Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

... al. (Eur. J. Biochem. 269) Ó FEBS 2002 Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue The ´ re ` se ... Biochemistry 39, 7131– 7137. 31. Hiraoka, B .Y. , Yamakura, F., Sugio, S. & Nakayama, K. (2000) A change of the metal-specific activity o...

Ngày tải lên: 21/02/2014, 01:21

... chondroitin/dermatan sulfate PGs at the d and e bands in the gap zone of the fibrils formed by the quarter staggered array of type I collagen molecules, and the presence of keratan sulfate PGs at the a and ... was then added to 50 m M final concentra- tion and the samples were incubated for 1 h, extensively dialyzed against NaCl/P i andstoredat)20 °C. Preparation...

Ngày tải lên: 21/02/2014, 15:20

... with the forward primer: 3¢-GTCACAGAGCCACAGTTCCAG CCAGGA-5¢. Codon 305 of C-YES was mutated from AAA (Lys) to AGA (Arg) with the forward primer: 3¢-GG AACCACGAAAGTAG CAATC AGA ACACTA AAACC A GGTACAATGATGC-5 ... kinase of the Src family that is expressed in a wide range of tissues [24]. The N-terminus of C-YES is dually myristoylated and palmitoylated [25], and th...

Ngày tải lên: 18/02/2014, 18:20

... Medical School, Department of Biochemistry and Molecular Biology, Bunkyo-ku, Tokyo, Japan; 3 Osaka Medical School, Department of Physics, Takatsuki, Osaka, Japan AsystematicsearchforEscherichia coli ... (A and C, 1 lg; B and D, 5 lg), BSA (A and C, 1 lg; B and D, 5 lg), RNA polymerase (A and C, 1 lg; B and D, 5 lg) and RpoD (RNA polymerase r subunit) (A and C, 1 lg...

Ngày tải lên: 22/02/2014, 04:20

... developed after the delay. The delayed signal was inhibited by concomitant addition of SOD and the metal chelator DTPA. These data show that initial PBN adducts are dependent on the superoxide anion radical ... to the presence of the thiyl radical in the aerobic system. First, the complexes are inactivated by 2-oxo acid and CoA in the presence of O 2 (Fig. 2A)...

Ngày tải lên: 21/02/2014, 01:21

... (GeneBank accession num- ber AI975843). A 549-bp fragment was amplified by PCR using oligonucleotides GSTX1: 5¢- GTTGTCGACAAA CATCTCAACTAG-3 and GSTX3: 5¢-GTAAGTGTGG GAATAAGATCAAATC from adult S. mansoni ... here the characterization of novel dehydroascorbate reductase and thiol transferase activities that reside in the human parasite Schistosoma mansoni GSTx. Protein sequence...

Ngày tải lên: 21/02/2014, 01:21

... our laccase oxidations of substrates 1–5, and the effect of the cosolvent and of the mediators on the amount of substrate metabolized by the enzyme. (a) Laccase and laccase/HPI. In general, the ... determination of laccase activity The activity of laccase was determined by following the rate of oxidation of 2,2¢-azinobis-(3-ethylbenzothiazoline-6-...

Ngày tải lên: 21/02/2014, 01:21

... 5¢-ATGTTAAA GAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and 5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAG CCG-3¢, cloned into the expression vector pBAD-TOPO, transformed into the E.coliTop10 strain and grown on LB agar ... triose-phosphate isomerase [24], a- amylase [27,28], and that of malate dehydrogenase [31] have been published. The psychrophilic enzymes characterized so far generally have hig...

Ngày tải lên: 21/02/2014, 01:21

... GAPDH mRNA was utilized as a constant mRNA for control. Statistical analysis The statistical analysis of the data was performed using the unpaired Student’s t-test, assuming a P < 0.05 as the ... that pharmacological mani- pulation of this signal transduction pathway may represent a promising strategy for handling of human diseases including cancer and metastasis. New...

Ngày tải lên: 21/02/2014, 01:21

... to the partially negat- ively charged oxygen of Gln, leading to the observed effect. Analysis of activation parameters This analysis was based on the transition state theory of Eyring [37]. The ... three-dimensional structure of plastocyanin is highly conserved among plants and cyanobacteria, the surface charge pattern varies greatly [1]. Comparing cyanobacterial data wi...

Ngày tải lên: 21/02/2014, 01:21