Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx

... study by showing the direct interaction of Utp25p with SSU pro-cessome subunits, and the analysis of the 35S pre-rRNAcleavage reactions that are affected by the depletion of Utp25p. Furthermore, ... reactions of the pre-rRNA,among them the endonuclease MRP (responsiblefor the cleavage at site A3in ITS1 ) [3], the box C ⁄ DsnoRNPs U3 and U14, and the box H ⁄ ACA snoRNPssnR10 and snR30, ... UTP-B and UTP-C[18,19]. t-UTP complex binds very early during tran-scription of the pre-rRNA, followed by the UTP-Bcomplex, U3 snoRNP and the Mpp10p complex, and later by Rrp5p and the UTP-C...

Báo cáo khoa học:Chính sách đối ngoại của Đảng với khu vực Đông Nam Á những năm đầu thế kỷ 21 pot

... DONG NAM A NHfiNG NAM DAU THE KY XXI ThS NGUYEN THIHOAN Phien hgp todn the Diin ddn khu vuc ASEAN ldn thUlV, tqi Hd Ngi, ngdy 23-7-2010 Budc sang the ky XXI, nhan thflc dung ... xfl ly cac tinh hudng cu the& quot;'°. Tren thyc te, nudc ta da khai thdc dugc nhieu ngudn lyc, tao dugc the manh cho chien lugc bdo ve To quoc. Cd the coi chien lugc bdo ve ... ra 9 nhiem vu cu the vd 6 bien phdp to chfle thyc hien qud trinh hgi nhdp kinh te quoc te nhdm phdt huy cao do ngi lyc, khai khdc toi da cdc rigudn lyc ben ngodi de tao the vd lyc mdi cho...

Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

... Tropel and J. R. van der Meer1766 FEBS Journal 272 (2005) 1756–1766 ª 2005 FEBS Characterization of HbpR binding by site- directed mutagenesis of its DNA -binding site and by deletion of the effector ... contacts the DNA. The objectives of this work were to identify criticalmotifs in the binding site for HbpR and to examine the necessity of the sensing A -domain of HbpR forDNA binding. The role of ... Here, the HbpR DNA binding interactions were investigated by site- directed mutagenesis of the operator region and by DNA -binding assays using puriﬁed HbpR. Mutations that disrupted the twofold...

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... for the CD11a and CD11b I domains existbecause of the residual binding of the ICAM-4 negativecells. The mAb 60.1 reacting with the CD11b I domain almost completely inhibited the binding of both ... from the ligand binding site on the opposite side of the metal ion-dependentadhesion site (MIDAS) motif, whereas the binding interfacefor mAb 107 seems to overlap that of physiologic ligands and ... The most efﬁcient inhibition of the binding of ICAM-1 L cell transfectants to the I domains wasobtained with the ICAM-1 mAb LB-2 reacting with the ﬁrst domain of ICAM-1 and the mAbs GP8914 and GP8923...

Báo cáo khoa học: Characterization of heme-binding properties of Paracoccus denitriﬁcans Surf1 proteins doc

... concentration of the samples, the protein concentration was determined by the Lowry assay and samples were further analysed by SDS ⁄ PAGE and UV ⁄ Vis spectroscopy.Puriﬁcation of cytochrome c oxidase and ... oxidases and share both structural and functional homologies. Sub-unit I, as the core of the enzyme, houses two heme amoieties (heme a and a3) and a copper ion [1,2]. The biosynthetic pathway of ... CtaA [16]. To further investigatewhether CtaA is the interaction partner for Surf1 and thereby directly supplies the cofactor, we characterized the CtaA protein on a puriﬁed level. The P. denitriﬁcans...

Báo cáo khoa học: Characterization of a high-afﬁnity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt

... Underthese conditions, the speciﬁc radioactivity of the 125I-labelledPA1b, calculated by the ratio of the radioactivity measured by gamma counting and the amount of peptide evaluated by absorbance ... microsomal fraction of anextract of the resistant insect D. melanogaster also displayeda strong binding capacity. The determination of the characteristics of the binding site of these different ... activity [5].Then, the puriﬁcation of the binding site, and the cloning of the corresponding gene in susceptible and resistant strainswill probably help to answer these questions, and willprovide...

Báo cáo khoa học: Characterization of a membrane-bound aminopeptidase puriﬁed from Acyrthosiphon pisum midgut cells A major binding site for toxic mannose lectins pptx

... (2002) Aminopeptidase Nisoforms from the midgut of Bombyx mori and Plutellaxylostella: their classiﬁcation and the factors that deter-mine their binding speciﬁcity of Bacillus thuringiensisCry1Ac ... con-stant against log of EDTA concentration. n, the slope of the plot,was calculated as 0.44 and estimates the number of molecules of EDTA needed to inactivate each active site of the enzyme.CBAFig. ... sequence. The coding protein has high similarity to other ami-nopeptidases. It possesses the domain HEXXH + G,characteristic of gluzincins, and the domain GAMEN,found in many of these enzymes...

Báo cáo khoa học: Characterization of the Drosophila Methoprene -tolerant gene product Juvenile hormone binding and ligand-dependent gene regulation potx

... that the GAL4–DBD fusion of METsits on the UAS of the reporter construct even in the absence of ligand, and that the MET moiety is respon-sible for the observed repression. In the case of verteb-rate ... (Promega); the cDNA fragment containing the fused ORF of GAL4–DBD and Met was ampliﬁed by PCR and subcloned into NotI and XbaI sites of pAc5.1 ⁄V5-His A vector (Invitrogen). The location of the junctionwas ... in the GAL4-MET fusion ⁄ UAS system. The effects of these potential ligands on the reporter activity areshown as fold induction by dividing the activityobtained with the pAcGAL4–DBD–Met by...

Báo cáo khoa học: Characterization of the molten globule state of retinol-binding protein using a molecular dynamics simulation approach ppt

... our under-standing of the properties and ligand binding of othermembers of the lipocalin superfamily. This is of partic-ular interest in the light of the development of engineered lipocalins ... and H and part of strand A have b-strand populations greaterthan 0.9. A persistent section comprising the centralregions of strands B, C and D together with part of strand A in b-sheet 1, and ... mechanism for the binding and release of lig-ands. In the b-lactoglobulins the changes are localizedin the E–F loop, and are prompted by the protonation of a glutamate side chain within the loop....

Báo cáo khoa học: Characterization of mutations in crucial residues around the Qo binding site of the cytochrome bc1 complex from Paracoccus denitriﬁcans pdf

... differencespectra of the site- directed mutations in the Qobind-ing site of the bc1complex from P. denitriﬁcans,asmall bacterial version of the mitochondrial enzyme,allow speciﬁc monitoring of the protonation ... for the loss of signal intensity, e.g. for the E81Q mutation, the dependence of the m(C = O) signalfor up to 50% of its intensity on the orientation of the methoxy side chains in relation to the ... excess of stigmatellin.Upon binding of stigmatellin, shifts reﬂecting the changes within the binding site and the immediate envi-ronment are expected, together with signals for the inhibitor itself,...

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