Báo cáo khoa học: New human and mouse microRNA genes found by homology search pptx

... Clusters of human microRNAs. M. J. Weber New human and mouse microRNA gene FEBS Journal 272 (2005) 59–73 ª 2004 FEBS 73 New human and mouse microRNA genes found by homology search Michel J. Weber Laboratoire ... genes, and to the predic- tion of new mRNA targets. Results and Discussion New potential human and mouse microRNA precursors The entir...

Ngày tải lên: 07/03/2014, 16:20

... Science and Technol- ogy Agency, the Suzuken Memorial Foundation, the Sumitomo Foundation, the Gushinkai Foundation (to K. Fujimori), and the Takeda Science Foundation (to K. Fujimori and Y. Fujitani). References 1 ... liver and WAT. Paraffin sections of liver and WAT from WT and TG mice (S55) were stained with antibody against human H-PGDS. Bars: 100 lm. (D) PGDS activity in liv...

Ngày tải lên: 16/02/2014, 09:20

... sardines, herrings and anchovies from tropical seas, causing neurological and gastrointestinal disturbances asso- ciated with clupeotoxism [1]. Symptoms include a bitter and metallic taste followed by nausea, ... these toxins have been found not only in zooanthids but in sea anemo- nes, polychaete worms, crabs, and herbivorous fishes, probably due to an accumulation of the toxin b...

Ngày tải lên: 18/02/2014, 14:20

... mutagenesis rate in bacteria [11] and increases the risk of cancer devel- opment in mouse models [12,13] and in humans [14,15]. Discrimination in favor of 8-oxoG:C and against 8-oxoG:A mispairs by ... deleterious effect on k 2 and k 3 for 8-oxoG:A substrates: 20 mm Mg 2+ decreased k 2 by 42-fold and k 3 by 5.5-fold, whereas 150 mm KCl decreased k 2 by 3.9-fold and k 3 b...

Ngày tải lên: 18/02/2014, 18:20

... dNTPs, 1 mm MgCl 2 and 0.025 unitsÆlL )1 Taq polymerase (Finnzymes, Espoo, Finland). The amplified PCR products were separated on a 2% agarose gel, bands were excised and purified by using the High ... calibration curves and data were normalized to the copy number of 18S RNA. Expression of ACMSD isoforms in E. coli pGEM-ACMSD I and pGEM-ACMSD II were digested with XhoI and Bpu1102I...

Ngày tải lên: 19/02/2014, 02:20

... receptor and bHLH domains suggest that they were expanded by a diversification process derived from common ancestral genes via duplication and exon shuffling [28,55]. They are believed to have expanded ... 2658–2663. 67 Newman M (2002) Random graphs as models of net- works. Handbook of Graphs and Networks (Bornholdt, S & Schuster, HG, eds). Wiley-VHC, Weinheim. 68 Vousden KH &...

Ngày tải lên: 19/02/2014, 07:20

... RT-PCR with primer pairs specific for human Ras-GRF1. Primer pairs: lane 2, ON359 and ON360; lane 3, ON357 and ON358; and lane 4, ON361 and ON360. The PCR prod- ucts and a DNA size marker, lane 1, were ... was supported by The Norwegian Council on Cardiovascular Diseases, The Norwegian Cancer Soci- ety, The Research Council of Norway, The Novo Nor- disk Foundation, Anders Jahre’s...

Ngày tải lên: 19/02/2014, 18:20

... nearly identical manner by m onomeric and dimeric b1IV and the proteoglycan form and by laminin-1 (a1b1c1) derived from the mouse EHS tumor (Fig. 5A). Laminin-1 fragment E10 showed a less steep and incomplete ... by the b1IV and b2IV assay and also by an assay speci®c for the laminin c1 chain [37]. This demonstrated that 71±93% of the extractable laminin c1, b1 and b2...

Ngày tải lên: 21/02/2014, 03:20

... cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 were separated by SDS-PAGE, blotted and probed with the a-His antibody and the a-Scpep1 antisera from rabbit and rat, respectively. ... supported by the Deutsche Forschungsgemeinschaft (Grants LU 1173 ⁄ 1-1 and 1-2). The mouse ES cell line RST426 was provided by Baygenomics, a Program for Genetic Applicat...

Ngày tải lên: 07/03/2014, 03:20

... C-type lectin proteins and metalloproteases [36,37], and used to amplify specific cDNA by PCR. For cloning of LC 1and LC2, the sense primer was 5¢-GGAA(C/G)GAAG(A/G)CCATGGGGCG-3¢ and the antisense primer ... epitopes Samples of 7 lg of RVV-X and 5 lg of BSA were analysed by 8% SDS-PAGE under reducing conditions. Appropriate amounts of Le x/a - and SLe x/a -conjugated BSAs and...

Ngày tải lên: 07/03/2014, 06:20