Báo cáo khoa học: Acceleration of disulfide-coupled protein folding using glutathione derivatives potx

... Acceleration of disulfide-coupled protein folding using glutathione derivatives Masaki Okumura 1,2 , Masatoshi Saiki 2,3 , Hiroshi Yamaguchi 1 and Yuji Hidaka 2 1 School of Science ... true when high protein concentrations were employed, whereas folding recovery using ECR was similar to that of the glutathione redox system. Kinetic analyses of the oxidative foldi...

Ngày tải lên: 06/03/2014, 00:21

... optimization of the positions of the hydrogen atoms using energy minimi- zation; (c) optimization of the conformation of the side chain using energy minimization; and (d) optimization of the posi- tions of ... truncated by using a cutoff of 12.0 A ˚ . The initial structure for the MD simulation was constructed by the following four steps: (a) optimization of the position...

Ngày tải lên: 16/03/2014, 06:20

... analysis of proteins was performed by automated Edman degradation using a Perkin-Elmer Procise 494-HT protein sequencer with reagents and methods of the manufacturer. Oligomerization of the undenatured ... reveals the protein regularly accumulating over an expression period of 3 days, while only traces of other proteins were detected. After dialysis of culture supernatant, t...

Ngày tải lên: 21/02/2014, 03:20

... by heterotrimeric G proteins regulates a wide variety of physiological functions. We are interested in the manipulation of G -protein- mediating signal transduction using G -protein- coupled receptors, which ... affected by manipula- tion of the GPCR–G protein signal transduction. Our interest is in the use of GPCRs derived from evolutio- narily distant organisms for the manipulat...

Ngày tải lên: 07/03/2014, 11:20

... constituents of one clause bear certain kind of structural relationship to those of the other. Although there are an infinite number of situations, there seems to be only a small number of properties ... can cover wider range of in- stances of re-linkage. The result of the extension is shown in Table 2 (for cases each of which shares a subject) and Table 3 (for cases...

Ngày tải lên: 08/03/2014, 05:21

... indicate beginning of injection of the extract, downward arrows indicate beginning of washing. An aglycin interacting protein is present in the pancreatic extract. Fig. 5. ELISA of the purified protein from ... coelution of plant and animal material in RP-HPLC and by electrophoresis [26]. Structural analysis of aglycin Molecular masses of components recovered from RP-HPLC wer...

Ngày tải lên: 23/03/2014, 09:21

... consistent with our concept of the unfolding region [9,10]. According to this concept, unfolding of a protein molecule starts at its weakest site, and local stabiliza- tion of this unfolding region results ... chevron plots of unfolding of pWT (Figs 4 and 5), which indicates that unfolding occurs in more than one step [29–31]. Reduction of autoproteolysis, in the presence of 2...

Ngày tải lên: 19/02/2014, 17:20

... disulfide bridge has a profound effect on the structure of TNSALP (Fig. 10). The effects of substitution of R433 either with alanine or aspartate on the catalytic properties of TNSALP were reported by ... V max values suggests that the catalytic efficiency of the mutant protein is dramatically reduced com- pared with that of the wild-type. Increased suscepti- bility of TNSALP(R4...

Ngày tải lên: 16/03/2014, 12:20

... Pgp-mediated efflux of TMRM (Fig. 7). On the other hand, the anesthetics benzyl alcohol, chloroform and propofol accelerated the efflux of TMRM (Fig. 7). Lack of correlation between acceleration of drug permeation ... efflux of preloaded TMRM was due mainly to acceleration of passive transport of the dye. In this case, the efflux is due to Pgp activity and passive transport of...

Ngày tải lên: 30/03/2014, 04:20

... 1 Scatter plot of number of medication prescribing errors (MPEs) at screening day according to number of drug orders per patient (24 hour screening day)Scatter plot of number of medication prescribing ... one unit of our ICU, we implemented an ICIS with incorpo- rated CPOE and a moderate level of CDSS. The objective of this study was to evaluate and compare the incidence and s...

Ngày tải lên: 25/10/2012, 10:39