Báo cáo khoa học: Calmodulin-mediated regulation of the epidermal growth factor receptor doc

... [72]. The binding of these proteins could prevent the interaction of the Ca2+⁄ CaM complex to the receptor if the CaM-BD were occluded at least in part. Nevertheless, the docking of these ... activation of the EGFR, where the C-terminal lobe of the kinase domain of one of the monomers forming the dimeric receptor interacts with the N-terminal lobe of the apposed monomer[19,47,84]. Of relevance ... thatcould account for the Ca2+⁄ calmodulin-mediated regulation of epidermal growth factor receptor activity. The control exerted by calmodulin ondistinct epidermal growth factor receptor- mediated...

Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc

... interesting that the )10 sequence islocated inside the iron box of desA (nucleotides 7–12 of the iron box), explaining the regulation of desAexpression by binding of DmdR1 to the iron box. The same ... at24 h. The coordinated regulation by iron of the expres-sion of the entire cluster ensures simple and efﬁcientup -regulation of desferrioxamine biosynthesis afteriron limitation. The two upstream ... hwas observed, but no further growth occurred there-after. The RT-PCR analysis revealed induction of the tes-ted desA and desD genes (located at the beginning andend of the cluster) under iron-limiting...

Báo cáo khoa học: Mechanical regulation of the Cyr61/CCN1 and CTGF/CCN2 proteins Implications in mechanical stress-associated pathologies pptx

... by the cells results in profound alterations of the histo-morphometry, phenotype and function of the cells[3–7]. The onset of this process is characterized by the activation of a cascade of ... not clear whetherthere are any speciﬁc regulators at the receptor level of signal transmission, or whether targeting of Cyr61 andCTGF is achieved by speciﬁc cofactors at the level of cellular ... expression?Mechanistically, the transfer of excessive strain resultsin the activation of multiple signaling cascades, cul-minating in the reprogramming of gene expressionand the production of growth factors such...

Tài liệu Báo cáo khoa học: Evolutionary relationships of the prolyl oligopeptidase family enzymes docx

... ancestor of life. With respect to the studied enzymes of the POP family, POP seems to be the most conserved enzyme. Ten conserved amino acids werefound at the active site of the enzyme of each of the ... proposed that the b-propeller of P. furiosus POP does not perform the same function as the mammalian enzyme, i.e. the exclusion of large peptidesfrom the active site [25]. Clearly the role of the b-propellerhas ... b4-strand and the aB¢-helix at the active site, and Gly511 is the ﬁrst residue of that a-helix. The high degree of conservation of theseresidues suggests that this turn between the secondarystructure...

Báo cáo khóa học: Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase docx

... process, the assignment of the cofactors involved was possible froma thorough analysis of the voltammetric responses and the structural properties of the molecular surface of DgAOR.Many factors ... the logarithm of CVcathodic and anodic peak currents with the logarithm of the scan rate; (B) variation in the CV cathodic and anodic peak potentials with the logarithm of the scan rate of the ... on a goldelectrode and in the presence of neomycin, the redoxreaction observed is due to the reduction of the Mo atom of the molybdopterin cofactor. Indeed, although the molyb-denum site is...

Báo cáo khoa học: Phosphorylation of Hrs downstream of the epidermal growth factor receptor docx

... role in the traﬃcking of growth factor/ receptor complexes through early endosomes. Stimulation of cells with epidermal growth factor (EGF) rapidly leads tophosphorylation of Hrs, raising the question ... serum-starvedandstimulatedwithEGForTGFa before the cells werewashed and Hrs was immunoprecipitated from the lysate.We chose to stimulate the cells with the growth factorsfor 8 min, as the growth factor- stimulated ... whether the receptor tyrosine kinase phosphorylates Hrs directly. Here,we present evidence that a downstream kinase, rather than the active receptor kinase is responsible. We show that the nonreceptor...

Tài liệu Báo cáo khoa học: Redox regulation of dimerization of the receptor proteintyrosine phosphatases RPTPa, LAR, RPTPl and CD45 pdf

... conditions. The ectodo-mains of both monomers in the dimer are required forthis effect, suggesting that the epitope tag of onemonomer is masked by the ectodomain of the othermonomer in the dimer, ... of oxidation of the catalytic site cysteines. Takentogether, these limited tryptic proteolysis suggest thatoxidation induced a change in conformation of the intracellular domain of this panel of RPTPs.A ... GST-LAR(Fig. 2D). The tryptic cleavage sites were localizedthroughout the cytoplasmic domains of these fourRPTPs. One site was conserved in three of the fourRPTPs at the )5 position relative to the TyrTrpPro-motif....

Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx

... 6). On the otherhand, knockdown of either HO-1 or HO-2 expressionresulted in the accumulation of heme in the hemin-treated HepG2 cells. Taken together with the siHO-2-mediated induction of HO-1 ... The intracellular concentration of hemeis maintained by the rate of its synthesis and degrada-tion [1]. Many enzymes and their regulators areresponsible for heme synthesis [1,2]. On the otherhand, ... and HO-2 mRNA. The intensity of the signals representing HO-1 or HO-2 mRNA was normalized with respect to the intensity of the 18S rRNA signal. The ratio of each normalized value to the control...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala ... increase in the mutantmice, the disruption of DEC1 might be compensated byDEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The suppressive activities of DEC1 and ... connected to the DEC1 E-boxes(Fig. 2), while deletion of the Orange domain ( DEC1DOr-ange) had no effect on the suppressive activity, indicating the importance of t he bHLH region of DEC1. The HXXXXXXXR...

Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx

... another site, so that the phosphorylation kinetics of one residue can depend on the phosphorylation state of other residues in the pro-tein [8]. It is not clear how these factors modulate the response ... modiﬁcations of proteins are the rule ratherthan the exception [3,4]. Proteins phosphorylated atseveral sites include, for example, membrane receptors,such as epidermal growth factor receptor ... in the previous sections for the special case of weak binding of the enzymes,holds more generally.Individual regulation of the phosphorylation sitesSo far, we have focused our analysis on the...

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