Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

... 1055 Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase Nadthanan Pinchai, Bong-Seop Lee and Eggehard Holler Institut ... Among them, spheru- Fig. 2. Structural alignment of amino acid sequence by motifs, of spherulin 3b with spherulin 3a and other members of...

Ngày tải lên: 07/03/2014, 12:20

... that of Ce-RNH1a in the presence of Mg 2+ and about one-fortieth in the presence of Mn 2+ . The difference in specific activities between Ce-RNH1a and Ce-RNH1b suggests that the existence of the ... also determined the spe- cific activities of Ce-RNH1a and Ce-RNH1b in the presence of Mg 2+ or Mn 2+ ions, as shown in Table 2. Although the specific a...

Ngày tải lên: 30/03/2014, 11:20

... against polyhistidine revealed the same-sized bands, suggesting that the C-terminal histidine tag remained in both the proform and activated form in the recombinant enzyme after its secretion into ... to study the function of ADAMTSs during organogenesis in vivo. In this study, we isolated recombinant MIG-17 and determined the amino acid sequence of the N-ter- mi...

Ngày tải lên: 30/03/2014, 04:20

... cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min, 15.2 and 6.1 min, 6.8 and 4.2 min and 6.6 and 3.9 min respectively. The amount of purine or pyrimidine base formed is determined by integrating the ... mixture of 95% methanol and 0.1% trifluoroacetic acid in H 2 O. The retention times of adenosine and adenine, inosine and hyp- oxantine, guanosine and guanine,...

Ngày tải lên: 18/02/2014, 17:20

... bead characteristics, in which increases in molecular mass and DA resulted in efficient loading and minimization of the release of entrapped lipase [30]. The observed increase in specific activity with increasing ... 3600–3000 cm )1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement of both -OH… 3and -CH...

Ngày tải lên: 19/02/2014, 12:20

... physiological conditions, and confirmed to retain almost the same structure as that of the native protein. Both the protein from the adhesive layer of the barnacle and the recombinant protein were character- ized. ... characterize the nature of the protein in the primary cement. Thereaf- ter, we expressed the recombinant form of the protein in bacteria in...

Ngày tải lên: 07/03/2014, 05:20

... excitation) in the absence of calcium, and F s is the 510 nm emission signal (380 nm excitation) with a saturating concentration of calcium. Z1 and Z2 are the intrinsic fluorescence intensities of the ... in Krautwurst’s study [3], involving expression in HEK293 cells of a chimeric receptor including the N-terminus of rhodopsin and full-length I7 sequence and...

Ngày tải lên: 08/03/2014, 02:21

... re-replication of DNA in a single cell cycle. Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7]. Targets of the kinase in the regulation of DNA replication include ... dur- ing the HeLa cell cycle in a site -specific manner. Cyclin-dependent protein kinase is involved in the phosphorylation of MCM4 To determine w...

Ngày tải lên: 16/03/2014, 13:20

... did not result in any changes in the pattern of bands for either of the two cell types, showing that binding of C ⁄ EBP and FoxA to the CPS GRU in FTO-2B cells is independ- ent of these hormonal ... the lower strand. FoxA binding resulted in protection of the gua- nines at positions 347 and 351 on the upper strand, and protection at position 352 on the lo...

Ngày tải lên: 23/03/2014, 10:20

... key substrates of casein kinase I (CKI). However, the specific sites phosphorylated in vivo by CKI and their relative import- ance in the physiologic regulation of these proteins in the canonical ... phosphor- ylated in vivo. Casein kinase I epsilon (CKIe) is a positive regulator of the Wnt signaling pathway downstream of Wnt and upstream of Dvl, axin and b-ca...

Ngày tải lên: 30/03/2014, 10:20