Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf

... physicochemical and biological properties of DM64, an antimyotoxic protein from opossum serum. DM64 is an acidic protein showing 15% glycosylation and with a molecular mass of 63 659 Da when analysed ... equimolecular amount of DM43 to the enzymes. Statistical analysis Results represent mean ± SEM (n ‡ 4). Data were statis- tically evaluated by Analysis of Varianc...

Ngày tải lên: 21/02/2014, 01:21

... 269) Ó FEBS 2002 Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana Oligomerization and thermotolerance Julie A. Crack, Marc Mansour, Yu Sun and Thomas H. MacRae Department ... ordinary hydration and temperature, cell maintenance entails a constant and substantial free energy flow [51,52]. Anoxic cysts m ay acquire s ufficient energy to survive b...

Ngày tải lên: 22/02/2014, 04:20

... reporter assay. Western blot analysis Rabbit antibodies to DEC1 were produced by immun- izisation with the synthetic peptide fragment Cys-Lys- Gly-Asp-Leu-Arg-Ser-Glu-Gln-Pro-Tyr-Phe-Lys-Ser- Asp-His-Gly-Arg-Arg. ... the left-hand panel. Expression of FLAG-DEC1 was examined by Western blot analysis with anti- FLAG immunoglobulin (right panel). (C) Expression levels of DEC1 and BMA...

Ngày tải lên: 19/02/2014, 16:20

... the recovery in LPL activity after cotransfection of S132T and WWW mutants. The expression levels of wild-type and mutant LPLs were detected by Western blotting with anti-(His- tag) polyclonal antibody ... Trp394) (C,D). Protein expression of the wild type, the WW mutant, and the WWW mutant tagged with polyhistidine were evaluated by Western blotting with anti-(His-tag) polyclon...

Ngày tải lên: 21/02/2014, 15:20

... associated with dopamine treatment and modulated by a-synuclein overexpression, without any a priori hypothesis [4,11,12]. SH-SY 5Y cells couple good dopamine transporter activity with a low activity of ... transmis- sion scanner (Epson, Nagano, Japan) and analyzed with imagemaster 2d platinum software, version 5.0 (GE Healthcare). Spots were detected automatically by the soft- ware...

Ngày tải lên: 15/02/2014, 01:20

... mech- anistic analysis for Dke1 [34], a Criegee rearrangement mechanism of bond cleavage by Bxe_A2876 seems unlikely. The three-histidine center of Fe 2+ in Bxe_A2876 Results of analysis of the X-ray absorption ... BW (2002) Functional analysis of the copper-dependent quercetin 2,3-dioxygenase. 2. X-ray absorption studies of native enzyme and anaerobic complexes with the...

Ngày tải lên: 18/02/2014, 06:20

... plasticity of the active site gorge of D70G compared with that of the wild-type enzyme for acylation with neutral ester. Effects of benzalkonium on ATMA hydrolysis Hydrolysis of ATMA by wild-type ... N,N,N-trimethylanilinium] and homologous esters (o-nitrophenyl acetate and acetylthiocholine). Tyramine was an acti- vator of hydrolysis for neutral substrates and an inhibito...

Ngày tải lên: 18/02/2014, 17:20

... nucleoside analogues: 1-(b-d-arabinofuranosyl)-cytosine (AraC), 1-(b-d-arabinofuranosyl)-thymine (AraT) and (E)-brom- vinyl-2¢-deoxyuridine (BVDU). Mutants of catalytic residues: E52 and R105 It ... 9-(b-d-arabinofuranosyl)-adenine (AraA), 9-(2-hydroxyethoxymethyl)-guanine (ACV) and BVDU]. The catalytic mutants E52D, E52Q, R105H and R105K did not show any detectable activity with either n...

Ngày tải lên: 19/02/2014, 02:20

... formation of PSII centers with doubly reduced Q B . This may occur simultaneously with the formation of a transmembrane voltage, as valinomycin was shown to inhibit the J–I phase of thylakoid membranes [16]. ... from thylakoid membranes. Such analysis is needed for a a more thor- ough use of FI in the study of PSII electron transport and to obtain a more complete analysis...

Ngày tải lên: 19/02/2014, 05:20

... Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin Nabil A. M. Sultan, Bhaskar G. Maiya* and Musti J. Swamy School of Chemistry, University of Hyderabad, ... significant changes in the secondary structure and tertiary structure of the protein clearly indicates that the lectin does not undergo any detectable conformational changes on binding...

Ngày tải lên: 19/02/2014, 16:20