Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalli

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... chains. Both have been implicated in the binding of ACP [2], probably by donating hydrogen bonds to one of the carbonyl oxygens in malonyl-ACP, thereby stabilizing charge relocalization during ... ‘bulky’ mutants all point to the orientation of H333 and its hydrogen bond donor properties as being the cornerstone of obtaining and ⁄ or retaining residual decarboxylase activity. The emer...

Ngày tải lên : 19/02/2014, 08:20

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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... high-molecular- weight; KIND, kinase noncatalytic C-lobe domain; KIND1, ﬁrst kinase noncatalytic C-lobe domain; KIND2, second kinase noncatalytic C-lobe domain; MAP2, microtubule-associated protein 2; GEF, guanine ... EGFP- KIND2-1 to -KIND2-5 (Fig. 4A), by a pull-down assay with GST-CD2 (MAP2 v-KIND binding core). Input (cerebellum) GST v-KIND KIND1 v-KIND KIND2 Spir-2 K...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays. In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ... a (encoded by CKA1) and a¢ (encoded by CKA2), and two regulatory b subunits, b and b in Saccharomyces cerevisiae (CKB1 and CKB2). The enzyme oc...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx

... FEBS 2003 Insulin upregulates metastasis via PKB and SLe x (Eur. J. Biochem. 270) 3805 Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 ... metastasis-related phenotypes and molecules via protein kinase B, but not PI-3K, in H7721 human hepato-carcinoma cell line. Mol. C...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Casein kinase 2 speciﬁcally binds to and phosphorylates the carboxy termini of ENaC subunits ppt

... determined by assessing effects of various point mutations on 32 P incorporation into GST -b and -c. Accordingly, several serines and threonines on the C termini of b and c weremutatedtoalanineandexamined for ... termini of a, b, andcENaC were phosphorylated by fraction 76. Autoradiogram and Coomassie blue staining are shown. Fig. 2. Binding of kinases to GST -b and -c. GST, GS...

Ngày tải lên : 21/02/2014, 03:20

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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... is divided into three subfamilies: antiapoptotic proteins (Bcl-2, Bcl-xL, Bcl-w, Mcl-1 and A1), pro-apoptotic proteins (Bax, Bak and Bok) and BH3-only proteins (Bad, Bid, Bik, Blk, Hrk, BNIP3 and BimL). ... to mitochondria and the binding of Bcl-xL [86], and increases the release of cytochrome c from mitochondria into the cytosol through inactivation with Bcl-xL and Bcl-2 [69]...

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium pptx

... and disassembly of cadherin–catenin complexes The cytoplasmic domain of cadherin interacts with F-actin via b- catenin and a-catenin; b- catenin binds to cadherin and a-catenin, which in turn interacts ... b- catenin [25,26], which leads to disassembly of the cadherin–catenin complex. b- Cate- nin is directly tyrosine-phosphorylated by the nonre- ceptor protein tyrosine kinase <...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc

... of ubiquitin-tagged proteins [41]. Ubiquitination of proteins is catalysed by three enzymes: ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2) and ubiquitin protein ligase ... total protein in the cultures increased with time (Student’s t-test, P < 0.05). In both cultures, cell numbers increased over time (Fig. 1B) . However, the cell num- ber increased expone...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Dimers of light-harvesting complex 2 from Rhodobacter sphaeroides characterized in reconstituted 2D crystals with atomic force microscopy docx

... acidophila, but was caused by speciﬁc interactions induced by packing. In contrast, tilted LH2’s and RC–LH1’s have been observed in native membranes of Rhodob. sphaeroides [17]. Moreover, in the aforementioned model ... functionalized invaginations of the intracytoplasmic membrane containing the photosynthetic machinery. The most abundant protein complexes in the intracytoplasmic...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... min and 0.5% saponine in NaCl ⁄ P i for 10 min. Permeabilized cells were blocked for 20 min with 10% horse serum in NaCl ⁄ P i , and then incubated for 60 min with a rabbit polyclonal anti-dinitrophenyl ... CT–ARF interaction has been extensively characterized in vitro, little is known about their in vivo interaction, and the subcellular binding site(s) between CT and ARF-6 re...

Ngày tải lên : 19/02/2014, 02:20

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