Báo cáo khoa học: The oleic acid complexes of proteolytic fragments of a-lactalbumin display apoptotic activity pdf

... display cellular toxicity (not shown). The extent of apoptotic activity of the OA complexes of the fragments was comparable to that observed with the OA complex of the intact protein prepared by chromatography, ... [4]. The OA complexes of the two fragments, as obtained by mixing them with 15 equivalents of the fatty acid, displayed significant apopto...

Ngày tải lên: 06/03/2014, 09:22

... proteins; M pep , the number of unique matched peptides; U pep , the number of unmatched peptides in the MALDI-TOF experiments. The function of new proteins is based on the GH family of the catalytic ... theoretical sum of the activi- ties of the individual fractions measured independently (at half or one third of the protein concentration). The dark grey bar...

Ngày tải lên: 18/02/2014, 08:20

... terms of the size of the TatC-containing substrate-binding com- plex. Some of the difference may stem from the absence of a TatB component, but the complexes may well contain differing numbers of ... smaller than the TatAdCd complex (350 kDa by gel-filtration chromatography) [21]. These size estimates are influenced by the size of the detergent micelle and the tr...

Ngày tải lên: 16/03/2014, 04:20

... allow binding of TPP to E1. The assay, in a final volume of 1 mL, was started by the addition of the 2-oxoacid substrate as for the assay of E1, and OADHC activity was monitored by measuring the production ... the decarboxylation of the branched-chain 2-oxoacids and pyruvate [14]. In the current paper, we report the clon- ing and expression of the E2 and E3 ge...

Ngày tải lên: 16/03/2014, 05:20

... (1968), the latter of whom defined this approach as the Distribu- tional Hypothesis: for two words, their similarity in meaning is predicted by the similarity of the distributions of their co-occurring ... construct the vector space from word fre- quencies in the documents. The documents are defined independently of the words that appear in them. Co-occurrence models bui...

Ngày tải lên: 17/03/2014, 00:20

... the EGF domain and six in the CFC domain. To test whether the CFC domain could retain its function independent of the EGF domain, we generated a soluble form of the CFC domain, comprised of the ... signi- ficance of the CFC domain. In the present work, we have solved the disulfide structure of the CFC domain and have conducted biochemical studies that detail the...

Ngày tải lên: 23/03/2014, 21:20

... recognized the amino-terminal Ala23–Val28 loop of L-PGDS, revealed that both the mRNA and the intact form of L-PGDS were local- ized in the cells of Henle’s loop and the glomeruli of the kidney, ... minor immunoreactivity of cathepsin B was not colocalized with MAb-10A5 immunoreactivity, but the majority of these two immunoreactivities was colocalized to the same o...

Ngày tải lên: 30/03/2014, 01:20

... entirely to the catalytic role of these domains. Rather, the advantage appears to depend on the ability of the lipoyl group to protrude from the inner core of the complex, indicative of the biological ... Another essential feature of the lipoate residues within the 2-oxo acid dehydrogenase complexes arises from the ÔcrowdingÕ effect. Confinement of the com...

Ngày tải lên: 17/03/2014, 09:20

... of the presence of ribosomal proteins in the TIAR-TAP pellet is difficult to address because ribosomal proteins are common contaminants of TAP assays [43,44]. On the other hand, the presence of ... interactions. The capacity of ASF ⁄ SF2 to down-regulate mRNA expression was further confirmed by artificial tethering of a reporter mRNA to the 3¢ UTR. In this assay, the do...

Ngày tải lên: 16/02/2014, 15:20

... excluded the possibility of cell leakage and the release of proteins or intracellular glu- cose under NaF treatment. Further validation of our assay was the successful measurement of acid trehalase activity ... together may constitute the catalytic domain of Ath1 [22]. To map the minimal domain of Ath1 that allows the secretion of this protein, various DNA fragm...

Ngày tải lên: 18/02/2014, 06:20