Báo cáo khoa học: Transient potential receptor channel 4 controls thrombospondin-1 secretion and angiogenesis in renal cell carcinoma ppt

... compilation ª 2007 FEBS 6377 Transient potential receptor channel 4 controls thrombospondin-1 secretion and angiogenesis in renal cell carcinoma Dorina Veliceasa1, Marina Ivanovic2, Frank Thilo-Schulze ... 12917–12922. 24 Adams JC (20 04) Functions of the conserved thrombo-spondin carboxy-terminal cassette in cell extracellularmatrix interactions and signaling. Int J Biochem Cell Biol 36, 1102–11 14. 25 Dinser ... mimicking the RCC phenotype. Furtheranalysis revealed a profound decrease in transient receptor potential canon-ical ion channel 4 (TRPC4) Ca2+ channel expression in RCC cells. TRPC4silencing in...

Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... pump and in vitro vacuolar acidiﬁcation in hepatoma HepG2 cells. The vacuolar H+-ATPase inhibitor baﬁlomycin and the cathepsin D inhibitor pepstatin Apartially inhibited, both in vivo and in ... in NaCl ⁄ Pifor 4 min and 0.5% saponine in NaCl ⁄ Pifor 10 min. Permeabilized cellswere blocked for 20 min with 10% horse serum in NaCl ⁄ Pi, and then incubated for 60 min with a rabbit ... 1–30 min.cAMP assaysFollowing injection of native CT, rats were sacriﬁced at50 min and livers were rapidly removed and minced in cold ethanol containing 1 mm isobutylmethylxanthine (40 mL...

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... binding of ACP tothe docking site followed by a conformational change in ACP proposed to inject the acyl chain into the sub-strate-binding pocket [32]. If a similar mechanism isinvolved in ... protein was crystallized in hanging drop experiments at 2 94 K with reservoirs contain-ing 2% (w ⁄ v) poly(ethylene glycol) 40 0, 100 mm bis-Trispropane (pH 6.5) and 1.9 m (NH 4 )2SO 4 (resulting ... but can bea cysteine (in E. coli KAS II [21,22]) or an asparagine (in Mycobacterium tuberculosis and Mycobacteriumleprae KAS I and II [23, 24] ).Scheme 1.Histidines and lysine in KAS I ⁄ KAS II...

Tài liệu Báo cáo khóa học: Non-speciﬁc depolymerization of chitosan by pronase and characterization of the resultant products pptx

... in the initial hour,which could be due to prior attainment of an activeconformation by the enzyme, remaining stable up to 240 min (4 h) after which there was a decline (Fig. 4C,D),indicating ... radiation at 1. 541 4 nm (Enraf Nonius Co., Bohemin,NY). The relative intensity was recorded in a scatteringrange (2h)of0 45 °. The crystallinity index (CrI, %) wasdetermined using the formula ... 3600–3000 cm)1 in the spectra of bothchitosan and LMWC indicated the absence of free -OHgroups and the involvement of both -OH… 3and -CH2OH…6 in intra- and intermolecular hydrogen bonding(Fig....

Tài liệu Báo cáo khoa học: "Discriminative Lexicon Adaptation for Improved Character Accuracy – A New Direction in Chinese Language Modeling" pptx

... be used in the construction.That is, beginning with only characters in the lexi-con and using the training data to alter the currentlexicon in each iteration. This is also an interestingdirection.ReferencesMaximilian ... Brill, and Andreas Stolcke. 2000.Finding consensus in speech recognition: Word er-ror minimization and other applications of confusionnetworks. Comp. Speech Lang., 14( 2):373 40 0.Andrew Y. Ng and ... chinese documents. In COLING,pages 169–175.Berlin Chen, Jen-Wei Kuo, and Wen-Hung Tsai. 20 04. Lightly supervised and data-driven approaches tomandarin broadcast news transcription. In ICASSP,pages...

Báo cáo khoa học: Proteomic analysis reveals Hrs ubiquitin-interacting motif-mediated ubiquitin signaling in multiple cellular processes ppt

... UIM-mediated ubiquitin signaling in cells, weperformed a proteomic screen for Hrs UIM-interacting ubiquitinated pro-teins in human brain by using an in vitro expression cloning screeningapproach. ... Proteins can be either monoubiquitinatedor polyubiquitinated by attachment of a multiubiquitinchain linked through one of the internal lysine residues in ubiquitin [2]. K48-linked polyubiquitination ... degradativeprotein sorting by scaffolding Hrs in dynamic microdo-mains. J Cell Sci 119, 241 4– 242 4.59 Toonen RF & Verhage M (2007) Munc18-1 in secre-tion: lonely Munc joins SNARE team and takescontrol....

Báo cáo khoa học: "Using Leading Text for News Summaries: Evaluation Results and Implications for Commercial Summarization Applications" ppt

... documents in order to include the logical lead in most instances. Using a fixed amount of leading text regardless of document length would have resulted in LEADs that include 13 64 too much ... queries to the headline and leading text than if they were to apply their queries to the full text. Documents in our news database have several text fields including HEADLINE and BODY fields. ... documents that contain "Clinton" in the LEAD and "budget" any- where in the document. Customers who use LEAD routinely combine it with the HEADLINE field. We tested...

Báo cáo khoa học: Bovine tryptases cDNA cloning, tissue speciﬁc expression and characterization of the lung isoform ppt

... 62–76 230– 245 1576.0 1576.3 113–126 149 0.7 149 0.5 167–180 144 2.5 144 2.1 88–1001366.2 1365.7 188–2011 344 .5 1 344 .4 150–1611330.8 1330 .4 77–871272 .4 1271.9 88–991263.7 1263.1 139– 149 1217.5 ... Clin. Invest. 88, 49 3 49 9.17. Stack, M.S. & Johnson, D.A. (19 94) Human mast cell tryptaseactivates single chain urinary-type plasminogen activator(pro-urokinase). J. Biol. Chem. 269, 941 6– 941 9.18. ... were con-ducted using 40 pmol of each primer, under the followingconditions: 2 min at 94 °C (1 cycle), 1 min at 94 °C, 1 minat 58 °C, 1 min at 72 °C (30 cycles) and 10 min at 72 °C(1 cycle).Evaluation...

Báo cáo khoa học: On peptide bond formation, translocation, nascent protein progression and the regulatory properties of ribosomes ppt

... nascent chains that can affect protein elongation in prokaryotes and in mammalian cells [67–71], and act as adiscriminating gate. Striking examples are SecM, a proteinbelonging to the secretion ... cross-linking data [44 ] and rationalizing the difﬁculties of its localization [18,23 ,44 ,45 ]. In accordance with the ﬁnding that despite sparsomycinuniversality, ribosomes from various kingdoms ... participating in erythromycin binding [75], in line with cryo-EM studies [76] and conﬁrming ourproposal that erythromycin resistance is due to indirecteffects [9]. Further support for the linkage...

Báo cáo khoa học: Human liver mitochondrial cytochrome P450 2D6 – individual variations and implications in drug metabolism docx

... ﬂuoro-graphy. Transient transfection of WT and mutant CYP2D6 in COS-7 cellsCOS-7 cells were cultured in DMEM containing 10% fetalbovine serum and gentamycin (50 lgÆmL)1). Cells weretransiently ... in CYP2D6 content, ranging from a relatively high level in HL1 34 and 137 to a moderate level in HL136, lowlevels in HL132, 139 and 140 , and almost undetectablelevels in HL131 and 141 (Fig. 1A). Densitometry ... protein in the binding to receptor sites. EMBO J 6, 344 9– 345 4.25 Blom N, Sicheritz-Ponten T, Gupta R, Gammeltoft S &Brunak S. (20 04) Prediction of post-translational glyco-sylation and...

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