Báo cáo khoa học: The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitiv pptx
Báo cáo khoa học: The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitiv pptx
... 2004 The MCAD Y42H mutation is temperature sensitive (Eur. J. Biochem. 271) 4063 The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, ... observed,indicating that the temperature- induced change in the foldof the structure is indistinguishable between the threeproteins at this protein concentration. Furthermore, this temperature- induced ... thusconfirming that the folding change is irreversible.One could thus envisage that by increasing the stressplaced on the MCAD tetramer, i.e. by increasing the temperature, the t etramer has an increased...
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Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf
... the peakof the emission spectrum at 342 nm. The concentrationof Na+was increased by aspirating a defined volume ofthrombin solution dissolved in the cuvette in the above buf-fer containing ... of the reduced A-chain in the mutant enzyme. The sodium ion bind-ing affinity was not significantly perturbed by Lys9 deletion, although the linked increase in intrinsic fluorescence was lower in ... side-chainsto the solvent the smaller the intrinsic fluorescence of the enzyme. The smaller gain in the intrinsic fluorescence of DK9upon Na+concentration (9 vs. 18% in WT) may reflectnot only the...
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Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc
... PKCf in signaltransduction. Our results indicate that PKCf is not involved in the activation of glycogen synthesis by insulin. Thisfinding is in good agreement with a previous report showingthat ... taken and the experimentwas started by the addition of agonists to the dishes.Inhibitors were added 10 min before the agonists. The incubation was terminated by rapidly aspirating the medium ... synthesis by insulin could not beinhibited by the relatively selective PKC inhibitor bis-indolylmaleimide I, which predominantly inhibits conven-tional and novel isoforms, i.e. the a-, d-ande-isoforms(Fig....
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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
... are classified intothree groups, the neutral subtilisins, the alkaline subtilisinsand the ALP I-typeÕ subtilisin (Fig. 1) [3]. The neutralsubtilisins consist of the subtilisins from neutrophilicBacillus ... on the molecular surface. In the wild-type enzyme, the side chain of Asp266 interacted with the amino group of the main chain at Glu268. The oxygen of the main chain at Asp266 was bound to amino ... surrounding residues of the depression, and the side chain of the mutated residue might restrain the mobility of the N-terminal region on the surface by interaction with the main chain of Lys26 by...
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Báo cáo khoa học: The fate of newly synthesized V-ATPase accessory subunit Ac45 in the secretory pathway doc
... V-ATPase is requiredfurther downstream in the secretory pathway but it is notknown in which compartment the V-ATPase becomesactive and which mechanism is involved in the targeting of the V-ATPase ... completed in t his c ompartment by the build-up of the V1onto the V0[28,29]. Given the pH in the ER, the V-ATPaseshould be considered as being essentially inactive in this partof the secretory ... bovine serumalbumin and 2 mg glucose] for 30 min at 22 °C. P ulselabeling of newly synthesized proteins was performed by incubating the lobes in methionine/cysteine-free culturemedium containing...
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Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx
... conserved kinasedomain [7]. In tomato, PTI1 can physically interactwith the serine ⁄ threonine kinase PTO, which confersresistance to the bacterial pathogen P. syringae pvtomato carrying the avirulence ... & Martin GB (1995) The tomato gene Pti1 encodes a serine ⁄ threonine kinase that is phosphorylated by Pto and is involved in the hyper-sensitive response. Cell 83, 925–935.18 Martin GB, Brommonschenkel ... PINOID. Cell 100, 469–478.12 Benjamins R, Quint A, Weijers D, Hooykaas P &Offringa R (2001) The PINOID protein kinase regulatesorgan development in Arabidopsis by enhancing polarauxin...
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Tài liệu Báo cáo khoa học: The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress docx
... harbours an iscR::lacZ fusion [42].IscR is a Fe ⁄ S protein encoded by the first gene of the isc operon, and it is involved in the transcriptionalregulation of both the isc and the suf operons. In itsholoform, ... bodies.Because the histidine-tag can in uence the solubilityand the folding of the protein in some cases, we alsopurified the OsiS protein without any tag, using amono-Q purification column. This method ... associatedwith the protein moiety. The addition of 0.1 mm cyste-ine to the protein sample induced a shift in the majorpeak in the range 390–420 nm, which indicates thatsome interaction occurred with the...
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Báo cáo khoa học: An inserted loop region of stromal ascorbate peroxidase is involved in its hydrogen peroxide-mediated inactivation pot
... microbody-localizedAPXs of higher plants [8]. This finding raised the hypothesis that a region within the C-terminal half of the chloroplastic APXs is involved in their susceptibil-ity to hydrogen peroxide.ResultsPreparation ... isoforms in chloroplasts, one of which is soluble in the stroma and the other of which is bound to the stromal side of thylakoid membranes.Both isoforms are involved in the water–water cycle, asystem ... purifiedfrom spinach [5]. These results indicated that an inser-ted loop region of stromal APX is involved in itshydrogen peroxide-mediated inactivation.Discussion In this study, by using various...
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Báo cáo khoa học: Glycogen synthase kinase 3b and b-catenin pathway is involved in toll-like receptor 4-mediated NADPH oxidase 1 expression in macrophages ppt
... to the pathogenesis of atherosclerosis. In the vascular system, the major source of reactive oxygen species is the NADPH oxidase (Nox) family. Nox1 is induced by lipopolysaccharide(LPS) in macrophages, ... supported by fluores-cent microscopy. In untreated cells, b-catenin wasmainly localized in the cytosol at a low level. After45 min of treatment with LPS, the distribution ofb-catenin in the subcellular ... (ng·mL–1)ββ-cateninβ-actinA0 15 30 45 60 LPS (min)β-cateninβ-actinFig. 3. Changes in the subcellular localization of b-catenin induced by LPS in macrophages. (A,B) Raw264.7 cells were incubated...
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Báo cáo khoa học: The highly conserved extracellular peptide, DSYG(893–896), is a critical structure for sodium pump function docx
... concentrations of KCl. The other conditions wereas described above. In all cases, K+leads t o a reduction of ouabainbinding. (B) By plottin g the reciprocal binding of [3H]ouabain against the K+concentration, ... explained on t he basis of structuraldisturbances leading to the loss of a/b subunit interactions.Together with the results of previous investigations, show-ing the involvement of amino acids ... for interactions with the bsubunit, the L7/8 region of the a subunit also seems tobe involved in ion translocation or rec ognition. This is supported by the results of various investigations involvingeither...
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