... Of
several human P450s examined, only P450 3A4 formed this
product. The p roduct was also formed in human liver
microsomes.
Keywords: cytochrome P450; NMR spectroscopy; HPLC-
NMR combinations; testosterone.
Cytochrome ... Testosterone 1b-hydroxylation by human cytochrome P450 3A4
Joel A. Krauser
1
, Markus Voehler
2
, Li-Hong Tseng
3
, Alexandre ... high and low in...
... followed by Strep-tag II
was isolated by BamHI digestion and inserted into the
BamHI site of pmGFP–calpain 7.
To construct pCMV3xFLAG–IST1 and pGEX–IST1, an
IST1 cDNA fragment was amplified by PCR, ... pelleted by brief centrifugation
(1 000 g, 1 min) and washed three times with buffer B, bound
protein complexes were separated on a 15% gel by SDS ⁄
PAGE. Protein bands were detected...
... Discussion
Identification of the group inducing
tetramerization of human TK1
Human TK1 has 234 amino acids and a subunit size
of 25.5 kDa [18]. Several reports have shown by gel
filtration that native as well as recombinant ... thymidine kinase 1 of human and
mycoplasmic origin. Proc Natl Acad Sci USA 101,
17970–17975.
15 Sabini E, Ort S, Monnerjahn C, Konrad M & Lavie A
(2003) Stru...
... mAb did
not show any cross-reactivity to other proteins, such
as human serum albumin (HSA), BSA, human IgA,
human IgM, human IgE, human hemoglobin, KLH
and tumor necrosis factor-b (TNF-b)(Fig. ... [13].
Recombinant human hTNF-a was gradually
degraded by ETNF-6-H during 94 h of incubation.
The cleavage sites, which were confirmed by N-termi-
nal amino acid sequencing, are indica...
... transport by
uptake to inside-out vesicles from human erythrocytes.
Biochim Biophys Acta 1463, 121–130.
13 Boadu E & Sager G (2000) ATPase activity and trans-
port by a cGMP transporter in human ... in human red cell membranes. Biochem
Pharmacol 70, 500–510.
19 Boadu E & Sager G (2004) Reconstitution of ATP-
dependent cGMP transport into proteoliposomes by
membrane protein...
... the
intestinal scrapings by a-amylase present in the hetero-
geneous sample or by activity in the C-terminal
domain of the full-size protein, thereby accounting for
the inhibition by ‘acarbose’ reported ... determined by
SDS ⁄ PAGE analysis (Fig. 3, inset) and by mass spec-
trophotometric analysis. The expected size of the 876
amino acid expressed domain is 99 274 while a mass
of 10...
... Consequently,
transactivation of the four respective genes by FOXM1c
should increase proliferation. By contrast, repression of
these genes by dominant-negative FOXM1c should
reduce proliferation. ... transforming human papilloma-
virus 16 (HPV16) binds directly to FOXM1c [28].
Figure 10A shows that the transactivation of the c-myc
promoter by FOXM1c and FOXM1c(189–762) was
significan...
... the Tyr232 residues were replaced by alanine
(Y232A) and tryptophan (Y232W), respectively, by site
directed mutagenesis, and purified them to near homo-
geneity by a four-step purification method ... verified by fluorescent
spectroscopic analyses of tryptophan residues inserted
in this loop. The importance of the L1 loop for DNA
binding by RecA ⁄ Rad51 family proteins was observed
by...
... 3-methylglutaconic acid. A protein
was purified from human brain cells by affinity chro-
matography using the immobilized RNA-oligonucleo-
tide (AUUUA)
5
or ‘AU’ followed by cloning of the
corresponding gene ... accordingly. Also in
this reaction two compounds were produced by glut-
aconate CoA-transferase. The molecules were separ-
ated by HPLC, analyzed by mass spectrometry and
we...
... rarely operates in isolation. Rather the resultant
Ca
2+
signals evoked by this molecule are often boos-
ted by Ca
2+
release by RyRs, InsP
3
Rs or both. Inter-
actions between different Ca
2+
release ... from [22].
M. Yamasaki et al. Calcium signalling by NAADP
FEBS Journal 272 (2005) 4598–4606 ª 2005 FEBS 4599
Ca
2+
release channels modulated by either InsP
3
or
cADPR that partici...