Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

... study by showing the direct interaction of Utp25p with SSU pro- cessome subunits, and the analysis of the 35S pre-rRNA cleavage reactions that are affected by the depletion of Utp25p. Furthermore, ... reactions of the pre-rRNA, among them the endonuclease MRP (responsible for the cleavage at site A 3 in ITS1 ) [3], the box C ⁄ D snoRNPs U3 and U14, and th...

Ngày tải lên: 22/03/2014, 21:21

... DONG NAM A NHfiNG NAM DAU THE KY XXI ThS NGUYEN THIHOAN Phien hgp todn the Diin ddn khu vuc ASEAN ldn thUlV, tqi Hd Ngi, ngdy 23-7-2010 B udc sang the ky XXI, nhan thflc dung ... xfl ly cac tinh hudng cu the& quot;'°. Tren thyc te, nudc ta da khai thdc dugc nhieu ngudn lyc, tao dugc the manh cho chien lugc bdo ve To quoc. Cd the coi chien l...

Ngày tải lên: 15/03/2014, 14:20

... Tropel and J. R. van der Meer 1766 FEBS Journal 272 (2005) 1756–1766 ª 2005 FEBS Characterization of HbpR binding by site- directed mutagenesis of its DNA -binding site and by deletion of the effector ... contacts the DNA. The objectives of this work were to identify critical motifs in the binding site for HbpR and to examine the necessity...

Ngày tải lên: 07/03/2014, 17:20

... for the CD11a and CD11b I domains exist because of the residual binding of the ICAM-4 negative cells. The mAb 60.1 reacting with the CD11b I domain almost completely inhibited the binding of both ... from the ligand binding site on the opposite side of the metal ion-dependent adhesion site (MIDAS) motif, whereas the binding interface for mAb 107 see...

Ngày tải lên: 20/02/2014, 11:20

... concentration of the samples, the protein concentration was determined by the Lowry assay and samples were further analysed by SDS ⁄ PAGE and UV ⁄ Vis spectroscopy. Purification of cytochrome c oxidase and ... oxidases and share both structural and functional homologies. Sub- unit I, as the core of the enzyme, houses two heme a moieties (heme a and a 3 ) and a...

Ngày tải lên: 06/03/2014, 00:21

... Under these conditions, the specific radioactivity of the 125 I-labelled PA1b, calculated by the ratio of the radioactivity measured by gamma counting and the amount of peptide evaluated by absorbance ... microsomal fraction of an extract of the resistant insect D. melanogaster also displayed a strong binding capacity. The determination of the characterist...

Ngày tải lên: 08/03/2014, 02:21

... (2002) Aminopeptidase N isoforms from the midgut of Bombyx mori and Plutella xylostella: their classification and the factors that deter- mine their binding specificity of Bacillus thuringiensis Cry1Ac ... con- stant against log of EDTA concentration. n, the slope of the plot, was calculated as 0.44 and estimates the number of molecules of EDTA needed to inactivate...

Ngày tải lên: 16/03/2014, 12:20

... that the GAL4–DBD fusion of MET sits on the UAS of the reporter construct even in the absence of ligand, and that the MET moiety is respon- sible for the observed repression. In the case of verteb- rate ... (Promega); the cDNA fragment containing the fused ORF of GAL4–DBD and Met was amplified by PCR and subcloned into NotI and XbaI sites of pAc5.1 ⁄ V5-...

Ngày tải lên: 16/03/2014, 18:20

... our under- standing of the properties and ligand binding of other members of the lipocalin superfamily. This is of partic- ular interest in the light of the development of engineered lipocalins ... and H and part of strand A have b-strand populations greater than 0.9. A persistent section comprising the central regions of strands B, C and D together with pa...

Ngày tải lên: 16/03/2014, 23:20

... difference spectra of the site- directed mutations in the Q o bind- ing site of the bc 1 complex from P. denitrificans,a small bacterial version of the mitochondrial enzyme, allow specific monitoring of the protonation ... for the loss of signal intensity, e.g. for the E81Q mutation, the dependence of the m(C = O) signal for up to 50% of its intensity on...

Ngày tải lên: 23/03/2014, 10:20