Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx

... Functional expression and mutational analysis of flavonol synthase from Citrus unshiu Frank Wellmann 1, *, Richard Lukac ˇ in 1, *, Takaya Moriguchi 2 , Lothar Britsch 3 , Emile Schiltz 4 and ... been functionally proved and the lability of substrate and product rule out any cocrys- tallization. The setting of a helices and b-strands causes very similar CD s...

Ngày tải lên: 21/02/2014, 03:20

... The copy number of each variant in the unknown samples was determined from the calibration curves and data were normalized to the copy number of 18S RNA. Expression of ACMSD isoforms in E. coli pGEM-ACMSD ... software swiss-pdbviewer and visual molecular dynamics [49]. Acknowledgements We thank G. Magni and S. Ruggieri for helpful com- ments and suggestions and T. Begley (...

Ngày tải lên: 19/02/2014, 02:20

... a k cat of 7.7 ± 0.9 s )1 , K m of 0.148 ± 0.028 mm and k cat ⁄ K m of 5.2 · 10 4 m )1 Æs )1 toward shikimate, and a k cat of 7.1 ± 0.7 s )1 , K m of 0.182 ± 0.027 mm and k cat ⁄ K m of 3.9 · ... shikimate, k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and K m of 2.9 mm toward NAD. The optimum pH of the enzyme activity is between 8.0 and...

Ngày tải lên: 19/02/2014, 05:20

... Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai Ken-ichi Suzuki, Takao Ojima and Kiyoyoshi Nishita Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries ... phosphate (pH 7.0), and an appropriate amount of enzyme at 30 °C. The reducing sugar liberated by hydrolysis of CMC was determined by the method of Nelson and So...

Ngày tải lên: 20/02/2014, 23:20

... fluorescence intensity as function of time of double- labeled liposomes made from PS (A) or from PC (B) after the addition of MfGl-IIorLPSReatt = 50 s and subsequent addition of LBP (0.2 m M )att = 100 ... were kind gifts of K H. Wiesmu ¨ ller 1 (Tu ¨ bingen, Germany). Bovine brain 3-sn-PS and egg 3-sn-PC were obtained from Sigma (Deisenhofen, Germany). For pre- paration o...

Ngày tải lên: 21/02/2014, 00:20

... the activation of Ras and ERK1 ⁄ 2 downstream of GPCRs, Ras-GRF1 could be one of possibly several GEFs involved in the activation of Ras and subse- quently ERK1 ⁄ 2 downstream of G s -coupled ... Research Council of Norway, The Novo Nor- disk Foundation, Anders Jahre’s Foundation for the Promotion of Science, The Blix Family Foundation and grants from the University of...

Ngày tải lên: 19/02/2014, 18:20

... been isolated from the digestive glands of scallops collected in Japan, and these are stereoisomers of PTX-1 and PTX-6, respectively. After acid treatment of PTX-4 and PTX-7, two isomers, named PTX-8 and ... interactions leads to a cessation of new filament growth and a disruption of existing filaments, and is accompanied by a loss of motility, breakdown of adherens...

Ngày tải lên: 18/02/2014, 14:20

... graph of R 1 , R 2 and { 1 H}– 15 N NOE val- ues along the sequence of the TTF1 HD at 11.7 T and 286 K. Fig. S2. Plot of J(0.87x N )–J(0) correlation from 15 N relaxation measurements of the ... and Ala60–Gln67), together with those from Arg58 and Gln59 in the C-terminal end of helix III, are located apart from the major cluster, towards the lower intercept of the th...

Ngày tải lên: 18/02/2014, 16:20

... Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. It ... system [48]. The visual analysis of cono- marphin was done using molmol [49] software, and the geometric qualities of the obtained structures were assessed with procheck-nmr soft...

Ngày tải lên: 18/02/2014, 17:20

... nature of the molecule, as assessed from the salt and heparin dependence of the antimicro- bial and antifungal effects. Results Antimicrobial effects of recombinant wild-type trappin-2 and trappin-2 ... (2006) Secretion of serine peptidase by a clinical strain of Candida albicans: influence of growth conditions and cleavage of human serum proteins and extracellular ma...

Ngày tải lên: 18/02/2014, 17:20