Báo cáo khoa học: Modulation of IMPDH2, survivin, topoisomerase I and vimentin increases sensitivity to methotrexate in HT29 human colon cancer cells docx

... type II is significantly up-regulated in malig- nant cells [21,22]. Benzamide riboside, tiazofurine and mycophenolic acid are potent inhibitors of this enzy- mic activity and phase II ⁄ III clinical ... in the absence and in the presence of MTX. Targeting of vimentin was carried out by lipofecting aODN-VIM into paren- tal HT29 cells causing a decrease of 60% in th...

Ngày tải lên: 07/03/2014, 16:20

... FEBS and ⁄ or its interaction with other proteins such as the PSI core. Incorporation of tagged PSI-G into PSI Whilst it is evident that both C-terminally and loop- tagged PSI-G can insert into ... PSI-G into Arabidopsis PSI complexes in vivo .In achieving this, we have been able to collect multiple lines of evidence that PSI-G inserts into the thylakoid mem- brane with a stromal...

Ngày tải lên: 07/03/2014, 21:20

... Machida Y, Miyazaki H, Mitsui K, Kuroiwa Y et al. (2008) RNA-binding protein TLS is a major nuclear aggregate-interacting protein in hunting- tin exon 1 with expanded polyglutamine-expressing cells. ... metabolite of MPTP. This leads to an increase in the concentration of MPP + in the dopa- minergic neurons, leading to selective damage to sub- stantia nigra, similar to idio...

Ngày tải lên: 14/02/2014, 19:20

... intact mitochondria, it is anticipated that there may be a significant production of P i in the vicinity of the ATPase within the matrix. CYPD ablation or its inhibition by cyclosporin A increases ... P i con- centration, which in turn could affect CYPD binding to F 0 F 1 -ATP synthase, and decreased binding of the inhibitory protein IF 1 to ATPase. IF1 is a naturally occ...

Ngày tải lên: 14/02/2014, 19:20

... plasmid combinations were spotted either onto medium lacking histidine and leucine to select for the plasmids, or onto medium lacking histi- dine, leucine and uracil to monitor protein interactions. ... used in this work are listed in Table 5. Split-ubiquitin technique For the split-ubiquitin interaction assays, 10 5 wild-type cells carrying the split-ubiquitin plasmids were spo...

Ngày tải lên: 18/02/2014, 13:20

... tyrosine residues interact with each side of the isoalloxazine in plastidic FNRs. On the si-face of the flavin, which is buried within the protein structure, a tyrosine aromatic side-chain (Y89 in ... may constrain the nicotinamide and or the terminal tyrosine against the flavin. It has been described that the bind- ing of NADH to lactate dehydrogenase conditions the nicotinamide...

Ngày tải lên: 18/02/2014, 17:20

... quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. 26 Castellani O, Ribeiro LV & Fernandes JF (1967) Differ- entiation of Trypanosoma cruzi in culture. ... all our attempts to detect ODC or ADC enzy- matic activities in various strains of wild-type T. cruzi epimastigotes, by adding radioactive ornithine or arginine to intact par...

Ngày tải lên: 19/02/2014, 07:20

... critical r ole in the regulation of cell proliferation, including SMC, by inhibiting signalling mol- ecules [ 45–47]. Caveolin-3 is the main isoform in muscle cells, including SMC [30]. Caveolin-1 ... in AA-induced proliferation using cells incubated w ith t he MAPK kinase (MEK) inhibitor, U0126. U0126 completely inhibited the cyclin D1 promoter activity in AA-pretreated SMC incu...

Ngày tải lên: 19/02/2014, 16:20

... CBP (cAMP-response-element-binding protein-binding protein) interacting sites in GRIP1 (glucocorticoid receptor-interacting protein), and their importance for the function of GRIP1. Biochem J 382, 111–119. 27 ... 2183 Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-associa...

Ngày tải lên: 07/03/2014, 12:20

... preference of zinc in the more flexible b-domain. In addition to the already described differences in flexibility of the b-domain in MT isoforms 1 and 2 [8], we found that the domain specific distinctions ... that in vitro it suppresses the metal release from metallothioneins. This points to an hitherto unknown mechanism or compound(s) being involved in this inter- action...

Ngày tải lên: 07/03/2014, 15:20