Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt

... mutants of cAMP receptor protein from Escherichia coli. J Biol Chem 278, 43020–43026. 19 Błaszczyk U, Polit A, Guz A & Wasylewski Z (2002) Interaction of cAMP receptor protein from Escherichia coli ... 1001–1007. CRP conformational changes induced by DNA and cAMP M. Tworzydło et al. 1116 FEBS Journal 272 (2005) 1103–1116 ª 2005 FEBS Fluorescence...

Ngày tải lên: 07/03/2014, 16:20

... similar characteristics. Effect of chelators on uptake of NTBI The effects of the chelators on NTBI uptake by hepatocytes and Q7 hepatoma cells were marked and similar. All chelators decreased uptake to 20% or less of the ... contrast to NTBI uptake by Q7 cells, the uptake of Tf-bound Fe (TBI) by receptor- mediated endocytosis is much lower in hepatocytes in compariso...

Ngày tải lên: 20/02/2014, 11:20

... regulatory effects of miR-23a on the expression of IL6R by RT-PCR and western blot, and found an inverse correlation between miR-23a and IL6R expression at both the mRNA and protein levels. To confirm ... miR-23a causes the downregulation of IL6R by binding to its first conserved binding site, which then leads to the development of gastric adeno- carcinoma. Mater...

Ngày tải lên: 18/02/2014, 04:20

... VEGF 121 -induced proliferation downstream from the receptor. Presumably this inhibition occurs at/or upstream from Raf1 and downstream from PLCc. We found the chemokine receptor CXCR3B, a putative PF-4 receptor [34], in ... arget of the tyrosine kinase activity of the KDR and to be involved in VEGF -induced DNA synthesis [31]. P LCc phosphorylation was induced by...

Ngày tải lên: 19/02/2014, 16:20

... and DNA binding to CRP (Eur. J. Biochem. 270) 1423 Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein ... Ligand -induced conformational and structural dynamics changes in Escherichia coli cyclic AMP receptor protein. Bio- chemistry 41, 6660–6667. Ó FE...

Ngày tải lên: 20/02/2014, 23:20

... subdivided into proteins which contribute to the handling of heme and ligation of heme to the apocytochrome, and those involved in the provision of reductant to the apocytochrome in order to reduce ... addi- tion of reductant is a result of the reduction of a disul- fide in the apocytochrome. Production of endogenous E. coli cytochromes Escherichia coli contains...

Ngày tải lên: 06/03/2014, 09:22

... interpretation of the origin of the static quenching. However, judging from the binding stoichi- ometry together with the shape of the binding curves (Fig. 3), it is safe to conclude that the quenching ... 5405 inititator, upon binding to origin DNA. J Biol Chem 278, 18606–18616. 4 Giraldo R, Andreu JM & Dı ´ az-Orejas R (1998) Protein domains and conformational...

Ngày tải lên: 07/03/2014, 04:20

... growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor Tomio Hashimoto 1 , Minoru Kato 2 , Takeshi Shimomura 2 and Naomi Kitamura 1 1 Department of Biological Sciences, ... hepatocyte growth factor activator inhibitor type 2; HGF, hepatocyte growth factor; HGFA, hepatocyte growth factor activator; HPAI, highly pathogenic avian influenza; IC 50, the concentrat...

Ngày tải lên: 15/02/2014, 01:20

... presence of ADP and Mg 2+ and followed by nondenaturing PAGE, the bands of the enzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivase complexes (upper and ... Positions of DD and its subun- its (a D , b D and c D ) and the reactivase (DD-R) and its subunits (a R and b R ) are indicated on the right of the gels. In (C)...

Ngày tải lên: 15/02/2014, 01:20

... transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of subunit 8 and helix a of cytochrome b. The figure was constructed from the crystal structure of the yeast ... inner membrane of mitochondria [1,2]. This respiratory enzyme catalyzes the transfer of electrons from ubiquinol to cytochrome c and couples the electron tr...

Ngày tải lên: 19/02/2014, 12:20