Báo cáo khoa học: Actin-binding domain of mouse plectin Crystal structure and binding to vimentin pot

... protein structures. J. Appl. Crystalogr. 24, 946–950. 1884 J. S ˇ evc ˇ ı ´ k et al.(Eur. J. Biochem. 271) Ó FEBS 2004 Actin -binding domain of mouse plectin Crystal structure and binding to vimentin Jozef ... difference of mouse and human ABDs per se. To document minor structural differences between human and mouse plectin ADBs, structure I molecule B (...

Ngày tải lên: 07/03/2014, 15:20

... etc.), and not to the subject of the clause. Hence, this distinction of forms, a vestige of erstwhile case marking, is not relevant to automatically distinguish uses of relative pronouns and is ... attempt to standardize as much as possible the set of features to be extracted. We exploit the full set of features and attributes available in the analysis, instead of...

Ngày tải lên: 31/03/2014, 20:20

... transcription of rat brain, F-11 and N18TG2 total RNA Total RNA of rat brain, F-11 and N18TG2 cells was prepared using TRIzol Reagent (Invitrogen) according to the instruction of the manufacturer. 1 lgtotalRNAwas incubated ... cells are present in F-11 cells, e.g. release of substance P, the presence of l-andd-opioid receptors, receptors for prostaglandin and bradykinin and...

Ngày tải lên: 31/03/2014, 07:20

... amount of oligomers, and this effect was used to investigate the details of both oligomer formation and structure by a combination of bio- physical techniques. The structural properties of the ... distribu- tion of 100 l M proinsulin C-peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C-peptide in the presence of i...

Ngày tải lên: 18/02/2014, 04:20

... lumen of the stomach, where both pepsinogen C and histone H2A are released, and there- after histone H2A would be processed into buforin-I and fixed to the mucous surface of epithelial cells to prevent ... histones H2B and H1, respectively. In the toad stomach [7], histones H1, H2A and H2B were observed in the cytoplasm of gastric gland cells along with pepsinogen C, wherea...

Ngày tải lên: 18/02/2014, 14:20

... upon binding to BchH may bring the integrin I domain of BchD into contact with the integrin -binding motif of BchH, simultaneously triggering porphyrin metallation. This would also lead to a release ... shown binding of Mg 2+ to this subunit [21]. An integrin I domain, suggested to exist in the C-terminus of the 70 kDa subunit, is a metal binding site (MIDAS motif)...

Ngày tải lên: 19/02/2014, 12:20

... upstream of the start codon of YEF1 (Table 5). The use of pET-DEST42 enabled us to fuse a V5 epitope and a His 6 tag to the C-terminal of Yef1p. E. coli BL21(DE3) was transformed with pET-YEF1 and ... unexpectedly found to be viable, for UTR1, YEF1 and POS5 demonstrated the critical contribution of Pos5p to mitochondrial function and survival at 37 °C and the criti...

Ngày tải lên: 20/02/2014, 01:20

... up of two to six protofilaments 2–5 nm in diameter with a cross-b-sheet spine [47,57] in which each polypeptide chain is structured into b-strands and each b-strand is arranged perpendicular to ... their soluble and monomeric forms [15,60–62] (see Figure 2). Aggregated structures are non-crystalline and insolu- ble and are therefore not amenable to X-ray crystallog- raphy and...

Ngày tải lên: 06/03/2014, 00:20

... limitation of PYM-based chemotherapy. The mechanism of cellular resistance to PYM is not fully understood, but it is extremely important to understand it for successful treatment of tongue carci- noma. ... induced by PYM, expecting to reveal new molecules related to PYM resistance, and to provide candidate biomarkers to predict the clinical response to PYM-based chemo- t...

Ngày tải lên: 06/03/2014, 22:21

... expression of olfactory receptors (ORs) is a primary require- ment to examine the molecular mechanisms of odorant perception and cod- ing. Functional expression of the rat I7 OR and its trafficking to ... control of FUS1 with the SSTR2 receptor and Gpa1. When this strain was incubated with either a-factor to stimulate endogenous a-factor receptor (Ste2), or with somatostati...

Ngày tải lên: 07/03/2014, 16:20