... electrophoretically to a poly(vinylidenedifluoride) membrane. The membrane was treated with a rabbit anti-iNOS polyclonal antibody or rabbit anti-PKCpolyclonal antibody at 1 : 1000 dilution and anti-(rabbitIgG) ... 378S–381S.7. Badamchian, M., Spangelo, B.L., Bao, Y. , Hagiwara, Y. ,Hagiwara, H., Ueyama, H. & Goldstein, A. L. (1994) Isolation of a vitamin E analog from a green barley leaf extract that stimulatesrelease ... horseradish peroxidase conjugated antibody at1 : 5000 dilution as a primary antibody and a secondaryantibody, respectively. The blots were detected with an enhanced chemiluminescence kit (Amersham...