Báo cáo khoa học: Binase cleaves cellular noncoding RNAs and affects coding mRNAs potx

... FEBS Binase cleaves cellular noncoding RNAs and affects coding mRNAs Vladimir A. Mitkevich 1 , Nickolai A. Tchurikov 1 , Pavel V. Zelenikhin 2 , Irina Yu. Petrushanko 1 , Alexander A. Makarov 1 and ... 5K RNase Sa and RNase Sa. As for binase, less than 1% of the label was revealed in this region for both ssRNA and dsRNA samples. Binase affects the quantity of proa...

Ngày tải lên: 06/03/2014, 09:22

... ranked list (Luhn 1958). Figures 2D, E and F show the standard deviation be- tween the document and sentence space, the document and term space and the sentence and term space respectively. These ... sentence, and term, spaces in our corpora of more than one-hundred thousand full-text docu- ments; Section 4 discusses the results; and Sec- tion 5 draws conclusions from this...

Ngày tải lên: 17/03/2014, 04:20

... Journal compilation ª 2006 FEBS 5053 and cholesterol metabolism are shown in Fig. 3B. For example, mRNAs coding for phosphatide phosphatase (EC 3.1.3.4) and apoliprotein C-II, an essential co- factor ... glycolysis and gluconeogenesis, fatty acid degradation, cholesterol and bile acid metabolism, amino acid degra- dation and ammonia utilization. In addition, several enzymes of p...

Ngày tải lên: 23/03/2014, 10:20

... Mecky Pohlschro ¨ der, Philip Rea and Carsten Sanders for many valuable discussions and help. This work was supported by grants DOE 91ER20052 and NIH GM38237 to F. D. and by grant BMBF-LPD 9901/8-14 ... system [5]. TatE is a structural and functional homolog of TatA and encoded at a different locus [6]. TatA and TatB together can form a double-layered ring structure and are su...

Ngày tải lên: 23/03/2014, 21:20

... at 23 °C, and the protein concentration was kept constant at 6 l M . The excitation and emission bandwidths were 5 and 3 nm, respectively. The excitation wavelength was set at 286 nm, and the spectra ... from Azotobacter vinelandii were studied by fluorescence and NMR spectroscopy. The catalytic Cys230 residue of the enzyme was selectively labelled using [ 15 N]Cys, and changes in...

Ngày tải lên: 31/03/2014, 07:20

... H 2 O 2 and hydroxyl radicals. Increased ROS concentration inside a cell results in damage to the main biomole- cules and membranes and essential metabolic functions [1]; to maintain a low intracellular ... 42 in the N-terminal region. Expression and purification of Bcp1, Bcp3 and Bcp4 bcp1, bcp3 and bcp4 were amplified by PCR from S. solfataricus genomic DNA and cloned into pET-...

Ngày tải lên: 07/03/2014, 05:20

... investigated both in isolated and in cellular complexes. For this purpose, constitu- tive and immune-isolated proteasomes and two human glioblastoma cell lines, U87 MG and T98G, differing in their ... of lactacystin (0, 2.5, 5 and 10 lm). Then, TQ (5, 20 and 100 lm) was added to the mixtures and incubated for 1 h at 37 °C. Samples were then resolved by 12% SDS ⁄ PAGE and ele...

Ngày tải lên: 15/03/2014, 11:20

... cdc37-681 [24], cdc37-184, cdc37-13 and cdc37-J (E. L. Turnbull and P. A. Fantes, unpublished observations). Medium (minimal medium and yeast extract medium) for standard growth con- ditions was used ... Chris Prodromou and Kazuhiro Shiozaki for reagents. Thank you to Kate Belaya and Paul Westwood for their valu- able contributions to this work, Adrian Bird and Robert Klose for us...

Ngày tải lên: 16/03/2014, 22:20

... loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK Alamdar Hussain 1,2, *, Liang Yu 1,3, *, Rani Faryal 1,2 , Dara K. Mohammad 1 , Abdalla J. Mohamed 1,4 and C. I. Edvard ... metazoans [1] and com- prise five members in mammals: Bruton’s tyrosine kinase (BTK), inducible T-cell kinase (ITK), TEC, BMX [also known as epithelial and endothelial tyro- s...

Ngày tải lên: 14/02/2014, 18:20

... 917–924. 133 Fernandez Madrid F (2005) Autoantibodies in breast cancer sera: candidate biomarkers and reporters of tumorigenesis. Cancer Lett 230, 187–198. a-enolase in tumor diagnosis and therapy ... therapeutic and diagnostic tumor target Michela Capello, Sammy Ferri-Borgogno, Paola Cappello and Francesco Novelli Department of Medicine and Experimental Oncology, Center for Experim...

Ngày tải lên: 14/02/2014, 19:20