Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx

... destabilizing the double bond character of the pThr-Pro bond [26]. Further evidence is available for the catalytic mecha- nism of Pin1 resembling more closely that of the other prolyl cis ⁄ trans isomerases, ... molecular mechanisms of the enzyme action. Most importantly, we want to critically review the evidence that Pin1 would or would not act as a pro...

Ngày tải lên: 07/03/2014, 05:20

... tation of the x MG P promo ter constru cts used for transient transfections o f A6 cells is shown to the left. T he nomenclature of the promoter deletions is based o n the transcription start of the xMGP ... the molecular organization of the first nonmammalian M GP gene and the functional analysis of its promoter. We identified a region within the first 70 bp of...

Ngày tải lên: 08/03/2014, 10:20

... amino group at carbon 2 of the guanine ring may interact with the side chain of Glu309, whereas the carbonyl group at position 6 of the guanine ring may interact with the hydroxyl group of the Tyr239 side ... structures of the EhPGK obtained using the modeller program (Fig. S1), it was found that the only difference in the amino acids that bind the purine ring...

Ngày tải lên: 16/03/2014, 01:20

... which the majority of the protein motion is defined [27,28]. The analysis is based on the diagonalization of the covariance matrix built from the atomic fluctuations after the removal of the A B Fig. ... that defines the surface of the gap region [26]. In the interior of the HPV-16 b-barrel, at the inter- face of each dimer, only one small cavity, present fo...

Ngày tải lên: 16/03/2014, 10:20

... in the spleen of these mice. For the determination of the number of PMM56 bacteria present in the spleen of these four mice, we thus chose a value of 50 CFU per spleen. The average number of ... deciphering the complex biosynthetic pathways of DIMs and PGLs. These efforts have led to the identification of more than 20 proteins required either for the formation o...

Ngày tải lên: 23/03/2014, 09:20

... by the structure of EcR [20]. Therefore, the elucidation of EcR and USP (RXR) structures is important for understanding the molecular mechanism of the action of 20E. In this study, we report the ... regions. The C-regions of EcRs and USPs are highly conserved. However, other regions, particularly the N-terminal parts of USP ⁄ RXR, vary. The alignments of am...

Ngày tải lên: 30/03/2014, 04:20

... mm Na 2 HPO 4 ⁄ 1.8 mm KH 2 PO 4 ⁄ 137 mm NaCl ⁄ 2.7 mm KCl ⁄ 1mm CaCl 2 ⁄ 2mm MgCl 2, pH 7.3 (NaCl ⁄ P i ). The cells were resuspended in 10 mm Tris ⁄ HCl (pH 8.0) ⁄ 150 mm NaCl ⁄ 1mm EDTA containing ... fur- ther the specificity of the interaction, native cardosin B was used in the binding assays. Despite the high simi- larity between the two...

Ngày tải lên: 30/03/2014, 11:20

... constant (K d ) of 4.8 · 10 )7 M. The thermal unfolding of the heterodimer was completely reversible, with a T m of 48 °C. The comparative thermodynamics and thermal unfolding analysis of the Rv3619c–Rv3620c ... determination of thermodynamic parame- ters. The parameters used in fitting were the stoichiom- etry of association (n), the binding constant (K b ), and the...

Ngày tải lên: 22/03/2014, 16:21

... members of the VOC superfamily. Superimposing P. shermanii MCE on the human glyoxalase structure shows that the Co 2+ ion of the MCE is only 0.2 A ˚ from the position of the Zn 2+ ion in the glyoxalase ... involved the insertion of several fragments of the 5¢-region of mce-1 into the mul- tiple cloning site of the vector pPD95.77 provided by A. Fire (Carneg...

Ngày tải lên: 23/03/2014, 13:20

... the sku phenotype. Because G376V was a novel mutation in IVD, the effect of the mutation was further investi- gated by molecular modelling. Together with the previ- ously reported traits of the ... 4452–4463 ª 2010 The Authors Journal compilation ª 2010 FEBS 4453 the a-hydrogen of the substrate, and the other is G374, which forms the binding pocket for the branch...

Ngày tải lên: 18/02/2014, 04:20