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Báo cáo khoa học: Actin-binding domain of mouse plectin Crystal structure and binding to vimentin pot

Báo cáo khoa học: Actin-binding domain of mouse plectin Crystal structure and binding to vimentin pot

Báo cáo khoa học: Actin-binding domain of mouse plectin Crystal structure and binding to vimentin pot

... protein structures. J. Appl.Crystalogr. 24, 946–950.1884 J. Sˇevcˇı´k et al.(Eur. J. Biochem. 271) Ó FEBS 2004 Actin -binding domain of mouse plectin Crystal structure and binding to vimentin Jozef ... difference of mouse and human ABDs per se. To document minor structural differences betweenhuman and mouse plectin ADBs, structure I molecule B(IB), structure II (II), and the structure of human plectin (HP) ... basis of their structuralsimilarity, the ABDs of fimbrin and plectin apparently alsohave a number of functions in common, including binding to vimentin. Similar to fimbrin [20] the ABD of plectin...
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Báo cáo khoa học:

Báo cáo khoa học: "The adaptation of a machine-learned sentence realization system to French" potx

... etc.), and not to the subject of the clause. Hence, this distinction of forms, a vestige of erstwhile case marking, isnot relevant to automatically distinguish uses of relative pronouns and is ... attempt to standardize as much as possiblethe set of features to be extracted. We exploit thefull set of features and attributes available in theanalysis, instead of pre-determining a small set of ... syntactic category of thenode, of its parent, the syntactic function of thenode, the voice of the parent, the arguments of the parent, and the agreement features of thegrand-parent. These features...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx

Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx

... transcription of rat brain, F-11 and N18TG2total RNATotal RNA of rat brain, F-11 and N18TG2 cells wasprepared using TRIzol Reagent (Invitrogen) according to the instruction of the manufacturer. 1 lgtotalRNAwasincubated ... cells are present in F-11 cells, e.g. release of substance P, the presence of l-andd-opioid receptors,receptors for prostaglandin and bradykinin and L-typecalcium channels. Recently, Bender ... threshold and it does not respond to capsaicin or protons. Here wedescribe the expression of VRL-1 in the rat dorsal rootganglion-derived cell line F-11, a hybridoma of mouse neuroblastoma (N18TG2) and...
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Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... amount of oligomers, and this effect was used to investigate thedetails of both oligomer formation and structure by a combination of bio-physical techniques. The structural properties of the ... distribu-tion of 100 lM proinsulin C-peptide in thepresence of divalent Ca2+ and Mg2+ionsunder native conditions (B), and of 100 lMC-peptide in the presence of insulin (C) and of 100 lM ... shifts back to higher wavenumbervalues, indicating the loss of b-sheet and the onset of a-helix structure formation. To visualize the trend inthe formation of b-sheet as a function of increasingSDS...
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Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt

Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt

... lumen of the stomach, where bothpepsinogen C and histone H2A are released, and there-after histone H2A would be processed into buforin-I and fixed to the mucous surface of epithelial cells to prevent ... histones H2B and H1, respectively. Inthe toad stomach [7], histones H1, H2A and H2B wereobserved in the cytoplasm of gastric gland cells alongwith pepsinogen C, whereas the N-terminal his-tone ... 3E,F).Time dependence of the bactericidal activity of H4-(86–100) and ciprofloxacinIn order to evaluate the rapidity of action of his-tone H4 peptides and compare it with that of thequinolone antibiotic...
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Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... upon binding to BchH may bring the integrin I domain of BchD into contactwith the integrin -binding motif of BchH, simultaneouslytriggering porphyrin metallation. This would also lead to arelease ... shown binding of Mg2+ to this subunit [21]. An integrin I domain, suggested to exist in the C-terminus of the 70 kDa subunit,is a metal binding site (MIDAS motif) that can be expected to bind ... D207N,R289K and L111F, and analysed their ability to interact withBchD. The ATPase-deficient BchI proteins provide a tool to dissect the interaction between BchI and BchD and rule outthe importance of...
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Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

... upstream of the start codon of YEF1(Table 5). The use of pET-DEST42 enabled us to fuse a V5epitope and a His6tag to the C-terminal of Yef1p. E. coliBL21(DE3) was transformed with pET-YEF1 and ... unexpectedly found to be viable, forUTR1, YEF1 and POS5 demonstrated the critical contribution of Pos5p to mitochondrial function and survival at 37 °C and the critical contribution of Utr1p to growth ... respectively. Km and Vmaxfor NAD and NADH of Pos5p have not been reported [2,3].Constructions of double and triple mutants forUTR1, YEF1 and POS5 To examine the roles of Yef1p, Utr1p and Pos5p,...
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Báo cáo khoa học: Biological role of bacterial inclusion bodies: a model for amyloid aggregation potx

Báo cáo khoa học: Biological role of bacterial inclusion bodies: a model for amyloid aggregation potx

... up of two to six protofilaments 2–5 nm in diameter with across-b-sheet spine [47,57] in which each polypeptidechain is structured into b-strands and each b-strand isarranged perpendicular to ... their soluble and monomericforms [15,60–62] (see Figure 2).Aggregated structures are non-crystalline and insolu-ble and are therefore not amenable to X-ray crystallog-raphy and solution NMR, ... path-ways to be tracked in real time and compounds able to block the self-assembly process to be identified [74].Finally, bacteria provide a means to trap and studythe highly toxic, unstable and transient...
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Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt

Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt

... limitation of PYM-based chemotherapy.The mechanism of cellular resistance to PYM is notfully understood, but it is extremely important to understand it for successful treatment of tongue carci-noma. ... inducedby PYM, expecting to reveal new molecules related to PYM resistance, and to provide candidate biomarkers to predict the clinical response to PYM-based chemo-therapy in tongue cancers.ResultsBiological ... K, Pastorek J, Wykoff CC, Gatter KC &Harris AL (2001) Hypoxia-regulated carbonicanhydrase-9 (CA9) relates to poor vascularization and resistance of squamous cell head and neck cancer to chemoradiotherapy....
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx

Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx

... expression of olfactory receptors (ORs) is a primary require-ment to examine the molecular mechanisms of odorant perception and cod-ing. Functional expression of the rat I7 OR and its trafficking to ... control of FUS1 with the SSTR2 receptor and Gpa1. When this strain was incubated with eithera-factor to stimulate endogenous a-factor receptor(Ste2), or with somatostatin 14 (S14) to stimulateSSTR2 ... Division of Food Sciences, School of Biosciences, University of Nottingham, Loughborough, Leicestershire, UKThe olfactory receptors (ORs) are a large group of proteins belonging to subfamily I of...
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