Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export pptx

... Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat- mediated export Wouter S. P. Jong 1 , Corinne M. ten Hagen-Jongman 1 , ... pTorA/P2 [ 17] a s a template and the primers RRTorA-SacI-fw (5¢-GCGCG GAGCTCAAGAAGGA AGAAAAATAATGAAC-3¢, SacI site underlined) and TorA/Lep2-BamHI-rv (5¢-G...

Ngày tải lên: 07/03/2014, 16:20

... USA Accurate aminoacylation of tRNA by aminoacyl-tRNA synthetases (aaRSs) is a crucial step in the faithful translation of mRNA. In addition to their fundamental role in translation, aaRSs are ... A freestanding proofreading domain is required for pro- tein synthesis quality control in Archaea. Proc Natl Acad Sci USA 101, 10260–10265. 29 Dagkessamanskaia A, Martin-Yken H,...

Ngày tải lên: 23/03/2014, 09:20

... affects Raf1 activity in HUVEC. Increases in cAMP levels and the activation of the cAMP-dependent protein kinase A (PKA) may be involved [44]. Indeed, P KA inhibits the MAP k inase pathway by blocking ... (PLCc), a s ubstrate of KDR kinase, i s then phosphorylated and activated, leading to the activation of p rotein kinase C (PKC), followed by the serine/ threonine kin...

Ngày tải lên: 19/02/2014, 16:20

... findings may explain why bisecting GlcNAc-containing N-glycans are abundant in the brain [65]. In fact, mice carrying an inactive GnT-III mutant have an atypical neurolog- ical phenotype [66]. The ... has a large extracellular region, a single transmembrane domain, and a short cytoplasmic tail (except for b 4 ). The N-terminal domains of the a- and b-subunits associate to...

Ngày tải lên: 18/02/2014, 17:20

... the coiled-coil region of c and the complete a- helical C-terminus as held within the top of (ab) 3 . The rotary axis z was aligned along the main axis of the shaft. The ‘bearing’ included a total of 138 residues ... revealed that rotation around the w Ramachandran angle w as preferred over that around /. We calculated the potential barrier f or the rotation aro...

Ngày tải lên: 19/02/2014, 16:20

... to the sum of a number of two-state transitions. In these fittings the heat capacity change, DC p ,forthe unfolding of each domain was fixed by using the values obtained from the analysis of the ... from these equations using the following set of parameters: a linear heat capacity function for the native state, C p (N); the enthalpies and the heat capacities o...

Ngày tải lên: 21/02/2014, 03:20

... anticancer activity in tumour xenograft models, which indicate that the approach may be applicable to the treatment of a wide range of human cancers. This minireview summarizes the available data on these compounds ... p16 INK 4a kinase and p53 pathways [32–35] which can be visualized by the appearance of charac- teristic DNA damage foci using an antibody to the damage re...

Ngày tải lên: 06/03/2014, 09:22

... profiling of early lateral root initiation. Proc Natl Acad Sci USA 101, 5146–5151. 22 Inukai Y, Sakamoto T, Ueguchi-Tanaka M, Shibata Y, Gomi K, Umemura I, Hasegawa Y, Ashikari M, Kit- ano H & ... construction and sequencing For Solexa tag preparation and sequencing, 1 lg of total RNA was incubated with oligo(dT) beads to capture the mRNA with a poly (A) tail. First- and second...

Ngày tải lên: 22/03/2014, 17:20

... the 16-h incubations, in which at least 50% of residual lig- and-binding capacity was maintained at the highest [HNE] ⁄ [OBP] values. In the case of 20-min incuba- tions, HNE inactivation was even ... HNE and AMA are shown for porcine (C) and bovine (D) OBP. Protein samples were incubated with a fixed saturating amount of AMA and increasing HNE. Each point on the y-axis s...

Ngày tải lên: 23/03/2014, 10:20

... bottle of wine on the table or the 250ml in this glass. Note that these can again be abstract entities: but rather than potentially being abstract conventional kinds of wine, they can be abstract ... as Wine is wine (two readings, both analytic) Wine is a wine (false) All wine is wine (analytic) Claret is a wine (true) Cheap wine is a wine (false) *All wine is a w...

Ngày tải lên: 24/03/2014, 01:21