Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase th

Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Puriﬁcation and properties docx

... His 332 -Gln 313 pair in enzyme catalysis. FEBS Lett. 390, 104–108. 17. Asano, Y. , Sekigawa, T., Inukai, H. & Nakazawa, A. (1988) Puriﬁcation and properties of formate dehydrogenase from Mor- axella sp. strain ... separated in a 10% polyacrylamide gel and stained with Coomassie brilliant blue R250. Lane A, molecular mass standards: phosphorylase B (97 kDa), albumin (66...

Ngày tải lên : 20/02/2014, 23:20

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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Puriﬁcation and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc

... A novel meta -cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Puriﬁcation and characterization of 4-amino-3-hydroxybenzoate 2,3 -dioxygenase from Bordetella sp. strain ... properties. Keywords: 4-amino-3-hydroxybenzoate- degrading bacter- ium; 2-aminophenol derivatives; meta-cleavage dioxygenase; 4-amino-3-hydroxybenzoate 2...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt

... Biochemistry, Laboratory of Cardiovascular Research, 2 Division of Cell Biology, National Institute of Biostructures and Biosystems, and 3 Department of Drug Sciences, University of Sassari, Italy; 4 Callerio Foundation, ... tumorigenic and metastatic potentials to NIH3T3 cells [11], suggest that pharmacological mani- pulation of this signal transduction pathway may repres...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: A novel, inducible, citral lyase puriﬁed from spores of Penicillium digitatum docx

... the actions of a hydratase and an aldolase are needed. Citral lyase of P. digitatum combines hydratase and aldolase activity in a single enzyme. No other enzyme has been reported to catalyse ... hydratase and aldolase activity (A) , from P. digitatum and other (B) and (C) hydratase/ aldolase enzymes described in literature. (B1) enoyl-CoA hydratase/ aldolase [26]; (B2) tra...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Characterization of a novel silkworm (Bombyx mori ) phenol UDP-glucosyltransferase potx

... Technology and Medicine, London SW7 2AZ, UK; 2 Laboratory of Molecular Entomology and Baculovirology, Riken, Wako, Japan Sugar conjugation is a major pathway for the inactivation and excretion of both ... project. A wing disc cDNA library derived from ﬁfth instar B. mori C108 larvae was kindly provided by Dr Kawasaki (University of Utsunomiya, Japan). A total of 1000 clo...

Ngày tải lên : 22/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Mammalian mitotic centromere-associated kinesin (MCAK) A new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents pptx

... Takemura M, Sahara H, Takahashi N, Gasa S, Sugawara F, Matsuk- age A, Yoshida S & Sakaguchi K (1998) Studies on inhibitors of mammalian DNA polymerase alpha and beta: sulfolipids from a pteridophyte, ... membrane was stained with anti-His 6 Ig and alkaline phosphatase. A single band was present that corresponded to the molecular mass of MCAK184 (49 kDa). S. Aoki et al. A...

Ngày tải lên : 19/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... normal enzyme. Eur J Biochem 246, 548–556. 15 Ikeda Y, Okamura-Ikeda K & Tanaka K (1985) Puriﬁ- cation and characterization of short-chain, medium- chain, and long-chain acyl-CoA dehydrogenases ... impact greatly on the catalytic activity, as the k cat and the ETF interaction are relatively unaffected. This mutation seems to affect mainly the initial folding and stability of...

Ngày tải lên : 20/02/2014, 02:21

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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is speciﬁcally induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... b-1,4-Endogalactanases cleave within the galactan moiety of type I arabinogalactan, releasing D -galacto-oligosaccharides. Bacterial b-1,4-endogalactanases release mainly galactotriose and galactotetra- ose ... detected against any of the transgalactooligosaccharides listed in Materials and methods. Hydrolysis of arabinogalactans The sugar composition of the arabinogalactans fr...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

... Biochemistry 39, 7131– 7137. 31. Hiraoka, B .Y. , Yamakura, F., Sugio, S. & Nakayama, K. (2000) A change of the metal-speciﬁc activity of a cambialistic superoxide dismutase from Porphyromonas gingivalis ... mutagenesis reactions together with oligonucleo- tides ECF-Q69G d(5¢-AACAACGCAGCT GGGCTCTG GAACCAT), ECF -A1 41Q d(5¢-TCAACCTCTAAC CAG GCTACTCCGCTG) ECM-G77Q d(5¢-AACAACGCTG...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization ﬁeld pptx

... various characterization methods: fractionation by size-exclusion and anion-exchange HPLC, amino acid analysis, glycosaminoglycan and calcium quantiﬁcation, SDS/PAGE and FTIR spectroscopy. ... WSM. Glycosaminoglycan analysis Glycosaminoglycans are highly negatively charged because of the presence of sulfate ester and/ or carboxyl groups. Therefore, they interact with cations a...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Puriﬁcation and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp strain 10d doc

Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Puriﬁcation and properties docx

Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Puriﬁcation and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc