Tài liệu Báo cáo khoa học: Medium-chain dehydrogenases/reductases (MDR) Family characterizations including genome comparisons and active site modelling pdf

... characterizations including genome comparisons and active site modelling Erik Nordling 1,2 , Hans Jo¨ rnvall 1 and Bengt Persson 1,2 1 Department of Medical Biochemistry and Biophysics and 2 Stockholm ... The LTD active sites all are deep and narrow (Table 2). The active site is polar in all cases but one, and for the majority of the LTD members, several charge...

Ngày tải lên: 21/02/2014, 03:20

... is the same in both proteins, including a short segment (N56, C57 and D58) forming a turn of 3 10 helix (D75, C76 and D77 in a-sarcin). The active site The active site is composed of well-defined ... acid and a base on each side of the hydrolyzed bond [16]. E96 and H137 in a-sarcin and E58 and H92 in RNase T1 act as the base and acid groups, respectively. Com- paring th...

Ngày tải lên: 18/02/2014, 08:20

... KIND and PTPN13 KIND (Fig. 6A). The amino acid sequences of the core regions of mouse, human and Gallus v-KIND shared 65.6% homology, and 17 identical and four function- ally similar residues, including ... dendrites and soma, although not to axons. The expression of three other KIND2-containing constructs (DKIND1, DRasN and DGEF) was restricted to dendrites and soma (Fig. 1B)...

Ngày tải lên: 14/02/2014, 19:20

... several phosphorylated sites, including Thr69 and Ser87, and its degradation is promoted through dephosphorylation of these sites. Ser70 is the major physiological phosphorylation site for the survival function ... phosphorylation of the MAPK family and mitochondrial proteins. Phosphorylated ERK prevents the activation of caspases and the Bcl-2 family, whereas these are activat...

Ngày tải lên: 14/02/2014, 22:20

... homeostasis disorders [6]. With this method, it is possible to mutate the mouse genome randomly on a large scale, and then isolate and identify the mutated gene. Several other genes have been identified ... this mutant strain as described below). Body and organ weights as well as plasma parameters (Tables S2 and S3) were measured, and learning, mem- ory and behavioral tests (Tabl...

Ngày tải lên: 16/02/2014, 09:20

... Green PCR Core Kit (Bioke, Leiden, The Netherlands), 3 pmol of each primer (Isogen, De Meern, The Netherlands or Biolegio, Nijmegen, The Netherlands) and 3 lL of cDNA template (equivalent to 1 ng ... work of BM Bakker and HV Westerhoff is further supported by STW, NGI-Kluyver Centre, NWO-SysMO, BBSRC (including SysMO), EPSRC, AstraZeneca, and EU grants BioSim, NucSys, ECMOAN, and Un...

Ngày tải lên: 18/02/2014, 06:20

... & Latchman DS (1999) Signal transducer and activator of transcrip- tion-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90beta gene promoters. J Biol Chem ... the basis of their apparent molecular weight and function, such as Hsp105 ⁄ 110, Hsp90, Hsp70, Hsp60, Hsp40, and Hsp27. The Hsp70 family is the major and best-characterized gro...

Ngày tải lên: 18/02/2014, 06:20

... a (encoded by CKA1) and a¢ (encoded by CKA2), and two regulatory b subunits, b and b¢ in Saccharomyces cerevisiae (CKB1 and CKB2). The enzyme occurs in all possible combinations of a and b subunits ... cycle and highly resistant to proteolytic degradation [40]. Miscellaneous procedures Yeast were grown on standard media [41] and transformed as described by Gietz et al. [42]. Mic...

Ngày tải lên: 18/02/2014, 16:20

... stimula- tory and inhibitory G protein and predominant localiza- tion of the endogenous CT receptor ganglioside GM1 [28,29]. This would facilitate interactions between CT, GM1 and Gsa and, potentially, ... various other tissues and cells has established both a cytosolic and membrane-bound localization [38], and over- expressed ARF-6 has been localized to the plasma membrane and...

Ngày tải lên: 19/02/2014, 02:20

... with methylglyoxal and diacetyl. In the oxidative reaction, the enzyme was active with glycerol and to a lower Table 1. The specificities and kinetic properties of the histidine-tagged and purified GLD1 and GLD2. ... and gld2, were cloned and expressed in a heterologous host. The encoded proteins were purified and their kinetic properties characterized. GLD1 catalyses the conve...

Ngày tải lên: 19/02/2014, 06:20