Báo cáo khoa học: The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitiv pptx
Báo cáo khoa học: The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitiv pptx
... 2004 The MCAD Y42H mutation is temperature sensitive (Eur. J. Biochem. 271) 4063 The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, ... observed, indicating that the temperature- induced change in the fold of the structure is indistinguishable between the th...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf
... the peak of the emission spectrum at 342 nm. The concentration of Na + was increased by aspirating a defined volume of thrombin solution dissolved in the cuvette in the above buf- fer containing ... of the reduced A-chain in the mutant enzyme. The sodium ion bind- ing affinity was not significantly perturbed by Lys9 deletion, although the linked increase in intrinsi...
Ngày tải lên: 07/03/2014, 12:20
Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc
... PKCf in signal transduction. Our results indicate that PKCf is not involved in the activation of glycogen synthesis by insulin. This finding is in good agreement with a previous report showing that ... taken and the experiment was started by the addition of agonists to the dishes. Inhibitors were added 10 min before the agonists. The incubation was terminated by rap...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
... are classified into three groups, the neutral subtilisins, the alkaline subtilisins and the ALP I-typeÕ subtilisin (Fig. 1) [3]. The neutral subtilisins consist of the subtilisins from neutrophilic Bacillus ... on the molecular surface. In the wild- type enzyme, the side chain of Asp266 interacted with the amino group of the main chain at Glu268. The oxygen of the main...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: The fate of newly synthesized V-ATPase accessory subunit Ac45 in the secretory pathway doc
... V-ATPase is required further downstream in the secretory pathway but it is not known in which compartment the V-ATPase becomes active and which mechanism is involved in the targeting of the V-ATPase ... completed in t his c ompartment by the build-up of the V 1 onto the V 0 [28,29]. Given the pH in the ER, the V-ATPase should be considered as being es...
Ngày tải lên: 31/03/2014, 09:20
Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx
... conserved kinase domain [7]. In tomato, PTI1 can physically interact with the serine ⁄ threonine kinase PTO, which confers resistance to the bacterial pathogen P. syringae pv tomato carrying the avirulence ... & Martin GB (1995) The tomato gene Pti1 encodes a serine ⁄ threonine kinase that is phosphorylated by Pto and is involved in the hyper- sensitive response. Cel...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress docx
... harbours an iscR::lacZ fusion [42]. IscR is a Fe ⁄ S protein encoded by the first gene of the isc operon, and it is involved in the transcriptional regulation of both the isc and the suf operons. In its holoform, ... bodies. Because the histidine-tag can in uence the solubility and the folding of the protein in some cases, we also purified the OsiS protein witho...
Ngày tải lên: 18/02/2014, 04:20
Báo cáo khoa học: An inserted loop region of stromal ascorbate peroxidase is involved in its hydrogen peroxide-mediated inactivation pot
... microbody-localized APXs of higher plants [8]. This finding raised the hypothesis that a region within the C-terminal half of the chloroplastic APXs is involved in their susceptibil- ity to hydrogen peroxide. Results Preparation ... isoforms in chloroplasts, one of which is soluble in the stroma and the other of which is bound to the stromal side of thylakoid membra...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Glycogen synthase kinase 3b and b-catenin pathway is involved in toll-like receptor 4-mediated NADPH oxidase 1 expression in macrophages ppt
... to the pathogenesis of atherosclerosis. In the vascular system, the major source of reactive oxygen species is the NADPH oxidase (Nox) family. Nox1 is induced by lipopolysaccharide (LPS) in macrophages, ... supported by fluores- cent microscopy. In untreated cells, b-catenin was mainly localized in the cytosol at a low level. After 45 min of treatment with LPS, the...
Ngày tải lên: 22/03/2014, 21:21
Báo cáo khoa học: The highly conserved extracellular peptide, DSYG(893–896), is a critical structure for sodium pump function docx
... concentrations of KCl. The other conditions were as described above. In all cases, K + leads t o a reduction of ouabain binding. (B) By plottin g the reciprocal binding of [ 3 H]ouabain against the K + concentration, ... explained on t he basis of structural disturbances leading to the loss of a/b subunit interactions. Together with the results of previous investigations, show-...
Ngày tải lên: 23/03/2014, 13:20