Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt

... 3413 NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-A u ) peptide complex from docking calculations Andreas ... D esign and synthesis of a p otent c yclic ana- logue of the myelin basic p rotein epitope MBP72-85: importance of the Al...

Ngày tải lên: 07/03/2014, 16:20

... added to the reaction. The protein amount of GST–Six1 was 16 ng for probe A (lanes 1–7), 15 ng for probe B (lanes 8–12) and 13 ng for probe C (lanes 13–17). The intensities of the retarded bands ... oligos 2, 3 and 6 for probe A, by oligos 6 and 7 for probe B and by oligos 6, 7 and 9 for probe C (Fig. 3B). In contrast, the formation of a complex by Si...

Ngày tải lên: 07/03/2014, 17:20

... with chloroform (20 mL), chloroform/methanol (98 : 2, v/v, 20 mL) and chloroform/methanol (1 : 3, v/v, 25 mL) [16]. In another experiment, total lipids from schizont forms were extracted and purified ... (pH 7.4). The mixture was extracted with chloroform/ methanol (1 : 1) and analysed by TLC in chloroform/methanol/water (65 : 25 : 2, v/v/v). Lanes 1, 2 and 3 are rings, trophozoite...

Ngày tải lên: 07/03/2014, 15:20

... NS2B–NS3pro, whereas the 3D NMR experi- ments of the complexes with 2 and 3 employed the WNV NS2B–NS3pro(K96A) mutant. The resonance assignments of the complexes with 1 and 3 were sup- ported ... spectra of combinatorially 15 N- labelled samples of WNV NS2B–NS3pro in the presence of 1. Fig. S4. 800 MHz 1D 1 H NMR spectra of the com- pounds 2 and 3 in the...

Ngày tải lên: 07/03/2014, 02:20

... alignment of eRF1. Fig. S11. The Ramachandran map plot (/ and w tor- sion angles for the protein backbone) of all 24 con- formers of the NMR families of solution structures of the closed and open conformers ... than 99% of the 1 H, 13 C and 15 N atoms of the protein backbone, and for more than 78% of the side chain atoms. The main set of bac...

Ngày tải lên: 06/03/2014, 11:20

... Structure of transformation plasmid pNICgfp and analysis of GFP expressing C. fusiformis transformants. (A) Restriction map of the part of plasmid pNICgfp containing the egfp gene flanked by the Pnr ... that the 5¢-UTR and 3¢-UTR of CfNR allow control of both the timing and level of expression of introduced genes in transgenic C. fusiformis. Results Increasing...

Ngày tải lên: 07/03/2014, 21:20

... designates the N-terminal transmembrane helix of the protein. On the basis of the three sets of secondary shift data in Fig. 3 (right), the helices probably range from I39 to E48 (helix 2) and from ... generates a Ramachandran plot for assessment of the geometric quality of protein conformers. The coordinates of the 20 lowest energy structures have been...

Ngày tải lên: 18/02/2014, 06:20

... aliquoted and stored at )80 °C. Peptides and nonpeptide ligands Chicken PYY and PP were ordered from Schafer-N (Copenhagen, Denmark). For the studies of Y 6 , pNPY and pPYY peptides were purchased from ... cloned and studied the tissue dis- tribution and phylogeny of the chicken Y 6 and Y 7 re- ceptors and performed the initial pharmacological characterizat...

Ngày tải lên: 19/02/2014, 07:20

... (Chlamydomonas sp. and Chlamydomonas reinhardtii) and all t hylakoid APXs from h igher plants analysed here. An important observation is the occurrence of two different APXs from the same organism (e.g. thylakoid ... i.e. the CCP line and the KatG fusion line, as is the c ase for Neuro spora crassa [47]. Evolution by the birth -and- death process in class I of t...

Ngày tải lên: 19/02/2014, 16:20

... 0.2 units of activity of the purified (lanes 1, 3 and 5) and recombinant (lanes 2, 4 and 6) enzymes were used. Lanes 1 and 2, E. quadrivirgata enzyme; lanes 3 and 4, E. climacophora enzyme; lanes ... far from amphibian enzymes, but close to mammalian and avian DNases I. Thermal stabilities of wild-type and substitution mutant snake DNases I The thermal stabilities of...

Ngày tải lên: 20/02/2014, 23:20