... with[35S]methionine may indicate an alteration in the rate of its degradation during the labeling period. In fact,only 15% of 35S-labeled mutant protein remains (vs.100% of wild-type protein at 0 ... h). Nevertheless, itcould also be explained by changes in translation ormaturation of the protein. An increase was alsoobserved in the rate of degradation of the remainingmutant protein (20% ... is more consistent with an increaseddestabilization of the protein in the cytoplasm, a pro-cess that, in the case of the Gqfamily of proteins, isindependent of receptor activation. Short-term...