Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot

... Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus Zhanna Bugaytsova* and E. Bo¨ rje Lindstro¨m Department of Molecular Biology, Umea ˚ University, ... was satisfactory and that the tetrathionate hydrolase is a periplasmic enzyme. Purification of the tetrathionate hydrolase from tetrathionate- grown...

Ngày tải lên: 07/03/2014, 14:20

... GGGTCTAGAATGGAAAAAGATCTACAGTTAAGA 33 CCGGAATTCTTATTTCCCTTCTCTCATCTC 40 GCGCGCCATGGAAAAAGATCTACAGTTAAGA 41 GGGGGATCCCCATAATCCACTCCACCTGCTAAA 44 GGGGGGGATCCT CATTATTTCCCTTCT 66 AATACCGCCATGTATAATATCTATTACTTC 67 ... AATACCGCCATGTATAATATCTATTACTTC 67 GTAATAGATATTATACATGGCGGTATTGAA 75 GGGGGGGCCATGGAAAGAGCAGACGATTT 4294 H P. Chen et al.(Eur. J. Biochem. 271) Ó FEBS 2004 assay k it was obtained f ro...

Ngày tải lên: 30/03/2014, 15:20

... were purchased from Sigma, D -Val-Leu-Lys-pNA and D -Pro-Phe-Arg-pNA were from Serva. Glu-Gly-Arg-pNA, D -Ile-Pro-Arg-pNA, Bz-Phe-Val-Arg-pNA, D -Val-Leu- Arg-pNA and succinyl-Ala-Ala-Ala-pNA were obtained Correspondence ... kallikrein (Calbiochem, Affiliate of Merck, Darmstadt) were commercial preparations. The synthetic substrates N-succinyl-Ala-Ala-Pro- Phe-pNA and N -a- benzoyl...

Ngày tải lên: 31/03/2014, 01:20

... TGG AAC GAC GG gtaagggaac 172 ttttttttag A GCC ATA CTG Gly593 (2) 15 143 AAC AAA GCA G gtatattcag 434 atgtttccag GT GAG AGA ATG Gly640 (1) 16 156 CCG CCC CTT T gtaagcaacc 139 tcacctaaag CC AAA ... ttttctacag C ACC TTT ATT Ser331 (2) 9 80 AAC ACA AGG AA gtaagttcaa 535 tctgccacag T GAA AGC AGT Ans391 (2) 10 88 TTC AGG AAC ATG gtgagtgcct 306 atccttgtag TCC TTG AAG Met420 (3) 11 123 TTT CAA GTG...

Ngày tải lên: 31/03/2014, 07:20

... by glutamate-oxaloacetate t ransaminase (GOT) in the presence of a- ketoglutarate, and (c) formation of NAD + after malate dehydrogenase (MDH)-mediated reduction of oxaloacetate to m alate with NADH as cofactor. ... 3.5.1.1) are enzymes that catalyze the hydrolysis of L -asparagine to L -aspartate and a mmonia. Using amino acid sequences and biochemical properties as criter...

Ngày tải lên: 30/03/2014, 15:20

... the average error and the standard deviation. The largest average er- ror happens with the data annotated with multiple dialogue acts. In that case, the extracted segments have a starting and ... dialogue act labels in our dataset. The average number of words in utterances with only a single dialogue act is 7.5 (with a maximum of 34, and minimum of 1), and the average leng...

Ngày tải lên: 07/03/2014, 22:20

... (5¢fi3¢) FLAG epitope GAC TAC AAG GAT GAC GAT GAT AAG DYKDDDDK Library template (FLAG-negative) TTG GGG CTG GAT GAC GCG GAT AAG L GLDDADK Randomization NNS NNS NNS GAT GAC NNS GAT AAG XXXDDXDK Selected ... proteins carrying a functional FLAG epitope from an excess of FLAG-negative com- petitors and from a large library of random variants was also undertaken by plasmid display, to...

Ngày tải lên: 30/03/2014, 20:20

... cerevisiae MAP kinase cascades typically composed of three tiers of protein kinases, a MAP kinase (MAPK), a MAPK kinase (MAPKK) and a MAPKK kinase (MAPKKK), are common signalling modules in eukaryotic ... and Kholodenko et al. [19]. Among them, two recent remark- able developments are that of Kholodenko et al. [14] based on stationary experimental data, and that of Sontag...

Ngày tải lên: 07/03/2014, 21:20

... compo- nents of each reaction mixture, after their chromatographic separation and purification, were submitted to 1 HNMR (nuclear magnetic resonance) and FABMS (fast atom bombardment mass spectrum) analyses ... The concentration of both the assayed compound and its respective transformation product was determined by comparison of peak data with those obtained from authentic stan...

Ngày tải lên: 08/03/2014, 08:20

... 5¢-GCACAGTTCCCCTACAGTCCCGCTTTAG-3¢ HSCR ADI and G (up) 5¢-CCTTTTTAGCAGTGACTTTCCGTTGCAA-3¢ 5¢-CACGTCTCAGCAGGGAGAATAATCCCGA-3¢ Senescente associated prot ADI (up) 5¢-TTGCAACGACTGCAGTCATCAGTAGGGT-3¢ 5¢-GAGCTCAGCGAGGACGGAAACCTCGCGT-3¢ Lysosomalassociated protein ADI ... 5¢-GAGCTCAGCGAGGACGGAAACCTCGCGT-3¢ Lysosomalassociated protein ADI (up) 5¢-CCAATCAGGTAGGCCTTCATGGAGAGGA-3¢ 5¢-CCCAGAGATCCTCCAA...

Ngày tải lên: 30/03/2014, 15:20