Báo cáo khoa học: WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Cf pptx

... both proteins, Flag- VAMP2 and HA-PKCf. These findings raised the question how ProF would affect the interaction between VAMP2 and PKCf.In order to test this, we expressed Flag -VAMP2 and HA-PKCf ... 90, 8392–8396. WD-FYVE protein interacts with VAMP2 and PKCf T. Fritzius et al. 1566 FEBS Journal 274 (2007) 1552–1566 ª 2007 The Authors Journal compilation ª 2007 FEBS WD-repea...

Ngày tải lên: 07/03/2014, 10:20

... glycogen synthase and mitogen-activated protein kinase by insulin in rat adipocytes: comparison of insulin and protein kinase C modulators. Biochem Biophys Res Commun 209, 1082–1088. 7 Von Willebrand M, ... of guanosine triphosphatases and phospholipase C-gamma2 in extracellular signal-regulated kinase, c-Jun NH 2 -terminal kinase, and p38 mitogen-activated protein kinase...

Ngày tải lên: 30/03/2014, 20:20

... dynamics and oncogenic Ras signaling Insights from FAS, FAS ligand and RASSF1A Samir K. Patra 1, * and Moshe Szyf 2 1 Cancer Epigenetics Research, Kalyani, India 2 Department of Pharmacology and Therapeutics, ... nucleotide-bind- ing protein, which plays a role in the regulation of growth and differentiation in eukaryotic cells [1,82]. Despite profound improvements in our underst...

Ngày tải lên: 18/02/2014, 14:20

... protein m isfolding diseases. Nature 416, 507–511. 3. Dobson, C .M. ( 2003) Protein folding and misfolding. Nature 426, 884–890. 4. Stefani, M. & Dobson, C.M. (2003) Protein aggregation and aggregate ... glycine, and ethylene glycol, have been used [12], these are not enough t o solve the problems of protein aggregation and misfolding. Recently we reported that polyamines,...

Ngày tải lên: 19/02/2014, 16:20

... a control for protein transfer and (Cb) for protein loading. In (C-b), (i) and (ii) represent normal and tumor muscle homogenate respectively; (iii) and (iv) repre- sent normal and tumor mitochondria ... creatine kinase and sarcomeric mitochondrial creatine kinase showed that both of these isoforms decreased as malignancy progressed. Expressions of mRNA of muscle-type crea...

Ngày tải lên: 07/03/2014, 06:20

... L-BFGS algorithm (Nocedal and Wright, 1999). And finally, we show that the parsing algo- rithm described in Clark and Curran (2003) is ex- tremely slow in some cases, and suggest an efficient alternative ... for estimation, and resulting in an extremely efficient parser. This paper assumes a basic understanding of CCG; see Steedman (2000) for an introduction, and Clark et al. (2002)...

Ngày tải lên: 23/03/2014, 19:20

... (lanes 2 and 4) and 32 P-labeled ETS-1 (lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B) EMSA was per- formed with EWS (lanes 2, 4 and 6) and 32 P-labeled Htelo (lanes 3 and 4), dsHtelo (lanes 5 and ... RRM–RGG2–ZnF (lane 4) and RGG3 (lane 5). EMSA was performed with these proteins and 32 P-labeled Htelo. (C) EMSA was performed with RGG3 (lanes 2 and 4) and 32 P-labeled...

Ngày tải lên: 15/02/2014, 01:20

... residue yeast protein involved in the coordination of cell wall synthesis and cell growth [18], the nucleoprotein and phosphoprotein from measles and Sendai viruses [19,20] and the Rhabdoviridae phosphoprotein ... n 2 Þ where [h] 1 and [h] 2 correspond to the measured mean ellip- ticity values per residue of proteins 1 and 2, respectively, and n 1 and n 2 correspond to the n...

Ngày tải lên: 18/02/2014, 04:20

... cysteine dioxygenase (CDO) (Protein Data Bank identifier: 2ATF) [20], ARD (Protein Data Bank identifier: 1VR3) [21], QDO (Protein Data Bank identifiers: 1Y3T and 1JUH) [11,12], gentisate 1,2-dioxygenase (Protein Data ... 2D40 and 3BU7) [22,23], and b-diketone-cleaving dioxygenase (Dke1) (Protein Data Bank identifier: 3BAL) [24]. Pirins are nuclear proteins that also contain a three-h...

Ngày tải lên: 18/02/2014, 06:20

... least four serines. In rat HSL, Ser563, Ser659 and Ser660 are phosphorylated by protein kinase A (PKA) in vitro as well as in vivo, and Ser660 and Ser659 have been shown to be the activity-controlling sites ... bis-ANS and HSL. The concentrations of bis-ANS used were 1.5 l M and 2.0 lM in (A) and (B), respectively. HSL was phosphorylated with 15 l M ATP in the reaction mix, a...

Ngày tải lên: 18/02/2014, 11:20