACAAATCAAAC-3¢ and 5¢-CGTTACATTATGCTCCATTGACTAACAACGA TG-3¢, were constructed based on fragment DNA ... Foster City, CA, USA). Expression and purification of recombinant protein Sense (5¢-ATTTCATATGCAACAACAATTCCCCCAGC AACAATCA-3¢) and antisense (5¢-TCTCGGATCCTCA TAGGCCACTGATACTTATAACGTCGCT...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
... MW.Custom- made software and hardware were used for acquisition and analysis of data. Leakage and linear capacity currents were subtracted by using P/4 protocols. Data were sampled once every 5 ls and ... from cDNA libraries of adrenal gland, brain and fetal brain. BLAST searches of the human genomic sequence with the cDNA encoding the rat b 1A subunit C terminus als...
Ngày tải lên: 16/03/2014, 23:20