Báo cáo khoa học: Testosterone 1b-hydroxylation by human cytochrome P450 3A4 pdf

... Of several human P450s examined, only P450 3A4 formed this product. The p roduct was also formed in human liver microsomes. Keywords: cytochrome P450; NMR spectroscopy; HPLC- NMR combinations; testosterone. Cytochrome ... Testosterone 1b-hydroxylation by human cytochrome P450 3A4 Joel A. Krauser 1 , Markus Voehler 2 , Li-Hong Tseng 3 , Alexandre ... high and low in...

Ngày tải lên: 07/03/2014, 16:20

... followed by Strep-tag II was isolated by BamHI digestion and inserted into the BamHI site of pmGFP–calpain 7. To construct pCMV3xFLAG–IST1 and pGEX–IST1, an IST1 cDNA fragment was amplified by PCR, ... pelleted by brief centrifugation (1 000 g, 1 min) and washed three times with buffer B, bound protein complexes were separated on a 15% gel by SDS ⁄ PAGE. Protein bands were detected...

Ngày tải lên: 18/02/2014, 04:20

... Discussion Identification of the group inducing tetramerization of human TK1 Human TK1 has 234 amino acids and a subunit size of 25.5 kDa [18]. Several reports have shown by gel filtration that native as well as recombinant ... thymidine kinase 1 of human and mycoplasmic origin. Proc Natl Acad Sci USA 101, 17970–17975. 15 Sabini E, Ort S, Monnerjahn C, Konrad M & Lavie A (2003) Stru...

Ngày tải lên: 18/02/2014, 12:20

... mAb did not show any cross-reactivity to other proteins, such as human serum albumin (HSA), BSA, human IgA, human IgM, human IgE, human hemoglobin, KLH and tumor necrosis factor-b (TNF-b)(Fig. ... [13]. Recombinant human hTNF-a was gradually degraded by ETNF-6-H during 94 h of incubation. The cleavage sites, which were confirmed by N-termi- nal amino acid sequencing, are indica...

Ngày tải lên: 06/03/2014, 22:21

... transport by uptake to inside-out vesicles from human erythrocytes. Biochim Biophys Acta 1463, 121–130. 13 Boadu E & Sager G (2000) ATPase activity and trans- port by a cGMP transporter in human ... in human red cell membranes. Biochem Pharmacol 70, 500–510. 19 Boadu E & Sager G (2004) Reconstitution of ATP- dependent cGMP transport into proteoliposomes by membrane protein...

Ngày tải lên: 07/03/2014, 11:20

... the intestinal scrapings by a-amylase present in the hetero- geneous sample or by activity in the C-terminal domain of the full-size protein, thereby accounting for the inhibition by ‘acarbose’ reported ... determined by SDS ⁄ PAGE analysis (Fig. 3, inset) and by mass spec- trophotometric analysis. The expected size of the 876 amino acid expressed domain is 99 274 while a mass of 10...

Ngày tải lên: 07/03/2014, 12:20

... Consequently, transactivation of the four respective genes by FOXM1c should increase proliferation. By contrast, repression of these genes by dominant-negative FOXM1c should reduce proliferation. ... transforming human papilloma- virus 16 (HPV16) binds directly to FOXM1c [28]. Figure 10A shows that the transactivation of the c-myc promoter by FOXM1c and FOXM1c(189–762) was significan...

Ngày tải lên: 19/02/2014, 05:20

... the Tyr232 residues were replaced by alanine (Y232A) and tryptophan (Y232W), respectively, by site directed mutagenesis, and purified them to near homo- geneity by a four-step purification method ... verified by fluorescent spectroscopic analyses of tryptophan residues inserted in this loop. The importance of the L1 loop for DNA binding by RecA ⁄ Rad51 family proteins was observed by...

Ngày tải lên: 19/02/2014, 06:20

... 3-methylglutaconic acid. A protein was purified from human brain cells by affinity chro- matography using the immobilized RNA-oligonucleo- tide (AUUUA) 5 or ‘AU’ followed by cloning of the corresponding gene ... accordingly. Also in this reaction two compounds were produced by glut- aconate CoA-transferase. The molecules were separ- ated by HPLC, analyzed by mass spectrometry and we...

Ngày tải lên: 19/02/2014, 07:20

... rarely operates in isolation. Rather the resultant Ca 2+ signals evoked by this molecule are often boos- ted by Ca 2+ release by RyRs, InsP 3 Rs or both. Inter- actions between different Ca 2+ release ... from [22]. M. Yamasaki et al. Calcium signalling by NAADP FEBS Journal 272 (2005) 4598–4606 ª 2005 FEBS 4599 Ca 2+ release channels modulated by either InsP 3 or cADPR that partici...

Ngày tải lên: 20/02/2014, 01:20