Tài liệu Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase pptx

... 4325 Kinetics and thermodynamics of nick sealing by T4 DNA ligase Alexey V. Cherepanov* and Simon de Vries Kluyver Department of Biotechnology, Delft University of Technology, the Netherlands T4 DNA ligase ... (T4 DNA ligase was 0.1 l M ). Ó FEBS 2003 Kinetics of nick sealing by T4 DNA ligase (Eur. J. Biochem. 270) 4319 In the case of joi...

Ngày tải lên: 21/02/2014, 00:20

... Composition of nucleosomes. The assembly of the histone octamer on DNA is represented by this model which depicts the incorporation of two H3 ⁄ H4 dimers with an inner core of  60 bp of DNA, followed by ... two turns of the DNA helix, by contacting the inner core DNA within one half of the nucleosome plus the DNA at the exit point of the opposite half. P. N. Co...

Ngày tải lên: 14/02/2014, 18:20

... expression of estrogen receptor-a (ERa) is autoregulated by miR-18a, -19b and -20b, which in turn are upregulated by the activation of ERa. This mechanism of regulation provides a wide range of coordinated ... Scientific Fund of the Ministry of Education, Science and Culture of Japan (G.T.); the Program for Promotion of Fundamental Studies in Health Sciences of the Na...

Ngày tải lên: 14/02/2014, 19:20

... number of hours, and the relative mRNA levels of IL-1b and TNF-a were determined by real-time PCR. The data are the mean ± SEM of at least three independent experiments. *P < 0.05 and **P ... exposed to BAY 11-7082 or MG132, followed by hypoxia ⁄ SD for 6 h and detection of relative mRNA levels of IL-1b and TNF-a by real-time PCR. *P < 0.05 and **P < 0.01...

Ngày tải lên: 18/02/2014, 04:20

... behave differently from the rest of the promoters. Of these, amino acid residues 243 and 275 are involved in DNA binding and 244 and 246 are in very close proximity to DNA binding. This indi- cates ... advantage of p53 mutation analysis, and a unique feature of this database, is the availabil- ity of the residual activity of the majority of p53 mis- sense mutants. Th...

Ngày tải lên: 18/02/2014, 11:20

... presented by a mixture of clo- sely related isoforms. In the present study, four pulchel- lin isoforms were isolated, and their amino acids sequences deduced by cDNA cloning and verified by MS. Half ... M r values of the standards in thousands. Lanes 2–9, 5 lg of peak P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec- tively, in the presence (lanes 2–5) or...

Ngày tải lên: 18/02/2014, 16:20

... 3673 Expression of Noxo1b and Noxo1c in various human tissues The expression patterns of the Noxo1b and Noxo1c mes- sengers were determined by PCR using Human Multiple Tissue cDNA panels and Human ... Functional Analyses from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and CREST and BIRD projects of JST (Japan Science and Technology A...

Ngày tải lên: 19/02/2014, 06:20

... assay. Stereospecificity and substrate specificity The stereospecificity of T. reesei TYR2 was studied by following the activities on 15 mml-dopa, dl-dopa and d-dopa and 2.5 mml-dopa, dl-dopa and d-tyrosine, and the ... Buchert 1 and Kristiina Kruus 1 1 VTT Technical Research Centre of Finland, Espoo, Finland 2 Protein Chemistry Research Group and Core Facility, Institute of...

Ngày tải lên: 19/02/2014, 06:20

... step. Isolation of PsCIPK and PsCBL cDNA clones and their sequence analysis Basically, both CIPK and CBL cDNAs were first partially cloned by PCR and then followed by isolation of full-length clones by library ... the cytosol. Results Isolation and sequence analysis of PsCIPK and CBL cDNAs and genomic clones For cDNA cloning, first partial fragments of 550 bp for PsC...

Ngày tải lên: 19/02/2014, 07:20

... Branch of the National Research Institute of Aquaculture (Tochigi, Japan) and reared on a commercial diet at 15 °C. Cloning of HSF cDNA A random primed kZAPII cDNA library was constructed by using ... instructions. The 5¢ -and3 ¢-termini of rainbow trout HSF cDNAs were isolated by RACE. A directional cDNA library was constructed from RTG-2 cells by using a SuperScript Plasmid...

Ngày tải lên: 19/02/2014, 12:20