Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc

... FEBS 1035 Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica Nobuhiro Ikezawa 1 , Kinuko Iwasa 2 and Fumihiko ... 38557–38565], no information is available regarding the genes for methylenedioxy bridge-forming enzymes in stylopine biosynthesis. Two cyto- chrom...

Ngày tải lên: 07/03/2014, 10:20

... processing), and GmPDIL-1 and GmPDIL-2 associate with proglycinin and b-con- glycinin in the ER, suggesting that they may play important roles in folding and in formation and rearrangement of disulfide ... synthesis levels of both b-conglycinin and glycinin, as pro-b-conglycinin and proglycinin are transient pro- tein forms that are present in the ER prior to process-...

Ngày tải lên: 18/02/2014, 11:20

... GmPDIL-1 and GmPDIL-2 func- tion in the folding of both proglycinin and b-conglyci- nin a¢-subunit. Alternatively, GmPDIL-1 and GmPDIL-2 may be involved in the degradation of unfolded glycinin and ... or GmPDIL-2 and proglycinin or b-conglycinin during the course of the folding process of these proteins. Results Cloning and expression of GmPDIL-1 and GmPDIL-2 T...

Ngày tải lên: 07/03/2014, 05:20

... roots of the thoracic ganglia and in the subesophagic ganglion of Homarus americanus [15,16]. It is also detected in the retina of the crayfish Procambarus clarkii [17]. The cloning and molecular characterization ... not in muscle or stomach. We also inves- tigated the ability of dsRNA to inhibit the CHH function in shrimps in vivo. Injection of CHH dsRNA into the...

Ngày tải lên: 23/03/2014, 10:20

... Kirakosyan, A., Inoue, K., Hiraoka, N., Ikeshiro, Y., Kushiro, T., Shibuya, M. & Ebizuka, Y. (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin ... Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica , involved in biosynthesis of br...

Ngày tải lên: 08/03/2014, 23:20

... shift of the peak in 209 nm. The calcium binding and conformational changes of oyster CaLP and CaM were further investigated by monitoring intrinsic phenylalanine and tyrosine fluor- escence. Intrinsic ... Comparison of the amino- acid composition of the calcium-binding domains in canonical EF-hands [30] with that in the EF-hands in oyster CaLP, reveals a good correlation...

Ngày tải lên: 16/03/2014, 23:20

... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein inter- action domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger ... FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved in the induction of dimer...

Ngày tải lên: 16/02/2014, 09:20

... thermotolerance and insensitivity to inhibition by downstream products, cit- rulline and arginine. Results The enzyme involved in catalysis of the fifth step of citrulline biosynthesis in wild watermelon leaves During ... [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32]. In plants, information on the localization o...

Ngày tải lên: 20/02/2014, 03:20

... indicated by solid thin lines (b 1 ) andshadedthinlines(b 1B ), and the unidenti- fied 3¢ untranslated region of b 1B is indicated using the thin interrupted broken line. The stop codon is indicated by ... UÆmL )1 ;Gibco)inCa 2+ -free medium, followed by rinsing and storage in saline medium containing Ca 2+ and 50 lgÆmL )1 gentamicin. Stage V–VI oocytes were separated for injection t...

Ngày tải lên: 16/03/2014, 23:20

... larvae and by tail vein injection of 200 lL, subcutaneous injection of 2 mL, or intracerebroventricular Ó FEBS 2002 Molecular cloning and function of gene BmKIM (Eur. J. Biochem. 269) 4469 injection ... lepidopterous and dipterous insects [8]. The excitatory toxins cause a fast excitatory paralysis in animals and induce repetitive firing in insect nerves; in contrast, t...

Ngày tải lên: 31/03/2014, 09:20