Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... staining of cystatin F and ER. (A) Cystatin F was stained with a human cystatin F specific polyclonal antiserum and a FITC labelled secondary antibody (green sig- nal). (B) Following the cystatin F staining, ... cystatin F produced by U937 cells is retained intracellularly or is taken up by the cells immedi- ately following secretion. Accordingly, the relative...

Ngày tải lên: 21/02/2014, 01:21

... malaria and in the brain of a murine model of the syndrome [18,19]. Interestingly, picolinate is reportedly able to prevent the neurotoxic effects of quinolinate in the rat central nervous system, ... suc- cessfully restored the bacterial protein [21,33]. Interestingly, the same difficulty in stripping off the metallocofactor from the protein has been described for murine adenosi...

Ngày tải lên: 19/02/2014, 02:20

... substitution of glycine residues by alanine or proline and the exchange of proline by glycine. Glycine residues are frequently found at the C-terminal ends of a helices providing the conforma- tional ... by site-directed mutagenesis of FHT [7,9] and by documentation of ligand binding in crystalline DAOCS and IPNS complexes [23–25]. The coordination of Fe II is commonly med...

Ngày tải lên: 21/02/2014, 03:20

... pGL3-Enhancer (Promega) and then utilized for LipofectAMINE Plus- mediated transien t transfection of CV1 ce lls followed 24 h later by analysis of luciferase activity utilizing the Lucife rase Assay System o f Promega. ... by heat- ing a 4-l M mixture of primers to 95 °C for 3 min followed by cooling to 22 °C prior to cloning into the Xho1/Sal1 sites of vector pEGFP-N3. Integrity...

Ngày tải lên: 19/02/2014, 16:20

... kDa and  60 kDa isoforms of Ras-GRF1 (Fig. 2A, left panel) and of recombinant HA-Ras-GRF1 (Fig. 2B, upper panel). Phosphorylation of ERK1 ⁄ 2 in the same sam- ples was fully induced after 3 min ... increase phosphorylation of endogenous Ras-GRF1 in rat brain [9]. Serotonin-induced phosphorylation of Ras-GRF1 is dependent on cAMP and PKA Serotonin increases adenylyl cyclas...

Ngày tải lên: 19/02/2014, 18:20

... that obtained in the presence of Mn 2+ (Table 1). An independent estimation of the binding affinity of PEP for E. coli PEP carboxykinase was obtained from the quenching of the emission of Trp residues ... only in the presence of Mn 2+ . This high binding affinity of PEP in the presence of Mn 2+ appears common to PEP- carboxykinases. A K d of 0.6 l M has been determined f...

Ngày tải lên: 21/02/2014, 01:21

... reactivity of, Cys421 towards a second molecule. Alternatively, biphasic inactivation by 1a (with two inacti- vation rates differing by a factor of 10) may originate from the different accessibility of ... were recently introduced for the preparation of C-6 aldehyde derivatives of Glc [15]. The key step is the use of Collins reagent for the selective oxidation of the primary trim...

Ngày tải lên: 21/02/2014, 01:21

... was from Fluka Chemie AG, Switzerland. Phenylmethanesulfonyl fluoride and okadaic acid were from Sigma. Trypsin of modified sequencing grade was obtained from Promega, and LysC of Achromobacter lytii ... liver cytosol For small-scale preparation of cytosol, 10 g of fresh porcine liver obtained from the local abattoir was homogenized for 1 min at 600 r.p.m. in three volumes of ho...

Ngày tải lên: 21/02/2014, 01:21

... further evidence of the formation of the RuvAC-complex. Fig. 6. SPR sensorgram showing the DNA binding specificity of RuvA. (A) Binding of wild-type RuvA (2 l M ) and (B) binding of the mutant RuvA ... different flow cells on a streptavidin sensor chip. The binding of 2 l M E. coli RuvA with the different complexes is shown in Fig. 6A. The difference in stability of the du...

Ngày tải lên: 21/02/2014, 01:21

... cata- lyses the hydrolysis of agmatine to putrescine and urea [1]. Agmatine, which results from decarboxylation of arginine by arginine decarboxylase [2], is a metabolic intermediate in the biosynthesis ... according to the instructions of the manufacturer. A single protein band was detected by SDS/PAGE of purified enzymes. Enzyme activities were determined by measuring the formati...

Ngày tải lên: 21/02/2014, 01:21