Tài liệu Báo cáo khoa học: Structure of peptidase T from Salmonella typhimurium doc

... outside these regions, and the unique tripeptidase speci®city of peptidase T, suggested that the structure of the two enzymes would in part differ. We report the three-dimensional structure of ... CPA. The similarity between the structure of t he second domain in CG2 and peptidase T extends to the interchain contacts around the twofold crystallographic axis of the two...

Ngày tải lên: 21/02/2014, 03:20

... active site of RNase Sa2 contributed to a better understanding of enzyme–substrate recognition. Results Description of the structures Crystal structures of RNase Sa2 with a free active site (3D5G) ... Dinucleotides (B) and trinucleotides (C) with highest scoring rates docked into the active site of RNase Sa2. The trinucleotides are grouped into two clusters that differ in the positi...

Ngày tải lên: 18/02/2014, 11:20

... spectrometer. The pattern of the over- laid spectra is almost identical to that of the full-length construct, showing that the two domains of At3g16450.1 are largely inde- pendent. Fig. 5. Investigation ... solution structure of the 32 kDa At3g16450.1 protein from A. thaliana by the SAIL-NMR method. This is the first application of SAIL-NMR in a structural genomics study. It pro-...

Ngày tải lên: 18/02/2014, 14:20

... primer 5¢-TTTT TCTAGA CTA TGT CTT CAT GTC GAC GTC-3¢. The underlined portions of the primers represent the inserted BamHI and XbaI restriction sites, respectively. The vector pcDNA3.1+ and the LdEcRA ... 5¢-TTTT GAATTC AAG CGG GAG GCG GTT CAA GAA-3¢ (which lies just to the 3¢-side of the DBD-encoding sequence). Each primer was paired with the reverse primer 5¢-TTTT AAGCTT CTA AGT ATC CGA...

Ngày tải lên: 18/02/2014, 08:20

... in the main chain of the subunit, or in the interaction of subunits within the trimer that linked the conformation of residues 120–125 to the position of the active site lip and closure of the ... 4189 deaminase structures (Figs 1B, 2 and 3). Interestingly, the crystal structure of the other inactive mutant enzyme H121A in complex with dCTP was very similar to the structure o...

Ngày tải lên: 18/02/2014, 16:20

... steady-state concentrations ( [T] ¼ [T* ]) and the two complex con- centrations are equal ([TK] ¼ [T* P]). Therefore, the total target concentration can be expressed as: T T ¼ 2 [T] +2[TK]. After substitution of the ... target protein, the sequestered fraction increases, which cau- ses the sensitivity to decrease. In Fig. 2C the activated fraction of the target T* is plotted, illustrat...

Ngày tải lên: 19/02/2014, 07:20

... 5¢-GGCAGCACGGCTCCG GACAGGGGCTATGGCGTCC-3¢. HIV-1 integrase oligonucleotide substrates The following HIV-1 substrates were used. HIV U5 LTR (+) strand: 5¢-ATGTGGAAAATCTCTAGCA(GT)-3¢, (–) strand 5¢-ACTGCTAGAGATTTTCCACAT-3¢. Non-viral ... at determination of the crystal structure of at least the isolated domains of M-PMV integrase, and possibly of the complete protein. Materials and me...

Ngày tải lên: 19/02/2014, 16:20

... (sentreq "sentstat nil) ; 2 (message "foc in "top < ;t& gt; "tim <> nil "subjclass <sc>) ; 3 (message "foc nil "top < ;t& gt; "tim <> ... of the first module is to perform the arithmetic computation required to produce facts that contain the relevant information needed to infer interest- ing messages, and to write those fa...

Ngày tải lên: 21/02/2014, 20:20

... also suggested that some of the reports of general nuclease sensitivity might in fact be attributable to the hyper- sensitivity at the DHSs within these active chromatin domains. It was once thought that ... multi- ple factors that cooperate in the destabilization of chromatin at specific sites located close together. Part of this cooperativity comes from the fact that some transcrip...

Ngày tải lên: 14/02/2014, 18:20

... indicate that the D-loop does not have stable contact with the N-terminal domain. The relative orientation of the core structure of the N-terminal domain to the Rossmann fold domain and the anticodon-binding ... the D-loop of tRNA Arg are not important for the binding of tRNA Arg on ArgRS in the aminoacylation reaction. The crystal structure of free ArgRS from T. thermophi- l...

Ngày tải lên: 18/02/2014, 11:20