Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc

... CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site Karen Duus 1 , Nicole M. Thielens 2 , Monique ... both CD91 and the lectins. The interaction site of the CD91 molecule on MBL ⁄ L-fico- lin seemed to be in close proximity to the MASP-asso- ciation site because inhibition was observed with the MA...

Ngày tải lên: 06/03/2014, 01:23

... homologue. The murine Nbr1 gene liesheadtoheadwiththeBrca1 gene, with the intragenic region of 289 bp suggesting the possibility of co-ordinated expression of the two genes. Mutations in the human ... heavily O-glycosylated protein c orrelate with the expected p roperties of the polypeptide carrying the CA125 epitope [4]. NBR1 shares little h omology with other k nown pr...

Ngày tải lên: 08/03/2014, 10:20

... blocking, the cells were incubated with 3% bovine serum albumin in NaCl/P i for 1 h. Then, they were incubated with the anti-HA (3F10) or NF-jB-p65 antibody (F-6) overnight at room temperature, and the ... NF-jB forms a complex with the IjBs and is thereby locked into the cytoplasm. IjBa is the best-characterized IjB, and is also a target of NF-jB itself. Thus, we focused on...

Ngày tải lên: 16/03/2014, 04:20

... tail. There is an unbroken reading frame of 162 amino acids that is continuous with the vector GAL4DBD and terminates with TGA (Fig. 2A) at nucleotide 487. Inspection of the sequence shows that the ... pro- teins and the results are shown in Fig. 3. These con- firm that UXT binds Rp (Fig. 3B, lane 5) and furthermore also interacts with full-length Evi1 (Fig. 3B, lane 7). Format...

Ngày tải lên: 16/03/2014, 11:20

... sites. Gal-Jun(1±223) contains the Gal4 DNA-binding domain fused to the transactivation domain o f Jun. The pCMVnlac encodes the sequences for the b-galactosidase gene under the control o f the ... dematin and therefore suggests a novel mechanism f or linking the Ras signaling complex to the actin cytoskeleton. The functional signi®cance of t he dematin interaction with...

Ngày tải lên: 17/03/2014, 11:20

... 3F: lane 4) with the RNA ladder (Fig. 3F: lane 1) suggest that the IMP1 binding site of the ARS falls within the nucleotide sequence shown in the DARS-R. We further investigated the ability of ... binding the RNA could alter their binding site on the ARS. To understand the molecular nature of the interac- tion of IMP1 with the ARS and PABP, we examined the IMP1...

Ngày tải lên: 23/03/2014, 10:20

... concentrated in the nucleolus, whereas the C-terminal portion of Nop53p might interact with the proteins in the pre-60S complex, but is less concentrated in the nucleolus. These results raised the question ... confirming the specificity of this interaction. These results led to the conclusion that, by binding to the 5.8S rRNA and through its interaction with Rrp6p, Nop53...

Ngày tải lên: 30/03/2014, 04:20

... shown). We therefore assume that either the extraction conditions were inadequate for the iso- lation of the natural product, or that the gene clus- ter is silent under the conditions employed. The irrevocable ... carbon of the serine and the d-CH 2 of the hOrn, which constitutes the DKP. Therefore, putting all these long-range connections together, we established a stru...

Ngày tải lên: 16/02/2014, 09:20

... resi- dues, all facing the inside of the cell. Five of the residues lie within the previously characterized N-terminal regulatory domain and two mutations are located within the approach to the first transmembrane ... electrostatic interactions with other nearby lysine residues. This, in turn, is likely to reduce the flexibility of the section linking the N-terminal regulato...

Ngày tải lên: 07/03/2014, 15:20

... XhoI(ATACTCG AGCTCGTCTTCACCGAG) sites at the 5¢ and 3¢ ends of the gene, respectively. The resulting gene was ligated into the pET-24b(+) vector (Novogen, Nottingham, UK) between the NdeIandXhoIsitestoyieldthe plasmid ... reflected in Fig. 5A, the proteinase K treatment had no effect on the ability of either FtsY or the FtsY NG domain to bind to the membrane, with floatation...

Ngày tải lên: 16/03/2014, 16:20