Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx
Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx
... Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid Masaya Ohta1, Y. T. Pan2, ... Chemistry,Louisiana State University, Baton Rouge, LA, USAA series of trehalose-based oligosaccharides were isolated from the cytoplasmic fraction of Mycobacterium smegmatis and purified by gel-filtration ... microlitre of the matrix solution and 1 lLof the peracetylated oligosaccharides in chloroform weremixed on the sample plate directly and dried to avoidFig. 1. Separation of the cytosolic oligosaccharides...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... phosphoenolpyruvate (PEP) carboxykinasecatalyzes the decarboxylation of oxaloacetate and transfer of the c-phosphoryl group of ATP to yield PEP, ADP, andCO2. The interaction of the enzyme with the ... significant increase of the contribution of the slowcomponent. This result points to changes in the dynamicalproperties of the local environment of the P-pyridoxylgroup due to changes of the protein ... coordination to two water molecules, an oxygenatom from Pc of ATP, Ne2 from His232, an oxygen atom from the side chain of Asp269, and Ne from Lys213.Ó FEBS 2002 Ligand interactions of PEP carboxykinase...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... data from ref [11].c[31].d[36].eglycylglycylhistamine, [9].fa-hydroxymetylseryl-a-hydroxymetylserylhistidinamide; Cu(II) data from [37]; Ni(II) data from [38].gN-Terminal 15-peptide of ... Asp-Ala-His-NH2(DAHam)wasagiftofHenrykKozlowski,Faculty of Chemistry, University of Wroclaw. Stocksolutions of NiCl2and CuCl2were standardized gravimet-rically with dimethylglyoxime and complexometrically withEDTA, ... Bal1,21Faculty of Chemistry, University of Wroclaw, Poland;2Institute of Biochemistry and Biophysics, Polish Academy of Sciences,Warsaw, PolandA comparative study of thermodynamic and kinetic...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... present in the apical membranes of the oxyntic-peptic cells [37]. The distribution of the ecto-ATPDase on these epithelial cells is distinctly different from theotherATPDaseintheE-ATPasefamily,theCD39s[13,17,19].Molecular ... ecto-ATPDasesare nearly identical with respect to their enzymatic propertiesand mass of the deglycosylated proteins. The primarysequence of the liver ecto-ATPDase deduced from its cDNAobtained by RT-PCR ... shown to be responsiblefor the E-ATPase activity of intact rat hepatocytes [40].Thattheecto-ATPDaseisthemajorE-ATPaseinliverswas supported by our recent study of the E-ATPase activityin the...
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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx
... of the CD95 from inside the hepatocyte to the plasma mem-brane [39], which depends on a ROS-mediated tyrosinephosphorylation of the epidermal growth factor(EGF)-receptor, the association of ... association of CD95 with the EGF-receptor, and phosphorylation of CD95 onTyr232 and Tyr291 by the EGF-receptor tyrosinekinase activity [15]. Although the appearance of CD95at the plasma membrane ... severe hyperosmolarity (‡ 505 mosmolÆL)1) shifts the balance towards hepatocyte apoptosis [15].Like hyperosmolarity, CD95 ligand in hepatocytesvia generation of ROS induced EGF-receptor tyrosinephosphorylation,...
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Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf
... stoichiometry [28]. The uncertainty layin the number of ion-translocating subunits in the membrane-embedded rotor. Recently, the atomic struc-ture of a c ring from the F1F0ATP synthase from I ... both types of c subunits. The stoichiometry for c2⁄ c3: c1was9 : 1. Furthermore, this stoichiometry was independent of the carbonsource of the growth medium. These analyses clearly demonstrate, ... is to energize endo-cytoplasmatic membranes in the eukaryotic cell [13]. The Na+F1F0ATP synthase operon from the anaerobic, acetogenic bacterium Acetobacterium woodiidiffers from all other...
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Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf
... in vivo. The reason why the cleavage band was not observed in this assay may be the rapid degradation of the purified protein from the cell lysate. When subjected to a longer exposure, the blot ... shifted the equilib-rium of the protein from cytoskeletal to soluble cytosolic pools, and alteredgreen fluorescent protein-tagged s-DAPK-1 protein localization as observedby confocal microscopy. These ... proteinsolubility, s-DAPK was found to localize in both the ‘insoluble’ cytoskeletal and soluble cytosolic fractions(Fig. 5B), whereas the s-DAPK-1Dtail equilibrium wasshifted more into the soluble cytosolic...
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Tài liệu Báo cáo khoa học: Pathways and products for the metabolism of vitamin D3 by cytochrome P450scc docx
... abstraction of a hydrogenatom and insertion of the hydroxyl group. Thus,C17 must be able to come close to the heme iron,presumably due to the side chain occupying aslightly different position in the ... hydroxylate vitamin D3 to produce20-hydroxyvitamin D3 and other poorly characterized hydroxylated prod-ucts. The present study aimed to identify all the products of vitamin D3metabolism by ... that the trihydroxyvitamin D3 can bemade from 20,23-dihydroxyvitamin D3 and thus pro-vides the sites of two of the three hydroxyl groupsadded to vitamin D3 by P450scc.Identification of dihydroxyvitamin...
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Tài liệu Báo cáo khoa học: "An Experiment in Evaluating the Quality of Translations" pdf
... Russian they might have to divine the meaning of the translations. The rankings of the translations by the average rat- ings on the informativeness scale are almost precisely complementary to the ... adequacy of the target sentences rated by the monolinguals cannot be resolved from the pres- ent experiment. Because it was desired to preserve the symmetry of Parts I and II of the experiment, the ... intelligibility, on the average, than do the monolingual raters; this is probably to be explained on the basis of the instructions to the Russian readers, which were to use any ingenuity or knowledge of...
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Tài liệu Báo cáo khoa học: Fatty acid desaturases from the microalga Thalassiosira pseudonana pptx
... was then cloned into the corresponding sitesbehind the galactose-inducible GAL1 promoter of pYES2(Invitrogen) to yield the plasmids pYDESA, pYDESB, pY-DESI, pYDESO, and pYDESK. The fidelity of ... into the vector pYES2, under the control of aninducible galactose promoter, to produce the constructspYDESA, pYDESB, pYDESI, pYDESK and pYDES-O. They were first expressed in the Saccharomyces ... analysis of FAMEs from yeast transformed with the empty plasmid pYES2 or the plasmid containing TpDESK. Yeastcells transformed with either pYDESK (top chromatogram) orpYES2 (bottom chromatogram)...
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