Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf

... oligomerization of human D 2 dopamine receptors expressed in Spodoptera frugiperda 9( Sf9 ) and in HEK 293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy ... D 2 receptor oligomerization. Intact Spodoptera frugiperda 9( Sf 9) cells expressing FLAG-tagged dopamine D 2L receptors (A) or FL...

Ngày tải lên: 21/02/2014, 00:20

... Ala, mGFP–calpain 7 (WT), mGFP–calpain 7 C 290 S (C 290 S) and mGFP–calpain 7 C 290 A (C 290 A) were transiently expressed in HEK 293 T cells, and total cell lysates were then ana- lyzed by WB with mAb against GFP and pAb ... mGFP–calpain 7–Strep and mGFP–calpain 7 C 290 S –Strep from HEK 293 T cells. Cleared lysate of untransfected HEK 293 T cells (mock) and those of c...

Ngày tải lên: 18/02/2014, 04:20

... group of thymidine, forming dTMP. Human TK1 is cytosolic and its activity is absent in resting cells, appears in late G 1 , increases in S phase coinciding with the increase in DNA synthesis, and disappears ... Tyrsted G ( 199 5) Human thymidine kinase 1. Regulation in normal and malignant cells. Adv Enzyme Regul 35 , 69 89. 25 Munch-Petersen B, Cloos L, Tyrsted G...

Ngày tải lên: 18/02/2014, 12:20

... hormone-binding domain of the murine estrogen receptor (amino acids 281– 599 ), the cDNA for the 9E10-epitope of c-Myc (amino acids 410–4 1 9) and the cDNA for a remainder of c-Myb (amino acids 188– 199 ) into ... reaction and religation. pmyc ()2 24 ⁄ )9 2 )mintkluc was made from pmyc ()2 62 ⁄ )9 2 )mintkluc by BamHI (partial) ⁄ BstYI diges- tion and religation. pmy...

Ngày tải lên: 19/02/2014, 05:20

... Moroni C ( 199 9) Characterisation and mitochondrial localisa- tion of AUH, an AU-specific RNA-binding enoyl-CoA hydratase. Gene 228, 85 91 . 17 Nakagawa J & Moroni C ( 199 7) A 20-amino-acid autonomous ... RNA-binding domain contained in an enoyl-CoA hydratase. Eur J Biochem 244, 890 – 899 . 18 Kurimoto K, Fukai S, Nureki O, Muto Y & Yokoyama S (200 1) Crystal structure...

Ngày tải lên: 19/02/2014, 07:20

... 4-aminoquinolines. J. Am. Chem. Soc. 69, 1 091 –1 099 . 30. Zamyatnin, A.A. ( 197 2) Protein volume in solution. Prog. Biophys. Mol. Biol. 24, 1 09 123. 31. Karplus, P.A. ( 199 7) Hydrophobicity regained. ... regained. Protein Sci. 6, 1302–1307. 32. Main, A.R. ( 197 9) Mode of action of anticholinesterases. Phar- macol. Ther. 6, 5 79 628. 33. Fersht, A. ( 199 9) Structure...

Ngày tải lên: 20/02/2014, 23:20

... deamination of primary and secondary amines (sarcosine, N-ethylgly- cine, and glycine) and d-amino acids (d-alanine and d- proline), yielding the corresponding a-keto acid, ammonia or primary amine ... improved bind- ing to longer ligands (up to five atoms in the main chain) and retained binding to shorter ligands [55]. Of the 21 changes in the evolution of R7 from native G...

Ngày tải lên: 14/02/2014, 14:20

... PXXP in interacting pro- teins. Seven such motifs are present in the proline-rich domain of the longest isoform of tau in the human central nervous system (CNS). Two distinct PXXP motifs in tau, ... and cultured in Neurobasal medium (Invitrogen) containing 2% (v ⁄ v) B-27 serum-free supplement, 0.5 m ML-glutamine, 100 UÆmL )1 penicillin, and 100 lgÆmL )1 streptomyc...

Ngày tải lên: 14/02/2014, 14:20

... (2.6–2. 5) No. of reflections 334 560 ( 49 27 0) No. of unique reflections 87 766 (12 78 7) Completeness ( %) 99 .9 (99 . 9) I ⁄ r (I) 5.8 (2 . 9) Multiplicity 3.8 (3 . 9) R merge 25.8 (44. 1) R pim 15.3 (26. 0) Analysis Statistics ... 4.5 E° = 0. 69 V pH 6 E° = 0.65 V rMaL (E° = 0.48 V) DE° (V) ) 0.05 0.08 ) 0. 09 ) 0.03 ) 0.21 ) 0.17 K m...

Ngày tải lên: 14/02/2014, 18:20

... backbone: Asn146 ()4 .8 sc ); Asn188 ()2 .4 sc ); Asn235 ()2 .0 sc ); Trp142 ()2 .2 sc ); Trp184 ()3 .9 sc ). In addition, Ser1 49 ()3 .3 sc ) is notably constrained by the CLIC4 NLS in a single conforma- tion ... B (201 0) Probing the specificity of binding to the major nuclear localization sequence-binding site of importin-alpha using oriented...

Ngày tải lên: 14/02/2014, 19:20