... the Kdfor the initial bind-ing of minifiber. The fluorescence emission of the TMRwas assumed to be independent of the binding of the minifiber to the penton base, because it is attached at the ... (minifiber), and [Ligtot] is the total con-centration of the ligand. At any total concentration of lig- and, the anisotropy depends on the total concentration of C. Zubieta et al. Human adenovirus ... is the measured anisotropy value, AFis the aniso-tropy of the free minifiber, ABis the anisotropy of the mini-fiber bound to the penton base, [LigB] is the concentration of the bound ligand...