Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions Ronald ... consists of 99% D -galactose and 0.3% L -arabinose and is predominantly linear. Potato arabinogalactan consists of 86% D -galactose...

Ngày tải lên: 21/02/2014, 01:21

... by centrifugation, and the supernatant was analyzed to determine its alanine content by performing an alanine dehydrogenase assay [50]. The alanine content of assay mixtures was determined based ... that is highly similar to that of IscS (Fig. 1A) , with a two-domain organization and the presence of both a- helices and b-strands. On the basis of these data and...

Ngày tải lên: 18/02/2014, 04:20

... that dephosphorylation and/ or catalytic turnover of EII Glc , rather than binding of Glc, enhanced the reactivity of Cys421. As Cys421 is the only invariant cysteine in homologous transporters and also the only ... binding and/ or translocation of substrates via the periplasmic site. EII Glc is rapidly inactivated by the 6-O-bromoacetyl esters of methyl a- D -...

Ngày tải lên: 21/02/2014, 01:21

... Council of Canada, Ottawa, Canada Campylobacter jejuni infections are one of the leading causes of human gastroenteritis and are suspected of being a pre- cursor to Guillain–Barre ´ and Miller–Fisher ... missing in the latter [6]. As already mentioned, the missing galactose can be attributed to the phase variability of wlaN. The lack of terminal GalNAc in this...

Ngày tải lên: 21/02/2014, 01:21

... the concentration of analyte, R max is the maximum analyte binding capacity in RU and R is the SPR signal in RU at time t, k a the association rate constant and k d the dissociation rate constant. Using ... constant. Nonlinear regression analysis was used to determine the equilibrium dissociation constant from the sensorgrams and allows the calculation of...

Ngày tải lên: 21/02/2014, 01:21

... histone deacetylase activity and to perhaps function through a mechanism that depends on interactions with components of the basal transcriptional machinery such as TATA binding protein and transcription ... shows that yeast har- bouring an expression vector encoding a Gal4 activation domain–Eos-mut fusion and a Gal4 DNA-binding domain–CtBP fusion grow in the absence...

Ngày tải lên: 21/02/2014, 01:21

... membranes, and of causing the lysis of A. laidlawii B and human erythrocytes, are at least qualitatively correlated. As discussed earlier, GS10 and GS14 both exist in aqueous solution as antiparallel ... Data acquisition and analysis was carried out using MICROCAL DA -2 (MicroCal LLC, Northampton, MA, USA) and ORIGIN software (OriginLab Corporation, Northampton, MA, USA). S...

Ngày tải lên: 21/02/2014, 01:21

... methods. Enzyme activity was a nalysed by the standard assay and the i mmunoreactivity was detected by Western blot analysis and quantitated as described i n Materials and methods. Open and filled ... subtilases h ave about 20 amino acids in this region and the large in sertion is a special feature of TPP II and pyrolysin [9,21]. There are, in total, 12 amino -a...

Ngày tải lên: 21/02/2014, 15:20

... results indicate that the increase in the affinity of NK to chromatin is an ATP-dependent reaction, and is caused by a kinase(s) in the c ytosol. Then, authentic p rotein kinase A (PKA) and calmodulin-dependent ... N-terminal and arginine-serine repeat-containing regions to chromatin was suppressed by mild trypsinization of the chromatin and by pretreatment...

Ngày tải lên: 22/02/2014, 04:20

... electrophoretically to a poly(vinylidene difluoride) membrane. The membrane was treated with a rabbit anti-iNOS polyclonal antibody or rabbit anti-PKC polyclonal antibody at 1 : 1000 dilution and anti-(rabbit IgG) ... 378S– 381S. 7. Badamchian, M., Spangelo, B.L., Bao, Y. , Hagiwara, Y. , Hagiwara, H., Ueyama, H. & Goldstein, A. L. (1994) Isolation of a vitamin E analog fro...

Ngày tải lên: 22/02/2014, 04:20