Báo cáo khoa học: New human and mouse microRNA genes found b

Báo cáo khoa học: New human and mouse microRNA genes found by homology search pptx

... Clusters of human microRNAs. M. J. Weber New human and mouse microRNA gene FEBS Journal 272 (2005) 59–73 ª 2004 FEBS 73 New human and mouse microRNA genes found by homology search Michel J. Weber Laboratoire ... genes, and to the predic- tion of new mRNA targets. Results and Discussion New potential human and mouse microRNA precursors The entir...

Ngày tải lên : 07/03/2014, 16:20

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Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx

... Science and Technol- ogy Agency, the Suzuken Memorial Foundation, the Sumitomo Foundation, the Gushinkai Foundation (to K. Fujimori), and the Takeda Science Foundation (to K. Fujimori and Y. Fujitani). References 1 ... liver and WAT. Parafﬁn sections of liver and WAT from WT and TG mice (S55) were stained with antibody against human H-PGDS. Bars: 100 lm. (D) PGDS activity in liv...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: a new view of palytoxin toxicity ppt

... sardines, herrings and anchovies from tropical seas, causing neurological and gastrointestinal disturbances asso- ciated with clupeotoxism [1]. Symptoms include a bitter and metallic taste followed by nausea, ... these toxins have been found not only in zooanthids but in sea anemo- nes, polychaete worms, crabs, and herbivorous ﬁshes, probably due to an accumulation of the toxin b...

Ngày tải lên : 18/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the speciﬁcity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf

... mutagenesis rate in bacteria [11] and increases the risk of cancer devel- opment in mouse models [12,13] and in humans [14,15]. Discrimination in favor of 8-oxoG:C and against 8-oxoG:A mispairs by ... deleterious effect on k 2 and k 3 for 8-oxoG:A substrates: 20 mm Mg 2+ decreased k 2 by 42-fold and k 3 by 5.5-fold, whereas 150 mm KCl decreased k 2 by 3.9-fold and k 3 b...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... dNTPs, 1 mm MgCl 2 and 0.025 unitsÆlL )1 Taq polymerase (Finnzymes, Espoo, Finland). The ampliﬁed PCR products were separated on a 2% agarose gel, bands were excised and puriﬁed by using the High ... calibration curves and data were normalized to the copy number of 18S RNA. Expression of ACMSD isoforms in E. coli pGEM-ACMSD I and pGEM-ACMSD II were digested with XhoI and Bpu1102I...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Topology, tinkering and evolution of the human transcription factor network doc

... receptor and bHLH domains suggest that they were expanded by a diversiﬁcation process derived from common ancestral genes via duplication and exon shufﬂing [28,55]. They are believed to have expanded ... 2658–2663. 67 Newman M (2002) Random graphs as models of networks. Handbook of Graphs and Networks (Bornholdt, S & Schuster, HG, eds). Wiley-VHC, Weinheim. 68 Vousden KH &...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... RT-PCR with primer pairs speciﬁc for human Ras-GRF1. Primer pairs: lane 2, ON359 and ON360; lane 3, ON357 and ON358; and lane 4, ON361 and ON360. The PCR products and a DNA size marker, lane 1, were ... was supported by The Norwegian Council on Cardiovascular Diseases, The Norwegian Cancer Soci- ety, The Research Council of Norway, The Novo Nor- disk Foundation, Anders Jahre’s...

Ngày tải lên : 19/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents pptx

... nearly identical manner by m onomeric and dimeric b1IV and the proteoglycan form and by laminin-1 (a1b1c1) derived from the mouse EHS tumor (Fig. 5A). Laminin-1 fragment E10 showed a less steep and incomplete ... by the b1IV and b2IV assay and also by an assay speci®c for the laminin c1 chain [37]. This demonstrated that 71±93% of the extractable laminin c1, b1 and b2...

Ngày tải lên : 21/02/2014, 03:20

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Báo cáo khoa học: Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1 ppt

... cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 were separated by SDS-PAGE, blotted and probed with the a-His antibody and the a-Scpep1 antisera from rabbit and rat, respectively. ... supported by the Deutsche Forschungsgemeinschaft (Grants LU 1173 ⁄ 1-1 and 1-2). The mouse ES cell line RST426 was provided by Baygenomics, a Program for Genetic Applicat...

Ngày tải lên : 07/03/2014, 03:20

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Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot

... C-type lectin proteins and metalloproteases [36,37], and used to amplify speciﬁc cDNA by PCR. For cloning of LC 1and LC2, the sense primer was 5¢-GGAA(C/G)GAAG(A/G)CCATGGGGCG-3¢ and the antisense primer ... epitopes Samples of 7 lg of RVV-X and 5 lg of BSA were analysed by 8% SDS-PAGE under reducing conditions. Appropriate amounts of Le x/a - and SLe x/a -conjugated BSAs and...

Ngày tải lên : 07/03/2014, 06:20

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