Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf

... FEBS 4787 Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus Zhi-Qiang Wang 1 , Yu-Hong Han 1,2 , Xiao-Xia Shao 1 , Cheng-Wu Chi 1,2 and Zhan-Yun ... homology with protein disulfide isomerases from other species, and 70% homology with human protein disulfide isomerase. Enzymatic analyses of recombi- nant...

Ngày tải lên: 07/03/2014, 05:20

... Dolichols of rubber plant, ginkgo and pine. Phytochemistry 51, 11–15. 4680 K. Asawatreratanakul et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Molecular cloning, expression and characterization of cDNA encoding cis -prenyltransferases ... HRT2 protein catalyzes the synthesis of new rubber molecules with the activeinvolvementofanumberoffactorsinWBPoffresh Hevea latex. Materials...

Ngày tải lên: 07/03/2014, 21:20

... lane 0, protein standards. (C) Protein bands after affinity chromatography and renaturing process. Lanes 1 and 2, protein bands after separation by affinity column; lanes 3 and 4, protein bands after ... fusion protein was renatured and examined by SDS ⁄ PAGE gel (Fig. 5C), indicating a clear and unique protein band of 21.7 kDa, which matched well with the theoretical ma...

Ngày tải lên: 28/03/2014, 23:20

... as an inhibitor of protein disulfide isomerase (PDI) to demonstrate the role of the protein- folding catalyst in a variety of molecular pathways. Commercial bacitracin is a mixture of at least 22 ... cyclic peptide; protein disulfide isomerase; protein disulfide isomerase (PDI) inhibition; substrate-binding domain; thiol disulfide exchange Correspondence S. McCormick,...

Ngày tải lên: 14/03/2014, 23:20

... isolation and characterization of the STAT5 gene from T. fluviatilis (TfSTAT5), the third member of the STAT family from fish, to establish the evolutionary relationship of STAT5 between mammals and ... phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription. EMBO J. 13, 4361–4369. 20. Akira, S. (1999) Functional roles of...

Ngày tải lên: 31/03/2014, 07:20

... structures of EcR and USP from L. decemlineata (b) the analysis of mRNA expression profile of L. decemlineata, EcR and USP, and (c) the measurement of the binding affinity of steroidal and nonsteroidal ... 4119 and L. migratoria USP showed higher homology to RXR of human and mouse than USP isoforms of dip- teran and lepidopteran insects. Thus, the functions of...

Ngày tải lên: 07/03/2014, 21:20

... found to be a tetrameric protein with a molecular mass of 220 kDa, whereas native BHAb was found to be a dimeric protein of 120 kDa. Protein characterization using LC-MS ⁄ MS and sequence alignment ... PAGE with apparent molecular mass values of 60 kDa and 120 kDa (Fig. 3B). Similarly, the purified protein BHAb was eluted from Superdex 200 (data not shown) Fig. 2. Eff...

Ngày tải lên: 18/02/2014, 14:20

... mass of a given particle is subjected to error if it deviates from the shape and solvation of the molecules used as standards (globular proteins). The molecular mass for a protein is estimated from ... local environment of Tyr63 and/ or a contribution of the binding of the copper to the thiolates of the protein. A plot of the intensity of the signal at 265 nm again...

Ngày tải lên: 23/03/2014, 12:20

... Coexpression, purification and characterization of the E and S subunits of coenzyme B 12 and B 6 dependent Clostridium sticklandii D -ornithine aminomutase in Escherichia ... nto the same expression vector under the control of the T7 promoter, and coexpression of oraE and oraS genes was carried out in E. co li. Meanwhile, the extent of the involvement of AdoCbl or ......

Ngày tải lên: 30/03/2014, 15:20

... obtained from the Protein and Nucleic Acid Chemistry Facility, Department of Biochemis- try, University of Cambridge and, in the case of primer Lib2.for, from Gibco BRL (Paisley, UK). Details of all primers ... selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase Wayne M. Patrick 1,2 and Jonathan M. Blackburn 1,3,4 1 Departm...

Ngày tải lên: 30/03/2014, 20:20